Results 71 to 80 of about 12,181 (265)
Life, 2023 The multifarious internal workings of organisms are difficult to reconcile with a single feature defining a state of ‘being alive’. Indeed, definitions of life rely on emergent properties (growth, capacity to evolve, agency) only symptomatic of intrinsic functioning. Empirical studies demonstrate that biomolecules including ratcheting/rotating enzymes,
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Journal of Economic Theory, 2020 zbMATH Open Web Interface contents unavailable due to conflicting licenses.
Mackenzie, Andrew, Zhou, Yu
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FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
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Higher Education Without Commodification, Mechanization or Moralization [PDF]
Journal of Philosophical InvestigationsWhat is the ultimate end of higher education? Giambattista Vico, echoing Socrates’s Delphic oracle, claimed that the ultimate end of all education is self-knowledge.
Robert Hanna
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The mechanism of bacteriostasis [PDF]
Experimental Biology and Medicine, 1922 1. Between Gram-positive and Gram-negative organisms gentian violet exhibits the same type of selective activity whether the dye be added to the media on which the bacteria are planted unstained (extrinsic bacteriostasis), or the organisms be stained with it before being planted on plain agar (intrinsic bacteriostasis).
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FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
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Jurnal Riset PerkebunanCoconut (Cocos nucifera) plays a crucial role in agricultural economies, particularly in coconut-producing regions, where traditional de-husking methods are labor-intensive and time-consuming. This study focuses on the design, fabrication, and evaluation
Jide Olusegun Adedipe
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Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs
FEBS Open Bio, EarlyView.Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner +7 more
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