Results 61 to 70 of about 5,515,438 (264)

Lipidomics in research on yeast membrane lipid homeostasis [PDF]

, 2017
Mass spectrometry is increasingly used in research on membrane lipid homeostasis, both in analyses of the steady state lipidome at the level of molecular lipid species, and in pulse-chase approaches employing stable isotope-labeled lipid precursors ...
de Kroon, Anton I P M, Sub Membrane Biochemistry & Biophysics, Membrane Biochemistry and Biophysics   +2 more
core   +2 more sources

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Comprehensive profiling of the catalytic conformations of human Guanylate kinase

Nature Communications
Human guanylate kinase (GMPK) as the sole enzyme for GDP biosynthesis plays pivotal roles in antiviral prodrug activation and tumorigenesis. Despite its biological significance, the catalytic mechanism remains poorly understood.
Lei Wang, Zihuan Li, Yumi Xuan, Jingkun Qin, Shuju Li, Fumei Zhong, Yuexiao Song, Kanglong Yang, Mengqi Lv, Fudong Li, Zhang Jiahai, Yueyin Pan, Shouhong Guang, Yuzheng Zhao, Yunyu Shi, Xing Liu, Yingying Du, Jia Gao, Ke Ruan   +18 more
doaj   +1 more source

Mechanism of phosphate sensing and signaling revealed by rice SPX1-PHR2 complex structure

Nature Communications, 2021
SPX proteins sense phosphate levels in plant cells by binding to inositol polyphosphates (InsP) and suppressing the activity of PHR transcription factors. Here the authors show that when bound to InsP6, the rice SPX1 protein inhibits the activity of PHR2
Jia Zhou, Qinli Hu, Xinlong Xiao, Deqiang Yao, Shenghong Ge, Jin Ye, Haojie Li, Rujie Cai, Renyang Liu, Fangang Meng, Chao Wang, Jian-Kang Zhu, Mingguang Lei, Weiman Xing   +13 more
doaj   +1 more source

The heterodimeric amino acid transporters (HAT) of the SLC7/SLC3 family: A structure−function relationships and relevance to human pathology

FEBS Letters, EarlyView.
Heterodimeric amino acid transporters consist of SLC7 and SLC3 family proteins arranged in a conserved structural organization. They regulate nutrient transport across cell membranes, supporting essential cellular functions. These transporters also contribute to xenobiotic/drug uptake and distribution.
Mariafrancesca Scalise, Tiziano Mazza, Raffaella Scanga, Lara Console, Michele Galluccio, Cesare Indiveri   +5 more
wiley   +1 more source

The Membrane Steps of Bacterial Cell Wall Synthesis as Antibiotic Targets [PDF]

, 2016
Peptidoglycan is the major component of the cell envelope of virtually all bacteria. It has structural roles and acts as a selective sieve for molecules from the outer environment. Peptidoglycan synthesis is therefore one of the most important biogenesis
Eefjan Breukink, Liu, Yao, Breukink, Eefjan, Yao Liu, Sub Membrane Biochemistry & Biophysics, Membrane Biochemistry and Biophysics   +5 more
core   +1 more source

Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research

FEBS Letters, EarlyView.
What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley   +1 more source

The role of filamentous membraneless organelle in 3UTR-mediated Protein-Protein interfaces and localization: TIGER Domain

Supramolecular Materials
Cells contain numerous membrane-less (MLOs) and multiple membrane-bound organelles. Most of the MLOs have a spherical shape except for a few organelles (that have a filamentous appearance, e.g. FXR1 condensates, L-bodies, and TIS granules).
Kashif Rahim, Muhammad Umair, Ihtisham Ul Haq   +2 more
doaj   +1 more source

Rainbow Nucleus Charts Dynamic Interactome of Membrane-Less Organelles

Summary Membrane-less organelles (MLOs) perform diverse cellular functions, but how they interact to coordinate these processes remains poorly understood. Here we constructed a “Rainbow Nucleus” cell line to simultaneously visualize five nuclear MLOs using multi-spectral live-cell imaging.
Songtao Ye, Nadav Benhamou Goldfajn, Choon Leng So, Takanari Inoue, Danfeng Cai   +4 more
openaire   +2 more sources

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source