Results 141 to 150 of about 7,897 (182)

Molecular parameters in melittin immunogenicity

Journal of Peptide Science, 1997
Based on immunogenicity studies, two T-cell epitopes in melittin were found to be functional in guinea pigs, one being centrally located, the other one residing in the C-terminal chain. In Balb/c mice only the central epitope was found to be active. A human T-cell clone was found by T-cell proliferation studies to employ strictly the C-terminal chain ...
Curcio-Vonlanthen V, Schneider CH, Frutig K, Blaser K, Kalbacher H   +4 more
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Characterization of Melittin Effects in Synaptosomes

Neurochemical Research, 1997
The effects of melittin at increasing concentrations on: [3H]GABA release from mouse brain synaptosomes; on the radioactivity released from [3H]arachidonic acid labeled synaptosomal membranes; on synaptosomes ultrastructure and on the leakage of the cytoplasmic marker, lactate-dehydrogenase (LDH) was investigated.
L, González, V, Nekrassov, A, Castell, M, Sitges   +3 more
openaire   +2 more sources

Interaction of melittin with lipid membranes

Biochimica Et Biophysica Acta - Biomembranes, 1994
Interaction of melittin with lipid membranes was studied systematically with respect to its adsorption onto membranes, its effect on membrane leakage and fusion, and micellization at various melittin/lipid ratios. It was found that melittin has a strong affinity for adsorption onto lipid membranes.
Shinpei Ohki, Klaus Arnold, Dinesh K Sukumaran   +2 more
exaly   +3 more sources

Interaction of melittin with endotoxic lipid A

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1992
Several amphipathic and cationic substances are known to bind lipid A, the toxic component of bacterial lipopolysaccharides. In this report, we have characterized, by fluorescence methods, the interaction of melittin, an amphipathic and basic 26-residue polypeptide isolated from bee venom, with lipid A.
David, SA, Mathan, VI, Balaram, Padmanabhan   +2 more
openaire   +3 more sources

Membrane matrix disruption by melittin

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1972
Abstract Electron spin resonance spectroscopy was used to examine the interaction of the lytic polypeptide, melittin, with model membrane systems in order to determine the physical basis for its alterations of membrane properties. Sonically dispersed egg lecithin (phosphatidylcholine), phosphatidylserine, extracted Escherichia coli phospholipids, and
J C, Williams, R M, Bell
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Über Melittin, XI. Drei Melittine im Bienengift

Justus Liebigs Annalen der Chemie, 1972
AbstractAus Bienengift wird durch Gelfiltration und Chromatographie über einen Kationenaustauscher (CM‐Cellulose) Melittin (einheitlicher Gipfel) eluiert. Mittels Elektrophorese auf Cellogel (Cellulose‐acetat) wird das chromatographierte Melittin in mindestens 3 Komponenten aufgespalten. Die Methode ist einfach und gut reproduzierbar.
openaire   +1 more source

Melittin-induced fusion of acidic liposomes [PDF]

FEBS Letters, 1983
Melittin‐induced fusion of acidic liposomes. Fusion was observed in the electron‐microscope and assayed as intermixing of both liposomes' contents and membranes. The melittin concentrations required for fusion induction were in the μM range compared to over 10 mM Ca2+ required for a comparable effect.
G D Eytan
exaly   +3 more sources

The interaction of melittin with troponin C

Archives of Biochemistry and Biophysics, 1987
Melittin has been found to interact with troponin C with high affinity in the presence of Ca2+. The association constant approaches in magnitude that for melittin and calmodulin. The interaction results in a shift to lower wavelengths of the emission band of Trp-19 of melittin and in an increased shielding of Trp-19 from quenching.
R F, Steiner, L, Norris
openaire   +2 more sources

Mechanism of the conformational transition of melittin

Biochemistry, 1992
It is known that, while melittin at micromolar concentrations is unfolded under conditions of low ionic strength at neutral pH, it adopts a tetrameric alpha-helical structure under conditions of high ionic strength, at alkaline pH, or at high peptide concentrations.
Y, Goto, Y, Hagihara
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Melittin-regenerated purple membrane

Biochemistry (Moscow), 2009
We have investigated the character of melittin-regenerated purple membrane. Adding melittin to blue membrane causes the color transition and partial regeneration of the photocycle and the proton pump. The reconstitution of bacteriorhodopsin by melittin is proved to be charge-dependent.
Yue, Zhang, Tao, Su, Kun-Sheng, Hu
openaire   +2 more sources