Results 191 to 200 of about 41,345 (224)
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1985
The use of monolayers of MDCK cells as a model system for natural transporting epithelia created the necessity of learning about their ion-translocating mechanisms. These mechanisms have been analyzed directly by measuring unidirectional fluxes of tracers, and indirectly through their consequences on electrical parameters and blister-forming activity ...
S. Fernández-Castelo +4 more
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The use of monolayers of MDCK cells as a model system for natural transporting epithelia created the necessity of learning about their ion-translocating mechanisms. These mechanisms have been analyzed directly by measuring unidirectional fluxes of tracers, and indirectly through their consequences on electrical parameters and blister-forming activity ...
S. Fernández-Castelo +4 more
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1992
In polarized cells the plasma membrane is divided into apical and basolateral domains. Membrane is internalized from both these domains and the internalized components can follow several different routes, for example, they can be transported to the opposite domain of the cell (transcytosis), routed to lysosomes or recycled back to the same domain ...
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In polarized cells the plasma membrane is divided into apical and basolateral domains. Membrane is internalized from both these domains and the internalized components can follow several different routes, for example, they can be transported to the opposite domain of the cell (transcytosis), routed to lysosomes or recycled back to the same domain ...
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Influence of glucose on the toxicity of oxophenylarsine in MDCK cells
Archives of Toxicology, 1995Trivalent arsenicals like oxophenylarsine (PhAsO) inhibit cellular pyruvate dehydrogenase, thus leading to a drop of acetylCoA formation and a slow-down of the citric acid cycle. Glucose may protect cells from arsenic toxicity, because increased glycolysis may prevent fatal shortage of ATP.
B, Liebl +5 more
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Isolation and Characterization of MDCK-Subcultures
1983In order to obtain uniform populations derived from the MDCK cell line, the technique of centrifugal elutriation has been used. Seven cell types, which differ from each other by their size have been subcultured and characterized with regards to their morphology and stimulation of adenylate cyclase.
E. M. Giesen +5 more
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Polarized trafficking of thyrocyte proteins in MDCK cells
Molecular and Cellular Endocrinology, 2002Recent studies suggest striking similarities between polarized protein sorting in thyrocytes and MDCK epithelial cells, including apical trafficking of thyroglobulin (Tg), thyroid peroxidase, and aminopeptidase N; as well as basolateral targeting of heparan sulfate proteoglycans, thrombospondin 1 (TSP1), type 1 5'-deiodinase, sodium-potassium ATPase ...
Xiaoqing, Zhang +3 more
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Electrical Properties of MDCK Cells
1985The electrical characterization of a transporting epithelium requires information three main aspects: (1) the overall electrical properties across the whole cell layer; (2) the transcellular permeation route; and (3) the paracellular pathway. In the present article we describe assumptions, techniques, efforts, and information collected on these aspects
L. González-Mariscal +4 more
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Anion channels for amino acids in MDCK cells
American Journal of Physiology-Cell Physiology, 1992Large losses of amino acids by diffusion were previously observed in Madin-Darby canine kidney (MDCK) cells during volume regulation. Also, an outward rectifying anion channel was activated. Because this channel was not selective among anions, it was suggested that it could be permeable to amino acids.
U, Banderali, G, Roy
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Polarized exocytosis in mdck cells is regulated by phosphorylation
Journal of Cell Science, 1995ABSTRACT Protein phosphorylation and dephosphorylation systems modulate many cellular activities and have recently been implicated in the in vitro transport of newly synthesized proteins. Here we show that polarized transport from the Golgi to the plasma membrane in intact MDCK cells is regulated by phosphorylation-dephosphorylation ...
C B, Brewer, M G, Roth
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Methods for Establishing Rab Knockout MDCK Cells
2021The Rab family small GTPases are key regulators of intracellular membrane traffic that are conserved in all eukaryotic cells. Rabs are thought to regulate various steps of membrane traffic, including the budding, transport, tethering, docking, and fusion of vesicles or organelles.
Riko, Kinoshita +2 more
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Measuring receptor recycling in polarized MDCK cells
2015Recycling of proteins such as channels, pumps, and receptors is critical for epithelial cell function. In this chapter we present a method to measure receptor recycling in polarized Madin-Darby canine kidney cells using an iodinated ligand. We describe a technique to iodinate transferrin (Tf), we discuss how (125)I-Tf can be used to label a cohort of ...
Luciana, Gallo, Gerard, Apodaca
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