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Emerg Microbes Infect
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Quantitative proteomic analysis of MDCK cell adhesion

Molecular Omics, 2021
Establishing a stable MDCK suspension cell line by genetic engineering has significant potential to aid industrialization of vaccine production. In this study, quantitative proteomics was used to explore adhesion proteins in MDCK cells.
Xuanqing Ye, Jiamin Wang, Zilin Qiao, Di Yang, Jiao Wang, Ayimuguli Abudureyimu, Kun Yang, Yuping Feng, Zhongren Ma, Zhenbin Liu   +9 more
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Hydraulic Properties of MDCK Cell Epithelium

Journal of Membrane Biology, 1996
The water permeability of the apical and basolateral cell membranes and the compliance of the lateral intercellular spaces (LIS) of MDCK monolayers were measured on confluent cultures grown on permeable supports. Cell membrane water permeabilities were determined, using quantitative differential interference light microscopy, from the rate of cell ...
M M, Timbs, K R, Spring
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Apical membrane permeability of MDCK cells

American Journal of Physiology-Cell Physiology, 1996
The osmotic water permeability (Pf) and permeability to nonelectrolytes were determined for the apical membrane of clonal strain Madin-Darby canine kidney (MDCK) C12 cells cultured as cysts with the apical membrane facing the surrounding medium. Pf and solute permeabilities were calculated from the rate of volume change of cysts by digitizing images ...
R L, Rivers, J A, McAteer, J L, Clendenon, B A, Connors, A P, Evan, J C, Williams   +5 more
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Cytomatrix synthesis in MDCK epithelial cells

Journal of Cellular Physiology, 1990
AbstractDetailed information regarding the synthesis rates of individual protein components is important in understanding the assembly and dynamics of the cytoskeletal matrix of eukaryotic cells. As an approach to this topic, the dual isotope technique of Clark and Zak J. Biol.
J J, Mitchell, R B, Low, J L, Woodcock-Mitchell   +2 more
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Comparison of bidirectional lamivudine and zidovudine transport using MDCK, MDCK–MDR1, and Caco-2 cell monolayers

Journal of Pharmaceutical Sciences, 2009
Bidirectional transport studies were conducted using Caco-2, MDCK, and MDCK-MDR1 to determine P-gp influences in lamivudine and zidovudine permeability and evaluate if zidovudine permeability changes with the increase of zidovudine concentration and/or by association of lamivudine.
Jacqueline de Souza, Leslie Z Benet, Fcp
exaly   +3 more sources

Integrin expression and localization in normal MDCK cells and transformed MDCK cells lacking apical polarity

Journal of Cell Science, 1994
ABSTRACT Epithelial cells polarize in response to contacts with the extracellular matrix and with neighboring cells. Interac-tions of cells with the extracellular matrix are mediated mainly by the integrin family of receptors. To begin to understand the role of integrins in polarization, we have investigated the expression and ...
Schoenenberger, C. A., Zuk, A., Zinkl, G. M., Kendall, D., Matlin, K. S.   +4 more
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Electrical properties of cultured epithelioid cells (MDCK)

The Journal of Membrane Biology, 1983
This is a study of the intracellular electrical potential, membrane resistance, and capacity of MDCK cells (epithelioid of renal origin) cultured in monolayers on a collagen couch. These monolayers have a transepithelial resistance of 256 +/- 12 (22) ohm cm2 (mean +/- standard error, and number of observations), and the cells have 61.6 +/- 6.3 (92) M ...
E, Stefani, M, Cereijido
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Methods for Establishing Rab Knockout MDCK Cells

2021
The Rab family small GTPases are key regulators of intracellular membrane traffic that are conserved in all eukaryotic cells. Rabs are thought to regulate various steps of membrane traffic, including the budding, transport, tethering, docking, and fusion of vesicles or organelles.
Riko, Kinoshita, Yuta, Homma, Mitsunori, Fukuda   +2 more
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The Apical Membrane Glycocalyx of MDCK Cells

The Journal of Membrane Biology, 2000
The microenvironment near the apical membrane of MDCK cells was studied by quantitation of the fluorescence of wheat germ agglutinin attached to fluorescein (WGA). WGA was shown to bind to sialic acid residues attached to galactose at the alpha-2,3 position in the glycocalyx on the apical membrane.
O N, Kovbasnjuk, K R, Spring
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