Sialylated and sulfated N-Glycans in MDCK and engineered MDCK cells for influenza virus studies [PDF]
Scientific Reports, 2022 The Madin-Darby canine kidney (MDCK) cell line is an in vitro model for influenza A virus (IAV) infection and propagation. MDCK-SIAT1 (SIAT1) and humanized MDCK (hCK) cell lines are engineered MDCK cells that express N-glycans with elevated levels of ...
Lauren Byrd-Leotis +6 more
doaj +4 more sources
Claudin-2 knockout by TALEN-mediated gene targeting in MDCK cells: claudin-2 independently determines the leaky property of tight junctions in MDCK cells. [PDF]
PLoS ONE, 2015 Tight junctions (TJs) regulate the movements of substances through the paracellular pathway, and claudins are major determinants of TJ permeability. Claudin-2 forms high conductive cation pores in TJs.
Shinsaku Tokuda, Mikio Furuse
doaj +2 more sources
Metabolomics profiling reveals differences in proliferation between tumorigenic and non-tumorigenic Madin-Darby canine kidney (MDCK) cells [PDF]
PeerJ, 2023 Background Madin-Darby canine kidney (MDCK) cells are a cellular matrix in the production of influenza vaccines. The proliferation rate of MDCK cells is one of the critical factors that determine the vaccine production cycle.
Na Sun +11 more
doaj +3 more sources
TGM2 inhibits the proliferation, migration and tumorigenesis of MDCK cells. [PDF]
PLoS ONE, 2023 Madin-Darby canine kidney (MDCK) cells are one of the main cell lines used for influenza vaccine production due to their high virus yield and low mutation resistance.
Zhenyu Qiu +12 more
doaj +2 more sources
High yield production of influenza virus in Madin Darby canine kidney (MDCK) cells with stable knockdown of IRF7. [PDF]
PLoS ONE, 2013 Influenza is a serious public health problem that causes a contagious respiratory disease. Vaccination is the most effective strategy to reduce transmission and prevent influenza.
Itsuki Hamamoto +3 more
doaj +2 more sources
TRAF3 deficiency in MDCK cells improved sensitivity to the influenza A virus [PDF]
Heliyon, 2023 Tumor necrosis factor receptor-associated factor 3 (TRAF3), an adaptor protein, has significant and varying effects on immunity depending on cell types. The role of TRAF3 in Madin-Darby Canine Kidney Epithelial (MDCK) cell resistance to influenza A virus
Yang Le +14 more
doaj +2 more sources
Influenza and Other Respiratory Viruses, 2017 Two new subclades of influenza A(H3N2) viruses became prominent during the 2014‐2015 Northern Hemisphere influenza season. The HA glycoproteins of these viruses showed sequence changes previously associated with alterations in receptor‐binding properties.
Yipu Lin, John Mccauley, Rod Daniels
exaly +2 more sources
Tumorigenicity decrease in Bcl-xL deficient MDCK cells ensuring the safety for influenza vaccine production. [PDF]
PLoS ONEMadin-Darby canine kidney (MDCK) cells are the recognized cell strain for influenza vaccine production. However, the tumorigenic potential of MDCK cells raises concerns about their use in biological product manufacturing.
Jiahao Zheng +11 more
doaj +2 more sources
Reduction in Interferon-Stimulated Genes Contributes to High-Yield Production of Influenza Virus in Suspension MDCK Cells [PDF]
VaccinesCompared with the traditional vaccine produced in embryonated chicken eggs, cell-based manufacturing represented by the Madin–Darby canine kidney (MDCK) cell line has a larger production scale and reduces the risk of egg shortage in a pandemic ...
Qi Wang +9 more
doaj +2 more sources
Development of high-growth influenza H7N9 prepandemic candidate vaccine viruses in suspension MDCK cells [PDF]
Journal of Biomedical Science, 2020 Background Influenza vaccine manufacturers traditionally use egg-derived candidate vaccine viruses (CVVs) to produce high-yield influenza viruses for seasonal or pandemic vaccines; however, these egg-derived CVVs need an adaptation process for the virus ...
Tsai-Teng Tzeng +11 more
doaj +2 more sources