Results 101 to 110 of about 534,194 (307)

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

Double membrane formation in heterogeneous vesicles

Soft Matter, 2020
Coarse-grained models show double membrane formation from single-membrane, heterogeneous liposomes in the presence of small, hydrophobic molecules.
Dima Bolmatov, Jan-Michael Y. Carrillo, Bobby G. Sumpter, John Katsaras, Maxim O. Lavrentovich   +4 more
openaire   +3 more sources

Diphtheria toxin T-domain as a tool for inducing lipid vesicle fusion

Communications Chemistry
Membrane fusion is fundamental for many biological processes, including subcellular compartmentalization, cell growth, and exo- and endocytosis. It also plays a key role in the fabrication of artificial membrane structures, particularly vesicles. However,
Piotr Jasko, Maryame Bina, Daniel Frey, Cora-Ann Schoenenberger, Cornelia G. Palivan, Richard A. Kammerer   +5 more
doaj   +1 more source

Ciliary and Flagellar Membrane Vesicle (Ectosome) Purification

Bio-Protocol, 2014
Eukaryotic cilia/flagella are ideal organelles for the analysis of membrane trafficking, membrane assembly, and the functions of a variety of signal transduction molecules.
William Dentler
doaj   +1 more source

Active Membrane Fluctuations Studied by Micropipet Aspiration [PDF]

, 2001
We present a detailed analysis of the micropipet experiments recently reported in J-B. Manneville et al., Phys. Rev. Lett. 82, 4356--4359 (1999), including a derivation of the expected behaviour of the membrane tension as a function of the areal strain ...
A. N. Radionov, A. Royant, A.G. Petrov, D. Oesterhelt, E. Boroske, E. Evans, E. Evans, E. Pebay-Peyroula, F. Dumas, F. Faucon, F. Maingret, F. Maingret, G. Niggemann, H. J. Sass, H. Luecke, H. Luecke, J-B. Manneville, J. Heberle, J. Heberle, J. K. Lanyi, J. Prost, J. Prost, J. Prost, J.-B. Manneville, J.-L. Rigaud, M. Bloom, M. Bloom, M. I. Angelova, M. Jansen, M. Kummrow, M. M. Speretto, M. Seigneuret, M. Seigneuret, N. A. Dencher, O. G. Mouritsen, P. Bassereau, P. Méléard, Ph. Méléard, R. Henderson, R. Kwok, R. Lawaczeck, R. Lipowsky, S. Ramaswamy, S. Ramaswamy, S. Ramaswamy, S. Subramaniam, S. Subramaniam, T. Kouyama, U. Haupts, W. Helfrich, W. Kuhlbrandt, Y. Cao, Y. Kimura   +52 more
core   +2 more sources

Engineered extracellular vesicles enriched with the miR‐214/199a cluster enhance the efficacy of chemotherapy in ovarian cancer

Molecular Oncology, EarlyView.
Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang, Ayesha Alvero, Yi Qin, Mingjin Wang, Alexandra Fox, Yanfeng Li, Michael Millman, Amy Kemper, Gil Mor, Xian Shuang Liu, Michael Chopp, Zheng Gang Zhang, Yi Zhang   +12 more
wiley   +1 more source

Dynamic L-type CaV1.2 channel trafficking facilitates CaV1.2 clustering and cooperative gating. [PDF]

, 2018
L-type CaV1.2 channels are key regulators of gene expression, cell excitability and muscle contraction. CaV1.2 channels organize in clusters throughout the plasma membrane.
Brust-Mascher, Ingrid, Dixon, Rose E, Ghosh, Debapriya, Hell, Johannes W, Horne, Mary C, Navedo, Manuel F, Nieves-Cintrón, Madeline, Santana, Luis F, Tajada, Sendoa   +8 more
core   +2 more sources

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

Uptake of Helicobacter pylori Vesicles Is Facilitated by Clathrin-Dependent and Clathrin-Independent Endocytic Pathways

mBio, 2014
Bacteria shed a diverse set of outer membrane vesicles that function as transport vehicles to deliver effector molecules and virulence factors to host cells.
Annelie Olofsson, Lars Nygård Skalman, Ikenna Obi, Richard Lundmark, Anna Arnqvist   +4 more
doaj   +1 more source

Force balance and membrane shedding at the Red Blood Cell surface

, 2006
During the aging of the red-blood cell, or under conditions of extreme echinocytosis, membrane is shed from the cell plasma membrane in the form of nano-vesicles.
D. E. Discher, J. M. Werre, L. Backman, N. G. Van Kampen, Nir Gov, Pierre Sens, R. Bucki, R. E. Waugh, S. Levin, S.-C. Liu, S. P. Sutera, V. Bennett, W. H. Reinhart, Z. Tochner   +13 more
core   +1 more source