Results 1 to 10 of about 359,868 (116)

Computational Modelling of Metabolic Burden and Substrate Toxicity in Escherichia coli Carrying a Synthetic Metabolic Pathway [PDF]

Microorganisms, 2019
In our previous work, we designed and implemented a synthetic metabolic pathway for 1,2,3-trichloropropane (TCP) biodegradation in Escherichia coli.
Martin Demko, Lukáš Chrást, Pavel Dvořák, Jiří Damborský, David Šafránek   +4 more
doaj   +2 more sources

Red Cell Distribution Width-Standard Deviation Is Associated with Cumulative Metabolic Burden but Not Independently with Metabolic Syndrome [PDF]

Medicina
Background and Objectives: Red cell distribution width (RDW) has been associated with adverse cardiometabolic outcomes; however, whether RDW—particularly RDW standard deviation (RDW-SD)—represents an independent determinant of metabolic syndrome (MetS ...
Kemal Ozan Lule, Nezihe Otay Lule, Mert Deniz Savcilioglu, Hamit Yildiz   +3 more
doaj   +2 more sources

Metabolomic Alterations Do Not Induce Metabolic Burden in the Industrial Yeast M2n[pBKD2-Pccbgl1]-C1 Engineered by Multiple δ-Integration of a Fungal β-Glucosidase Gene [PDF]

Frontiers in Bioengineering and Biotechnology, 2019
In the lignocellulosic yeast development, metabolic burden relates to redirection of resources from regular cellular activities toward the needs created by recombinant protein production. As a result, growth parameters may be greatly affected. Noteworthy,
Lorenzo Favaro, Lorenzo Cagnin, Laura Corte, Luca Roscini, Fabio De Pascale, Laura Treu, Stefano Campanaro, Marina Basaglia, Willem H. van Zyl, Sergio Casella, Gianluigi Cardinali, Gianluigi Cardinali   +11 more
doaj   +2 more sources

Intersecting Endocrine Pathways in Cardiomyopathy: The Role of Metabolic Burden in Structural Heart Disease [PDF]

Biomedicines
Background: Dilated cardiomyopathy (DCM) is a major contributor to heart failure-related morbidity and mortality. While type 2 diabetes mellitus (T2DM), obesity, and thyroid dysfunction are individually linked to cardiovascular disease, their combined ...
Ovidiu Țica, Mircea Ioan Șandor, Anca Huniadi, Cristian Daina, Sanda Monica Filip, Ilarie Brihan, Monica Sabău, Ioana Zaha, Otilia Țica   +8 more
doaj   +2 more sources

Lysinibacillus sphaericus III(3)7 and Plasmid Vector pMK4: New Challenges in Cloning Platforms

Microbiology Research, 2021
The acquisition and especially the maintenance of a plasmid usually brings a fitness cost that reduces the reproductive rate of the bacterial host; for strains like Lysinibacillus sphaericus III(3)7, which possesses important environmental properties ...
Diana C. León, Jenny Dussán
doaj   +1 more source

Central carbon metabolite profiling reveals vector-associated differences in the recombinant protein production host Escherichia coli BL21

Frontiers in Chemical Engineering, 2023
The Gram-negative bacterium Escherichia coli is the most widely used host for recombinant protein production, both as an industrial expression platform and as a model system at laboratory scale.
Laura García-Calvo, Divyata Vilas Rane, Nikalet Everson, Sigurd Tømmerberg Humlebrekk, Lise Femanger Mathiassen, Astfrid Helene Morka Mæhlum, Jostein Malmo, Per Bruheim   +7 more
doaj   +1 more source

Transcriptional network analysis identifies key elements governing the recombinant protein production provoked reprogramming of carbon and energy metabolism in Escherichia coli BL21 (DE3)

Engineering Reports, 2021
The impact of recombinant protein production on carbon and energy metabolism in Escherichia coli BL21 (DE3) was studied through transcriptome and proteome analysis of cells induced in carbon‐limited fed‐batch cultures during either fast or slow growth ...
Zhaopeng Li, Robert Geffers, Garima Jain, Frank Klawonn, Öznur Kökpinar, Manfred Nimtz, Wolfgang Schmidt‐Heck, Ursula Rinas   +7 more
doaj   +1 more source

Health Effects of Metabolic Risks in the United States From 1990 to 2019

Frontiers in Public Health, 2022
IntroductionMetabolic risks including high body mass index, high fasting plasma glucose, high low-density lipoprotein cholesterol, high systolic blood pressure, kidney dysfunction and low bone mineral density, contribute heavy burden to the US health ...
Ruifang Chen, Saeid Safiri, Masoud Behzadifar, Jude Dzevela Kong, Mohamed Sami Zguira, Nicola Luigi Bragazzi, Wen Zhong, Wen Zhong, Weiru Zhang, Weiru Zhang   +9 more
doaj   +1 more source

Delta-Integration of Single Gene Shapes the Whole Metabolomic Short-Term Response to Ethanol of Recombinant Saccharomyces cerevisiae Strains

Metabolites, 2020
In yeast engineering, metabolic burden is often linked to the reprogramming of resources from regular cellular activities to guarantee recombinant protein(s) production. Therefore, growth parameters can be significantly influenced.
Laura Corte, Luca Roscini, Debora Casagrande Pierantoni, Roberto Maria Pellegrino, Carla Emiliani, Marina Basaglia, Lorenzo Favaro, Sergio Casella, Gianluigi Cardinali   +8 more
doaj   +1 more source

Global burden of cardiovascular disease attributable to metabolic risk factors, 1990–2019: an analysis of observational data from a 2019 Global Burden of Disease study

BMJ Open, 2023
Objectives An up-to-date, detailed global analysis of the current status of the metabolic-attributed cardiovascular disease (CVD) burden has not been reported.
Zhang Jinhua, Shuyi Wu, Wenlin Xu, Chengfu Guan, Meina Lv, Shaojun Jiang   +5 more
doaj   +1 more source