Results 41 to 50 of about 11,811 (241)

Metabarcoding From Microbes to Mammals: Comprehensive Bioassessment on a Global Scale

Frontiers in Ecology and Evolution, 2020
Global biodiversity loss is unprecedented, and threats to existing biodiversity are growing. Given pervasive global change, a major challenge facing resource managers is a lack of scalable tools to rapidly and consistently measure Earth's biodiversity ...
Zacchaeus G. Compson, Beverly McClenaghan, Gregory A. C. Singer, Nicole A. Fahner, Mehrdad Hajibabaei, Mehrdad Hajibabaei   +5 more
doaj   +1 more source

The applicability of eDNA metabarcoding approaches for sessile benthic surveying in the Kimberley region, north‐western Australia

Environmental DNA, 2022
The application of environmental DNA technologies is a promising new approach to rapidly audit biodiversity across large‐scale, remote regions. Here, we examine the efficacy of a dual‐assay eDNA metabarcoding approach for sessile benthic bioassessments ...
Katrina M. West, Arne A. S. Adam, Nicole White, William D. Robbins, Daniel Barrow, Adrian Lane, Zoe T.Richards   +6 more
doaj   +1 more source

Metabarcoding a diverse arthropod mock community

Molecular Ecology Resources, 2019
Abstract Although DNA metabarcoding is an attractive approach for monitoring biodiversity, it is often difficult to detect all the species present in a bulk sample. In particular, sequence recovery for a given species depends on its biomass and mitome copy number as well as the primer set employed for PCR.
Thomas W. A. Braukmann, Natalia V. Ivanova, Sean W. J. Prosser, Vasco Elbrecht, Dirk Steinke, Sujeevan Ratnasingham, Jeremy R. de Waard, Jayme E. Sones, Evgeny V. Zakharov, Paul D. N. Hebert   +9 more
openaire   +2 more sources

A systematic review evaluating the performance of eDNA methods relative to conventional methods for biodiversity monitoring

Ecography, EarlyView.
The rapid adoption of environmental DNA (eDNA) methods has drastically changed biodiversity monitoring efforts. It is often claimed that eDNA methods are more sensitive and efficient than conventional biodiversity monitoring methods, but it is often unclear what metrics support this claim.
Nicholas J. Iacaruso, Olivia P. Reves, Sara J. Merkelz, Cassidy L. Waldrep, Mark A. Davis   +4 more
wiley   +1 more source

Unveiling Protist Composition and Diversity Patterns With eDNA Metabarcoding: Comparing Short- and Long-Read Approaches. [PDF]

Ecol Evol
Environmental DNA (eDNA) metabarcoding is widely used for biodiversity monitoring, but short‐read sequencing limits detailed taxonomic identification, especially for protists. In a comparison of short‐ and long‐read approaches in the Belgian North Sea, long‐read metabarcoding provided deeper taxonomic assignment under the applied workflows and improved
Skouroliakou DI, Dupont DWE, Vandenboer Y, D'Hont S, Sabbe K, Schön I.   +5 more
europepmc   +2 more sources

Observation Bias in Metabarcoding

Molecular Ecology Resources
ABSTRACTDNA metabarcoding is subject to observation bias associated with PCR and sequencing, which can result in observed read proportions differing from actual species proportions in the DNA extract. Here, we amplify and sequence a mock community of known composition containing marine fishes and cetaceans using four different primer sets and a variety
Megan R. Shaffer, Elizabeth Andruszkiewicz Allan, Amy M. Van Cise, Kim M. Parsons, Andrew Olaf Shelton, Ryan P. Kelly   +5 more
openaire   +2 more sources

Arbuscular mycorrhizal fungal community abundance, functions, and symbiotic interactions revealed by root metatranscriptomes

iMetaOmics, EarlyView.
Paradigm shift: PCR‐free methods reveal 6–15‐fold higher arbuscular mycorrhizal (AM) fungal abundance than metabarcoding, exposing systematic underestimation across decades of research. Predictive power: AM fungal abundance serves as a community‐level trait that predicts crop yield under drought conditions.
Peilin Chen, John W. Taylor, Cheng Gao
wiley   +1 more source

Improving plant DNA metabarcoding accuracy with ecological filters and Angiosperms353: Field and pollen microscopy validation

Applications in Plant Sciences
Premise Metabarcoding has become a successful tool for the identification of species in ecological assemblages. However, the usefulness of metabarcoding for identifying plant species has been hampered due to a lack of universal gene regions that work ...
Reed Clark Benkendorf, Emily J. Woodworth, Paul J. CaraDonna, Jane E. Ogilvie, Sophie Taddeo, Jeremie B. Fant   +5 more
doaj   +1 more source

Diversity and random forest models of oral microbiomes in periodontal health using publicly available data

Journal of Periodontology, EarlyView.
Abstract Background Evidence on the 16S metabarcoding of supragingival, subgingival, and salivary microbiomes in periodontal health remains limited. We aimed to analyze the diversity and potential of machine‐learning models of supragingival, subgingival, and salivary microbiomes in periodontal health. Methods A total of 848 samples (supragingival = 210;
Alba Regueira‐Iglesias, Berta Suárez‐Rodríguez, Triana Blanco‐Pintos, Alba Sánchez‐Barco, Marta Relvas, Carlos Balsa‐Castro, Inmaculada Tomás   +6 more
wiley   +1 more source

Laying Down the Foundations for Qualitative and Quantitative Freshwater Zooplankton Metabarcoding Surveys

Environmental DNA
Metabarcoding has been proven to be a highly effective tool for surveying biodiversity in aquatic ecosystems. Despite the recent explosion of metabarcoding studies, relatively few efforts have focused on freshwater zooplankton communities, even though ...
Jonas Bylemans, Didier Azam, Anne‐Laure Besnard, Simon Blanchet, Cecile Chardon, Gérard Lacroix, Leslie Laine, Stephen Mulero, Carine Puppo, Murielle Richard, Marine Vautier, Isabelle Domaizon   +11 more
doaj   +1 more source