Results 1 to 10 of about 11,811 (241)

Signal and noise in metabarcoding data.

PLoS ONE, 2023
Metabarcoding is a powerful molecular tool for simultaneously surveying hundreds to thousands of species from a single sample, underpinning microbiome and environmental DNA (eDNA) methods.
Zachary Gold, Andrew Olaf Shelton, Helen R Casendino, Joe Duprey, Ramón Gallego, Amy Van Cise, Mary Fisher, Alexander J Jensen, Erin D'Agnese, Elizabeth Andruszkiewicz Allan, Ana Ramón-Laca, Maya Garber-Yonts, Michaela Labare, Kim M Parsons, Ryan P Kelly   +14 more
doaj   +4 more sources

Metabarcoding and targeted barcoding can enhance Norwegian Continental Shelf macrofauna species inventories [PDF]

PeerJ
Metabarcoding of bulk community samples is a powerful tool to characterize marine softbottom macrofaunal communities, but high-quality taxonomic assignment is dependent on adequate sequence coverage in taxonomic databases.
Jon T. Hestetun, Anders Lanzén, Jon Anders Kongsrud, Tom Alvestad, Per-Otto Johansen, Thomas G. Dahlgren   +5 more
doaj   +3 more sources

Unveiling Vertebrate Biodiversity in Arid and Semi‐Arid Terrestrial Ecosystems Through eDNA Metabarcoding at Savanna Waterholes [PDF]

Evolutionary Applications
Applying environmental DNA (eDNA) metabarcoding to samples from waterholes and their surroundings offers a promising approach for monitoring terrestrial vertebrates in semi‐arid and arid ecosystems, such as the southern African savannas. However, minimal
Tamara Schenekar, Janine Baxter, Irmgard Sedlmayr, Julia Gladitsch, Sibusiso Mahlangu, Monica Mwale   +5 more
doaj   +2 more sources

The power of metabarcoding: Can we improve bioassessment and biodiversity surveys of stream macroinvertebrate communities? [PDF]

Metabarcoding and Metagenomics, 2021
Most stream bioassessment and biodiversity surveys are currently based on morphological identification of communities. However, DNA metabarcoding is emerging as a fast and cost-effective alternative for species identification. We compared both methods in
Jarno Turunen, Heikki Mykrä, Vasco Elbrecht, Dirk Steinke, Thomas Braukmann, Jukka Aroviita   +5 more
doaj   +3 more sources

COI Metabarcoding Provides Insights into the Highly Diverse Diet of a Generalist Salamander, Salamandra salamandra (Caudata: Salamandridae)

Diversity, 2022
DNA metabarcoding has proven to be an accessible, cost-effective, and non-invasive tool for dietary analysis of predators in situ. Although DNA metabarcoding provides numerous benefits in characterizing diet—such as detecting prey animals that are ...
Adam J. D. Marques, Vanessa A. Mata, Guillermo Velo-Antón   +2 more
doaj   +1 more source

VTAM: A robust pipeline for validating metabarcoding data using controls

Computational and Structural Biotechnology Journal, 2023
To obtain accurate estimates for biodiversity and ecological studies, metabarcoding studies should be carefully designed to minimize both false positive (FP) and false negative (FN) occurrences.
Aitor González, Vincent Dubut, Emmanuel Corse, Reda Mekdad, Thomas Dechatre, Ulysse Castet, Raphaël Hebert, Emese Meglécz   +7 more
doaj   +1 more source

Comparison of traditional and DNA metabarcoding samples for monitoring tropical soil arthropods (Formicidae, Collembola and Isoptera)

Scientific Reports, 2022
The soil fauna of the tropics remains one of the least known components of the biosphere. Long-term monitoring of this fauna is hampered by the lack of taxonomic expertise and funding. These obstacles may potentially be lifted with DNA metabarcoding.
Yves Basset, Mehrdad Hajibabaei, Michael T. G. Wright, Anakena M. Castillo, David A. Donoso, Simon T. Segar, Daniel Souto-Vilarós, Dina Y. Soliman, Tomas Roslin, M. Alex Smith, Greg P. A. Lamarre, Luis F. De León, Thibaud Decaëns, José G. Palacios-Vargas, Gabriela Castaño-Meneses, Rudolf H. Scheffrahn, Marleny Rivera, Filonila Perez, Ricardo Bobadilla, Yacksecari Lopez, José Alejandro Ramirez Silva, Maira Montejo Cruz, Angela Arango Galván, Héctor Barrios   +23 more
doaj   +1 more source

Diatom metabarcoding and microscopic analyses from sediment samples at Lake Nam Co, Tibet: The effect of sample-size and bioinformatics on the identified communities

Ecological Indicators, 2021
Diatoms (Bacillariophyceae) are characterized by silicified cell walls that favor their long-term preservation in sediments, therefore widely used as bioindicators of present and past water conditions.
Wengang Kang, Sten Anslan, Nicole Börner, Anja Schwarz, Robin Schmidt, Sven Künzel, Patrick Rioual, Paula Echeverría-Galindo, Miguel Vences, Junbo Wang, Antje Schwalb   +10 more
doaj   +1 more source

Internal transcribed spacer primer evaluation for vascular plant metabarcoding [PDF]

Metabarcoding and Metagenomics, 2021
The unprecedented ongoing biodiversity decline necessitates scalable means of monitoring in order to fully understand the underlying causes. DNA metabarcoding has the potential to provide a powerful tool for accurate and rapid biodiversity monitoring ...
Andreas Kolter, Birgit Gemeinholzer
doaj   +3 more sources

Comparison of mechanical sorting and DNA metabarcoding for diet analysis with fresh and degraded wolf scats

Ecosphere, 2021
DNA metabarcoding has become a powerful technique for identifying the species present in a bulk environmental sample. The application of DNA metabarcoding to wildlife diet analysis is a particularly promising tool for exploring trophic interactions.
Aimee L. Massey, Gretchen H. Roffler, Tessa Vermeul, Jennifer M. Allen, Taal Levi   +4 more
doaj   +1 more source