Results 161 to 170 of about 60,380 (203)

Snail modulates cell metabolism in MDCK cells

Biochemical and Biophysical Research Communications, 2013
Snail, a repressor of E-cadherin gene transcription, induces epithelial-to-mesenchymal transition and is involved in tumor progression. Snail also mediates resistance to cell death induced by serum depletion. By contrast, we observed that snail-expressing MDCK (MDCK/snail) cells undergo cell death at a higher rate than control (MDCK/neo) cells in low ...
Misako, Haraguchi, Hiroko P, Indo, Yasumasa, Iwasaki, Yoichiro, Iwashita, Tomoko, Fukushige, Hideyuki J, Majima, Kimiko, Izumo, Masahisa, Horiuchi, Takuro, Kanekura, Tatsuhiko, Furukawa, Masayuki, Ozawa   +10 more
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Regulation of Ripply1 expression in MDCK organoids

Biochemical and Biophysical Research Communications, 2015
Epithelial organs are made of a well-polarized monolayer of epithelial cells, and their morphology must be maintained for their proper function. To examine the genes that are specifically expressed in the late stages of cystogenesis and are involved in maintaining the morphology of the mature cysts, we performed a microarray analysis comparing the mRNA
Hisayoshi, Yoshizaki, Yuka, Kuwajima, Hiroshi, Minato, Etsuko, Kiyokawa   +3 more
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Transfection Alters Ion Transport in MDCK Cells

Journal of Membrane Biology, 1996
In the course of an investigation into the effect of Tamm-Horsfall protein (THP) on ion transport, we performed stable transfection of THP into MDCK cells using the SV40 or the cytomegalovirus (CMV) promoter. As controls, we transfected MDCK cells with an "empty" plasmid containing SV40 or CMV promoter but without THP cDNA.
D M, Kaji, J, Bates, J D, Goyzueta, K, Prasadan, H, Yu, S, Kumar   +5 more
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Measuring receptor recycling in polarized MDCK cells

2015
Recycling of proteins such as channels, pumps, and receptors is critical for epithelial cell function. In this chapter we present a method to measure receptor recycling in polarized Madin-Darby canine kidney cells using an iodinated ligand. We describe a technique to iodinate transferrin (Tf), we discuss how (125)I-Tf can be used to label a cohort of ...
Luciana, Gallo, Gerard, Apodaca
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SNARE Protein Trafficking in Polarized MDCK Cells

Traffic, 2000
A key feature of polarized epithelial cells is the ability to maintain the specific biochemical composition of the apical and basolateral plasma membrane domains. This polarity is generated and maintained by the continuous sorting of apical and basolateral components in the secretory and endocytic pathways.
M, Steegmaier, K C, Lee, R, Prekeris, R H, Scheller   +3 more
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Electrical Properties of MDCK Cells

1985
The electrical characterization of a transporting epithelium requires information three main aspects: (1) the overall electrical properties across the whole cell layer; (2) the transcellular permeation route; and (3) the paracellular pathway. In the present article we describe assumptions, techniques, efforts, and information collected on these aspects
L. González-Mariscal, L. Borboa, R. López-Vancell, G. Beaty, M. Cereijido   +4 more
openaire   +1 more source

Mineralocorticoid-induced membrane proteins in MDCK cells

Molecular and Cellular Endocrinology, 1982
The Madin-Darby canine kidney (MDCK) cell line, which exhibits properties indicative of a distal tubule origin, evidently binds and responds to mineralocorticoid hormones. We investigated the effects of aldosterone and deoxycorticosterone on protein synthesis in MDCK cells grown either in medium supplemented with serum or in a hormonally defined, serum-
M H, Cobb, C P, Yang, D M, Jefferson, E, Pasnikowski, W N, Scott   +4 more
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Ion Transport in MDCK Cells

1985
The use of monolayers of MDCK cells as a model system for natural transporting epithelia created the necessity of learning about their ion-translocating mechanisms. These mechanisms have been analyzed directly by measuring unidirectional fluxes of tracers, and indirectly through their consequences on electrical parameters and blister-forming activity ...
S. Fernández-Castelo, J. J. Bolívar, R. López-Vancell, G. Beaty, M. Cereijido   +4 more
openaire   +1 more source

Variability of functional characteristics of MDCK cells

American Journal of Physiology-Cell Physiology, 1986
We measured several functional parameters of MDCK cells cultured as monolayers in order to more fully characterize their ion transport properties. Most of the present studies were completed with five groups (A-E) of MDCK cells studied from passage 62 to 78.
R F, Husted, M J, Welsh, J B, Stokes
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Cyclic‐AMP deficient MDCK cells form tubules

Journal of Cellular Biochemistry, 1995
AbstractIt has known for many years that MDCK cells blister structures, termed domes. During an examination of the morphbology of a large number of MDCK clones, we found that two stable morphotypes exist in an MDCK cell population namely, dome‐forming and tubule‐forming clones. When maintained at high cell density, tubule‐forming clones displayed large
R J, Klebe, A, Grant, G, Grant, P, Ghosh
openaire   +2 more sources