Results 181 to 190 of about 57,512 (227)
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Methods for Establishing Rab Knockout MDCK Cells
2021The Rab family small GTPases are key regulators of intracellular membrane traffic that are conserved in all eukaryotic cells. Rabs are thought to regulate various steps of membrane traffic, including the budding, transport, tethering, docking, and fusion of vesicles or organelles.
Riko, Kinoshita +2 more
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Electrical properties of cultured epithelioid cells (MDCK)
The Journal of Membrane Biology, 1983This is a study of the intracellular electrical potential, membrane resistance, and capacity of MDCK cells (epithelioid of renal origin) cultured in monolayers on a collagen couch. These monolayers have a transepithelial resistance of 256 +/- 12 (22) ohm cm2 (mean +/- standard error, and number of observations), and the cells have 61.6 +/- 6.3 (92) M ...
E, Stefani, M, Cereijido
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The Apical Membrane Glycocalyx of MDCK Cells
The Journal of Membrane Biology, 2000The microenvironment near the apical membrane of MDCK cells was studied by quantitation of the fluorescence of wheat germ agglutinin attached to fluorescein (WGA). WGA was shown to bind to sialic acid residues attached to galactose at the alpha-2,3 position in the glycocalyx on the apical membrane.
O N, Kovbasnjuk, K R, Spring
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Transfection Alters Ion Transport in MDCK Cells
Journal of Membrane Biology, 1996In the course of an investigation into the effect of Tamm-Horsfall protein (THP) on ion transport, we performed stable transfection of THP into MDCK cells using the SV40 or the cytomegalovirus (CMV) promoter. As controls, we transfected MDCK cells with an "empty" plasmid containing SV40 or CMV promoter but without THP cDNA.
D M, Kaji +5 more
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SNARE Protein Trafficking in Polarized MDCK Cells
Traffic, 2000A key feature of polarized epithelial cells is the ability to maintain the specific biochemical composition of the apical and basolateral plasma membrane domains. This polarity is generated and maintained by the continuous sorting of apical and basolateral components in the secretory and endocytic pathways.
M, Steegmaier +3 more
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Mineralocorticoid-induced membrane proteins in MDCK cells
Molecular and Cellular Endocrinology, 1982The Madin-Darby canine kidney (MDCK) cell line, which exhibits properties indicative of a distal tubule origin, evidently binds and responds to mineralocorticoid hormones. We investigated the effects of aldosterone and deoxycorticosterone on protein synthesis in MDCK cells grown either in medium supplemented with serum or in a hormonally defined, serum-
M H, Cobb +4 more
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Measuring receptor recycling in polarized MDCK cells
2015Recycling of proteins such as channels, pumps, and receptors is critical for epithelial cell function. In this chapter we present a method to measure receptor recycling in polarized Madin-Darby canine kidney cells using an iodinated ligand. We describe a technique to iodinate transferrin (Tf), we discuss how (125)I-Tf can be used to label a cohort of ...
Luciana, Gallo, Gerard, Apodaca
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Variability of functional characteristics of MDCK cells
American Journal of Physiology-Cell Physiology, 1986We measured several functional parameters of MDCK cells cultured as monolayers in order to more fully characterize their ion transport properties. Most of the present studies were completed with five groups (A-E) of MDCK cells studied from passage 62 to 78.
R F, Husted, M J, Welsh, J B, Stokes
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Cyclic‐AMP deficient MDCK cells form tubules
Journal of Cellular Biochemistry, 1995AbstractIt has known for many years that MDCK cells blister structures, termed domes. During an examination of the morphbology of a large number of MDCK clones, we found that two stable morphotypes exist in an MDCK cell population namely, dome‐forming and tubule‐forming clones. When maintained at high cell density, tubule‐forming clones displayed large
R J, Klebe, A, Grant, G, Grant, P, Ghosh
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Electrical Properties of MDCK Cells
1985The electrical characterization of a transporting epithelium requires information three main aspects: (1) the overall electrical properties across the whole cell layer; (2) the transcellular permeation route; and (3) the paracellular pathway. In the present article we describe assumptions, techniques, efforts, and information collected on these aspects
L. González-Mariscal +4 more
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