Results 71 to 80 of about 23,537 (218)

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

open access: yesFEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow   +12 more
wiley   +1 more source

Study of surface tension of inorganic melts based on their chemical composition and temperature [PDF]

open access: yesMetalurgija, 2009
The paper deals with the investigation of selected physical – chemical properties of inorganic melts with respect to behavior of surfaces. An explorational approach was applied in order to acquire original data enabling extension of the existing ...
R. Dudek, L'. Dobrovský, J. Dobrovská
doaj  

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

open access: yesFEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Rheological Properties of Carbon Fiber and Carbon Black Filled Liquid Crystalline Polymer Melts

open access: yesApplied Rheology, 2001
The rheological properties of thermotropic liquid crystalline polymer (LCP) and its carbon fiber (CF) and carbon black (CB) filled composites in molten state were measured using a cone-plate rheometer. The measurements of the CF/LCP and CB/LCP melts were
Araki Katsuhiko   +2 more
doaj   +1 more source

Methods for calculating specifi c electrical conductivity of the LiF+NaF mixtures melt [PDF]

open access: yesИзвестия Саратовского университета. Новая серия: Серия Химия. Биология. Экология
The calculation of the specifi c electrical conductivity of lithium and sodium fl uorides mixtures melts is presented. The Iinterest in the LiF–NaF system melts is determined by their practical signifi cance.
Lavrenteva, Olga V.   +2 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

The capacity of hydrous fluids to transport and fractionate incompatible elements and metals within the Earth's mantle

open access: yesGeochemistry, Geophysics, Geosystems, 2014
Both silicate melts and aqueous fluids are thought to play critical roles in the chemical differentiation of the Earth's crust and mantle. Yet their relative effects are poorly constrained. We have addressed this issue by measuring partition coefficients
John Adam   +4 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Editorial: Advances in the study of fluids and melts under extreme conditions

open access: yesFrontiers in Earth Science, 2023
Simone Anzellini   +5 more
doaj   +1 more source

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