Results 1 to 10 of about 36,213 (285)
Signal and noise in metabarcoding data.
PLoS ONE, 2023 Metabarcoding is a powerful molecular tool for simultaneously surveying hundreds to thousands of species from a single sample, underpinning microbiome and environmental DNA (eDNA) methods.
Zachary Gold +14 more
doaj +4 more sources
Improving plant DNA metabarcoding accuracy with ecological filters and Angiosperms353: Field and pollen microscopy validation [PDF]
Applications in Plant SciencesPremise Metabarcoding has become a successful tool for the identification of species in ecological assemblages. However, the usefulness of metabarcoding for identifying plant species has been hampered due to a lack of universal gene regions that work ...
Reed Clark Benkendorf +5 more
doaj +2 more sources
Diversity, 2022 DNA metabarcoding has proven to be an accessible, cost-effective, and non-invasive tool for dietary analysis of predators in situ. Although DNA metabarcoding provides numerous benefits in characterizing diet—such as detecting prey animals that are ...
Adam J. D. Marques +2 more
doaj +1 more source
The power of metabarcoding: Can we improve bioassessment and biodiversity surveys of stream macroinvertebrate communities? [PDF]
Metabarcoding and Metagenomics, 2021 Most stream bioassessment and biodiversity surveys are currently based on morphological identification of communities. However, DNA metabarcoding is emerging as a fast and cost-effective alternative for species identification. We compared both methods in
Jarno Turunen +5 more
doaj +3 more sources
VTAM: A robust pipeline for validating metabarcoding data using controls
Computational and Structural Biotechnology Journal, 2023 To obtain accurate estimates for biodiversity and ecological studies, metabarcoding studies should be carefully designed to minimize both false positive (FP) and false negative (FN) occurrences.
Aitor González +7 more
doaj +1 more source
Scientific Reports, 2022 The soil fauna of the tropics remains one of the least known components of the biosphere. Long-term monitoring of this fauna is hampered by the lack of taxonomic expertise and funding. These obstacles may potentially be lifted with DNA metabarcoding.
Yves Basset +23 more
doaj +1 more source
Ecological Indicators, 2021 Diatoms (Bacillariophyceae) are characterized by silicified cell walls that favor their long-term preservation in sediments, therefore widely used as bioindicators of present and past water conditions.
Wengang Kang +10 more
doaj +1 more source
Internal transcribed spacer primer evaluation for vascular plant metabarcoding [PDF]
Metabarcoding and Metagenomics, 2021 The unprecedented ongoing biodiversity decline necessitates scalable means of monitoring in order to fully understand the underlying causes. DNA metabarcoding has the potential to provide a powerful tool for accurate and rapid biodiversity monitoring ...
Andreas Kolter, Birgit Gemeinholzer
doaj +3 more sources
An Illumina metabarcoding pipeline for fungi [PDF]
Ecology and Evolution, 2014 AbstractHigh‐throughput metabarcoding studies on fungi and other eukaryotic microorganisms are rapidly becoming more frequent and more complex, requiring researchers to handle ever increasing amounts of raw sequence data. Here, we provide a flexible pipeline for pruning and analyzing fungal barcode (ITS rDNA) data generated as paired‐end reads on ...
Bálint, Miklós +4 more
openaire +4 more sources
Ecosphere, 2021 DNA metabarcoding has become a powerful technique for identifying the species present in a bulk environmental sample. The application of DNA metabarcoding to wildlife diet analysis is a particularly promising tool for exploring trophic interactions.
Aimee L. Massey +4 more
doaj +1 more source