Results 161 to 170 of about 33,848 (196)
Some of the next articles are maybe not open access.
Influence of glucose on the toxicity of oxophenylarsine in MDCK cells
Archives of Toxicology, 1995Trivalent arsenicals like oxophenylarsine (PhAsO) inhibit cellular pyruvate dehydrogenase, thus leading to a drop of acetylCoA formation and a slow-down of the citric acid cycle. Glucose may protect cells from arsenic toxicity, because increased glycolysis may prevent fatal shortage of ATP.
B, Liebl +5 more
openaire +2 more sources
Methods for Establishing Rab Knockout MDCK Cells
2021The Rab family small GTPases are key regulators of intracellular membrane traffic that are conserved in all eukaryotic cells. Rabs are thought to regulate various steps of membrane traffic, including the budding, transport, tethering, docking, and fusion of vesicles or organelles.
Riko, Kinoshita +2 more
openaire +2 more sources
Electrical Properties of MDCK Cells
1985The electrical characterization of a transporting epithelium requires information three main aspects: (1) the overall electrical properties across the whole cell layer; (2) the transcellular permeation route; and (3) the paracellular pathway. In the present article we describe assumptions, techniques, efforts, and information collected on these aspects
L. González-Mariscal +4 more
openaire +1 more source
Polarized trafficking of thyrocyte proteins in MDCK cells
Molecular and Cellular Endocrinology, 2002Recent studies suggest striking similarities between polarized protein sorting in thyrocytes and MDCK epithelial cells, including apical trafficking of thyroglobulin (Tg), thyroid peroxidase, and aminopeptidase N; as well as basolateral targeting of heparan sulfate proteoglycans, thrombospondin 1 (TSP1), type 1 5'-deiodinase, sodium-potassium ATPase ...
Xiaoqing, Zhang +3 more
openaire +2 more sources
Anion channels for amino acids in MDCK cells
American Journal of Physiology-Cell Physiology, 1992Large losses of amino acids by diffusion were previously observed in Madin-Darby canine kidney (MDCK) cells during volume regulation. Also, an outward rectifying anion channel was activated. Because this channel was not selective among anions, it was suggested that it could be permeable to amino acids.
U, Banderali, G, Roy
openaire +2 more sources
Polarized exocytosis in mdck cells is regulated by phosphorylation
Journal of Cell Science, 1995ABSTRACT Protein phosphorylation and dephosphorylation systems modulate many cellular activities and have recently been implicated in the in vitro transport of newly synthesized proteins. Here we show that polarized transport from the Golgi to the plasma membrane in intact MDCK cells is regulated by phosphorylation-dephosphorylation ...
C B, Brewer, M G, Roth
openaire +2 more sources
Measuring receptor recycling in polarized MDCK cells
2015Recycling of proteins such as channels, pumps, and receptors is critical for epithelial cell function. In this chapter we present a method to measure receptor recycling in polarized Madin-Darby canine kidney cells using an iodinated ligand. We describe a technique to iodinate transferrin (Tf), we discuss how (125)I-Tf can be used to label a cohort of ...
Luciana, Gallo, Gerard, Apodaca
openaire +2 more sources
Transfection Alters Ion Transport in MDCK Cells
Journal of Membrane Biology, 1996In the course of an investigation into the effect of Tamm-Horsfall protein (THP) on ion transport, we performed stable transfection of THP into MDCK cells using the SV40 or the cytomegalovirus (CMV) promoter. As controls, we transfected MDCK cells with an "empty" plasmid containing SV40 or CMV promoter but without THP cDNA.
D M, Kaji +5 more
openaire +2 more sources
Snail modulates cell metabolism in MDCK cells
Biochemical and Biophysical Research Communications, 2013Snail, a repressor of E-cadherin gene transcription, induces epithelial-to-mesenchymal transition and is involved in tumor progression. Snail also mediates resistance to cell death induced by serum depletion. By contrast, we observed that snail-expressing MDCK (MDCK/snail) cells undergo cell death at a higher rate than control (MDCK/neo) cells in low ...
Misako, Haraguchi +10 more
openaire +2 more sources
SNARE Protein Trafficking in Polarized MDCK Cells
Traffic, 2000A key feature of polarized epithelial cells is the ability to maintain the specific biochemical composition of the apical and basolateral plasma membrane domains. This polarity is generated and maintained by the continuous sorting of apical and basolateral components in the secretory and endocytic pathways.
M, Steegmaier +3 more
openaire +2 more sources