Results 31 to 40 of about 11,811 (241)

Scorpion metabarcoding v1

, 2022
The following steps include the protocols of the 1. Diet Regime 2. Dissection procedure 3. Beads extraction 4. Salt-out DNA extraction
openaire   +1 more source

Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding

Foods, 2021
The substitution of more appreciated animal species by animal species of lower commercial value is a common type of meat product adulteration. DNA metabarcoding, the combination of DNA barcoding with next-generation sequencing (NGS), plays an increasing ...
Laura Preckel, Claudia Brünen-Nieweler, Grégoire Denay, Henning Petersen, Margit Cichna-Markl, Stefanie Dobrovolny, Rupert Hochegger   +6 more
doaj   +1 more source

Metabarcoding with Illumina and Oxford Nanopore Technologies provides complementary insights into tree seed mycobiota

Environmental Microbiome
Background Culturing of fungi is labor-intensive and reveals limited diversity, while high-throughput sequencing of barcodes (i.e., metabarcoding) enables a simultaneous detection of fungi from multiple environmental samples.
Jana Mittelstrass, Renate Heinzelmann, René Eschen, Martin Hartmann, Quirin Kupper, Salome Schneider, Simone Prospero, Iva Franić   +7 more
doaj   +1 more source

Plant biodiversity assessment through pollen DNA metabarcoding in Natura 2000 habitats (Italian Alps)

Scientific Reports, 2021
Monitoring biodiversity is of increasing importance in natural ecosystems. Metabarcoding can be used as a powerful molecular tool to complement traditional biodiversity monitoring, as total environmental DNA can be analyzed from complex samples ...
Kleopatra Leontidou, Despoina Vokou, Anna Sandionigi, Antonia Bruno, Maria Lazarina, Johannes De Groeve, Mingai Li, Claudio Varotto, Matteo Girardi, Maurizio Casiraghi, Antonella Cristofori   +10 more
doaj   +1 more source

Comparison and validation of some ITS primer pairs useful for fungal metabarcoding studies.

PLoS ONE, 2014
Current metabarcoding studies aiming to characterize microbial communities generally rely on the amplification and sequencing of relatively short DNA regions.
Michiel Op De Beeck, Bart Lievens, Pieter Busschaert, Stéphan Declerck, Jaco Vangronsveld, Jan V Colpaert   +5 more
doaj   +1 more source

Comparative environmental RNA and DNA metabarcoding analysis of river algae and arthropods for ecological surveys and water quality assessment

Scientific Reports, 2022
Environmental DNA (eDNA) metabarcoding is widely used for species analysis, while the use of environmental RNA (eRNA) metabarcoding is more limited. We conducted comparative eDNA/eRNA metabarcoding of the algae and arthropods (aquatic insects) in water ...
Kaede Miyata, Yasuaki Inoue, Yuto Amano, Tohru Nishioka, Tomohisa Nagaike, Takamitsu Kawaguchi, Osamu Morita, Masayuki Yamane, Hiroshi Honda   +8 more
doaj   +1 more source

Comparison of environmental DNA metabarcoding and bottom trawling for detecting seasonal fish communities and habitat preference in a highly disturbed estuary

Ecological Indicators, 2023
Environmental DNA (eDNA) metabarcoding has been used to study fish diversity in various aquatic ecosystems. However, studies on fish community structure in estuarine ecosystems have not been well corroborated by eDNA metabarcoding, and comparisons with ...
Peiwen Jiang, Shuai Zhang, Shannan Xu, Pengli Xiong, Yiting Cao, Zuozhi Chen, Min Li   +6 more
doaj   +1 more source

Unearthing soil arthropod diversity through DNA metabarcoding

PeerJ, 2022
DNA metabarcoding has the potential to greatly advance understanding of soil biodiversity, but this approach has seen limited application for the most abundant and species-rich group of soil fauna–the arthropods. This study begins to address this gap by comparing information on species composition recovered from metabarcoding two types of bulk samples (
Monica R. Young, Paul D. N. Hebert
openaire   +3 more sources

Animal‐mediated seed dispersal: A review of study methods

Applications in Plant Sciences, EarlyView.
Abstract By dispersing seeds, animals provide ecological functions critical for the ecology, evolution, and conservation of plants. We review quantitative and empirical approaches and emerging technologies to quantify processes and patterns of animal‐mediated seed dispersal (zoochory) across its phases: from predispersal to postdispersal.
Noelle G. Beckman, Erin K. Kuprewicz, Binod Borah, James M. Bullock, Amanda S. Gallinat, Juan P. González‐Varo, Abir Jain, Jun Ying Lim, Carina I. Motta, Omer Nevo, Haldre S. Rogers   +10 more
wiley   +1 more source

Environmental DNA Metabarcoding Detects Predators at Higher Rates Than Electrofishing

Environmental DNA
There are numerous downsides and risks associated with electrofishing; hence, environmental DNA (eDNA) metabarcoding is becoming increasingly common in aquatic ecological studies.
Eric A. Bonk, Robert H. Hanner, Adrienne J. Bartlett, Gerald R. Tetreault   +3 more
doaj   +1 more source