Results 311 to 320 of about 2,949,634 (335)
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Proteomic Analysis of Plasma Membrane Vesicles
Angewandte Chemie International Edition, 2009AbstractA simple and scalable method is presented for harvesting, purification, and on‐chip processing of mammalian plasma membrane vesicles (PMVs) optimized for downstream proteome analysis. After immobilization on a microfluidic flowcell of PMVs, the embedded membrane proteins are proteolytically digested, and the peptides harvested and analyzed by ...
Bauer, Brigitte +2 more
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Vesicle tethering complexes in membrane traffic
Journal of Cell Science, 2002Despite the recent progress in the field of membrane traffic, the question of how the specificity of membrane fusion is achieved has yet to be resolved. It has become apparent that the SNARE proteins, although central to the process of fusion, are often not the first point of contact between a vesicle and its target.
James R C, Whyte, Sean, Munro
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Membrane tensiometer for heavy giant vesicles
The European Physical Journal E, 2004One key parameter of giant-vesicles adhesion is their membrane tension, sigma. A theoretically simple but delicate way to impose (and measure) it is to use micropipette manipulation techniques. But usually, the vesicles are free and their tension is unknown, until an adhesion patch grows.
P-H, Puech, F, Brochard-Wyart
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Self‐Assembled Vesicles with Functionalized Membranes
Chemistry – A European Journal, 2012AbstractBiological membranes play a key role for the function of living organisms. Thus, many artificial systems have been designed to mimic natural cell membranes and their functions. A useful concept for the preparation of functional membranes is the embedding of synthetic amphiphiles into vesicular bilayers.
Benjamin, Gruber, Burkhard, König
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Membrane messengers: Extracellular vesicles
Science, 2016For many years, it seems, researchers have had only a limited understanding of cellular communication. That cells could talk to one another via secreted hormones and growth factors was well known. That they also communicate using elaborate vesicular messages written in nucleic acids, proteins, and lipids was not.
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Annexin V and vesicle membrane electroporation
European Biophysics Journal, 1997The method of membrane electroporation (ME) has been used as an analytical tool to quantify the effect of membrane curvature on transient electric pore formation, and on the adsorption of the protein annexin V (M(r)= 35,800) to the outer surface of unilamellar lipid vesicles (of radii 25 < or = a/nm < or = 200). Relaxation kinetic studies using optical
Tönsing, Katja +4 more
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Phospholipid exchange between bilayer membrane vesicles
Biochemistry, 1976The turbidity of lipid vesicles, freshly prepared by sonicating purified dimyristoyllecithin (DML) in dilute KCl solutions, was measured as a function of time at various temperatures. A sharp maximum in the rate of increase of turbidity is found just above the crystal:liquid-crystal phase transition temperature (Tm).
F J, Martin, R C, MacDonald
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Formation of Supported Membranes from Vesicles
Physical Review Letters, 2000Using a combination of the quartz crystal microbalance and surface plasmon resonance techniques, we have studied the spontaneous formation of supported lipid bilayers from small (approximately 25 nm) unilamellar vesicles. Together these experimental methods measure the amount of lipid adsorbed on the surface and the amount of water trapped by the lipid.
C A, Keller +3 more
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Polymerization in Polymerizable Vesicle Bilayer Membranes
Langmuir, 2000Polymerization reactions in lyotropic liquid crystalline phases have opened the way to the development of many novel materials. Inter alia, the two-dimensional self-assembly of amphiphiles in vesicle bilayers has attracted considerable interest as an ordered reaction medium.
Jung, M. +6 more
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Characterization and function of membrane vesicles in Gram-positive bacteria
Applied Microbiology and Biotechnology, 2021Yina Cao, H. Lin
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