Results 81 to 90 of about 16,963,019 (272)

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Toward a protocol for quantifying the greenhouse gas balance and identifying mitigation options in smallholder farming systems

Environmental Research Letters, 2013
Globally, agriculture is directly responsible for 14% of annual greenhouse gas (GHG) emissions and induces an additional 17% through land use change, mostly in developing countries (Vermeulen et al 2012).
T S Rosenstock, M C Rufino, K Butterbach-Bahl, E Wollenberg   +3 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Measures having equivalent effect to quantitative restrictions in imports in the meaning of Article 34 of the Treaty on the Functioning of the European Union

, 2017
Wydział Prawa i AdministracjiOgólnym celem pracy jest dokonanie rekonstrukcji zakazu stosowania środków o skutku równoważnym do ograniczeń ilościowych w przywozie (ŚOR) ustanowionego w art. 34 TFUE. Praca składa się z pięciu rozdziałów.
Malaga, Miłosz
core  

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

The equivalent effect of representative utterances in english-indonesian hygiene product advertisement

, 2017
Prafitri Kumalasari, The Equivalent Effect of Representative Utterances In English-Indonesian Hygiene Product Advertisement. A Thesis: English Department, Letters and Humanities Faculty, State Islamic University Syarif Hidayatullah Jakarta, 2017.In this ...
Muhammad Farkhan, Prafitri Kumalasari
core  

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Effectiveness of a Parent Training Intervention for Children with Comorbid Autism Spectrum Disorder and Behavioral Problems in Alexandria, Egypt

Journal of High Institute of Public Health
Background: Parents of children with autism spectrum disorder (ASD) experience several negative psychological outcomes that are compounded by the presence of children’s comorbid behavioral problems.
Sally S. Othman, Mervat W. Abu‑Nazel, Tarek E.I. Omar, Heba A. Abdelaziz, Zeinab N. Shata   +4 more
doaj   +1 more source

Farmer Field Schools for Improving Farming Practices and Farmer Outcomes: A Systematic Review

Campbell Systematic Reviews, 2014
This Campbell systematic review examines the effectiveness of farmer field schools in improving intermediate outcomes (such as knowledge and pesticide use) and final outcomes (such as agricultural yields, incomes and empowerment) in low‐ and middle ...
Hugh Waddington, Birte Snilstveit, Jorge Hombrados, Martina Vojtkova, Daniel Phillips, Philip Davies, Howard White   +6 more
doaj   +1 more source