Results 181 to 190 of about 41,345 (224)

Comparison between MDCK and MDCK-SIAT1 cell lines as preferred host for cell culture-based influenza vaccine production

Biotechnology Letters, 2016
To evaluate MDCK and MDCK-SIAT1 cell lines for their ability to produce the yield of influenza virus in different Multiplicities of Infection. Results Yields obtained for influenza virus H1N1 grown in MDCK-SIAT1 cell was almost the same as MDCK; however,
Asghar Abdoli, Hoorieh Soleimanjahi, Abbas Jamali   +2 more
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Hydraulic Properties of MDCK Cell Epithelium

Journal of Membrane Biology, 1996
The water permeability of the apical and basolateral cell membranes and the compliance of the lateral intercellular spaces (LIS) of MDCK monolayers were measured on confluent cultures grown on permeable supports. Cell membrane water permeabilities were determined, using quantitative differential interference light microscopy, from the rate of cell ...
M M, Timbs, K R, Spring
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The Apical Membrane Glycocalyx of MDCK Cells

The Journal of Membrane Biology, 2000
The microenvironment near the apical membrane of MDCK cells was studied by quantitation of the fluorescence of wheat germ agglutinin attached to fluorescein (WGA). WGA was shown to bind to sialic acid residues attached to galactose at the alpha-2,3 position in the glycocalyx on the apical membrane.
O N, Kovbasnjuk, K R, Spring
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Apical membrane permeability of MDCK cells

American Journal of Physiology-Cell Physiology, 1996
The osmotic water permeability (Pf) and permeability to nonelectrolytes were determined for the apical membrane of clonal strain Madin-Darby canine kidney (MDCK) C12 cells cultured as cysts with the apical membrane facing the surrounding medium. Pf and solute permeabilities were calculated from the rate of volume change of cysts by digitizing images ...
R L, Rivers, J A, McAteer, J L, Clendenon, B A, Connors, A P, Evan, J C, Williams   +5 more
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Regulation of Ripply1 expression in MDCK organoids

Biochemical and Biophysical Research Communications, 2015
Epithelial organs are made of a well-polarized monolayer of epithelial cells, and their morphology must be maintained for their proper function. To examine the genes that are specifically expressed in the late stages of cystogenesis and are involved in maintaining the morphology of the mature cysts, we performed a microarray analysis comparing the mRNA
Hisayoshi, Yoshizaki, Yuka, Kuwajima, Hiroshi, Minato, Etsuko, Kiyokawa   +3 more
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Cytomatrix synthesis in MDCK epithelial cells

Journal of Cellular Physiology, 1990
AbstractDetailed information regarding the synthesis rates of individual protein components is important in understanding the assembly and dynamics of the cytoskeletal matrix of eukaryotic cells. As an approach to this topic, the dual isotope technique of Clark and Zak J. Biol.
J J, Mitchell, R B, Low, J L, Woodcock-Mitchell   +2 more
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Regulation of the MDCK Cell Tight Junction

Journal of Membrane Biology, 1998
The sodium flux across individual tight junctions (TJ) of low-resistance MDCK cell monolayers grown on glass coverslips was determined as a measure of paracellular permeability. Increases in perfusate glucose concentration from 5 to 25 mM decreased tight junction Na permeability. This permeability decrease was not specific as nonmetabolizable analogues
O N, Kovbasnjuk, U, Szmulowicz, K R, Spring   +2 more
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Validation of Human MDR1-MDCK and BCRP-MDCK Cell Lines to Improve the Prediction of Brain Penetration

Journal of Pharmaceutical Sciences, 2019
It is of great challenge to predict human brain penetration for substrates of multidrug resistance protein 1 (MDR1) and breast cancer resistance protein (BCRP), 2 major efflux transporters at blood-brain barrier. Thus, a physiologically based pharmacokinetic (PBPK) model with the incorporation of in vitro MDR1 and BCRP transporter function data and ...
Bo, Feng, Mark, West, Nandini C, Patel, Travis, Wager, Xinjun, Hou, Jillian, Johnson, Larry, Tremaine, Jennifer, Liras   +7 more
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Quantitative proteomic analysis of MDCK cell adhesion

Molecular Omics, 2020
Abstract MDCK cells are a key reagent in modern vaccine production. As MDCK cells are normally adherent, creation of suspension cells for vaccine production using genetic engineering approaches is highly desirable. However, little is known regarding the mechanisms and effectors underlying MDCK cell adhesion.
Xuanqing Ye, Jiamin Wang, Zilin Qiao, Di Yang, Jiao Wang, Ayimuguli Abudureyimu, Kun Yang, Yuping Feng, Zhongren Ma, Zhenbin Liu   +9 more
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Electrical properties of cultured epithelioid cells (MDCK)

The Journal of Membrane Biology, 1983
This is a study of the intracellular electrical potential, membrane resistance, and capacity of MDCK cells (epithelioid of renal origin) cultured in monolayers on a collagen couch. These monolayers have a transepithelial resistance of 256 +/- 12 (22) ohm cm2 (mean +/- standard error, and number of observations), and the cells have 61.6 +/- 6.3 (92) M ...
E, Stefani, M, Cereijido
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