Signal and noise in metabarcoding data.
PLoS ONE, 2023 Metabarcoding is a powerful molecular tool for simultaneously surveying hundreds to thousands of species from a single sample, underpinning microbiome and environmental DNA (eDNA) methods.
Zachary Gold +14 more
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Improving plant DNA metabarcoding accuracy with ecological filters and Angiosperms353: Field and pollen microscopy validation [PDF]
Applications in Plant SciencesPremise Metabarcoding has become a successful tool for the identification of species in ecological assemblages. However, the usefulness of metabarcoding for identifying plant species has been hampered due to a lack of universal gene regions that work ...
Reed Clark Benkendorf +5 more
doaj +2 more sources
Scientific Reports, 2023 Environmental DNA metabarcoding is increasingly implemented in biodiversity monitoring, including phytoplankton studies. Using 21 mock communities composed of seven unicellular diatom and dinoflagellate algae, assembled with different composition and ...
Nadia Marinchel +6 more
doaj +2 more sources
Parasites & VectorsBackground Gastrointestinal helminths are a very widespread group of intestinal parasites that can cause major health issues in their hosts, including severe illness or death.
Madison L. Miller +2 more
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Efficacy of metabarcoding for identification of fish eggs evaluated with mock communities [PDF]
Ecology and Evolution, 2020 There is urgent need for effective and efficient monitoring of marine fish populations. Monitoring eggs and larval fish may be more informative than that traditional fish surveys since ichthyoplankton surveys reveal the reproductive activities of fish ...
Elena M. Duke, Ronald S. Burton
doaj +2 more sources
Royal Society Open Science, 2015 We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned whole mitochondrial genome (mitogenome) sequences from 880 species, supplemented by partial mitogenome ...
Masaki Miya +11 more
openalex +2 more sources
Metabarcoding with Illumina and Oxford Nanopore Technologies provides complementary insights into tree seed mycobiota [PDF]
Environmental MicrobiomeBackground Culturing of fungi is labor-intensive and reveals limited diversity, while high-throughput sequencing of barcodes (i.e., metabarcoding) enables a simultaneous detection of fungi from multiple environmental samples.
Jana Mittelstrass +7 more
doaj +2 more sources
Unraveling Fish Community Diversity and Structure in the Yellow Sea: Evidence from Environmental DNA Metabarcoding and Bottom Trawling [PDF]
AnimalsThe use of environmental DNA (eDNA) metabarcoding to analyze fish species diversity across different aquatic ecosystems is well documented. Nonetheless, there is a gap in validating eDNA metabarcoding studies on the diversity and structure of fish ...
Jinyong Zhang +6 more
doaj +2 more sources
The evaluation of shotgun sequencing and rpoB metabarcoding for taxonomic profiling of bacterial communities [PDF]
BMC MicrobiologyBackground The importance of microbial community profiling has been increasingly recognized in biological and environmental research. While metabarcoding has been widely used for such analysis by targeting specific DNA sequences as markers, shotgun ...
Karine Durand +2 more
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The power of metabarcoding: Can we improve bioassessment and biodiversity surveys of stream macroinvertebrate communities? [PDF]
Metabarcoding and Metagenomics, 2021 Most stream bioassessment and biodiversity surveys are currently based on morphological identification of communities. However, DNA metabarcoding is emerging as a fast and cost-effective alternative for species identification. We compared both methods in
Jarno Turunen +5 more
doaj +3 more sources