Results 1 to 10 of about 508,176 (115)

Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli

Frontiers in Bioengineering and Biotechnology, 2021
Main reasons to produce recombinant proteins in the periplasm of E. coli rather than in its cytoplasm are to -i- enable disulfide bond formation, -ii- facilitate protein isolation, -iii- control the nature of the N-terminus of the mature protein, and -iv-
Alexandros Karyolaimos, Jan-Willem de Gier   +1 more
doaj   +3 more sources

The Goldilocks Approach: A Review of Employing Design of Experiments in Prokaryotic Recombinant Protein Production

Bioengineering, 2018
The production of high yields of soluble recombinant protein is one of the main objectives of protein biotechnology. Several factors, such as expression system, vector, host, media composition and induction conditions can influence recombinant protein ...
Albert Uhoraningoga, Gemma K. Kinsella, Gary T. Henehan, Barry J. Ryan   +3 more
doaj   +3 more sources

Proteomics and metabolic burden analysis to understand the impact of recombinant protein production in E. coli [PDF]

Scientific Reports
The impact of recombinant protein production (RPP) on host cells and the metabolic burden associated with it undermine the efficiency of the production system.
Girish H. Rajacharya, Ashima Sharma, Syed Shams Yazdani   +2 more
doaj   +2 more sources

Optimization of a novel expression system for recombinant protein production in CHO cells [PDF]

Scientific Reports
Chinese hamster ovary (CHO) cells are common mammalian cell lines for expressing recombinant proteins, yet the expression level of recombinant proteins is still hindered.
Junhe Zhang, Chenyang Du, Yue Pan, Zhan Zhang, Ruoyuan Feng, Mengyao Ma, Tianyun Wang   +6 more
doaj   +2 more sources

Protection and Immune Responses Elicited by rSAG1-PLGA Nanoparticles in C57BL/6 Against Toxoplasma gondii [PDF]

Journal of Medical Microbiology and Infectious Diseases, 2021
Introduction: This study aimed to evaluate rSAG1-PLGA efficacy as a particulate vaccine in conferring protection against Toxoplasma gondii infection in C57BL/6 mice.
Mojgan Allahyari, Samira amiri, Alireza Vatanara, Majid Golkar   +3 more
doaj   +2 more sources

Central carbon metabolite profiling reveals vector-associated differences in the recombinant protein production host Escherichia coli BL21

Frontiers in Chemical Engineering, 2023
The Gram-negative bacterium Escherichia coli is the most widely used host for recombinant protein production, both as an industrial expression platform and as a model system at laboratory scale.
Laura García-Calvo, Divyata Vilas Rane, Nikalet Everson, Sigurd Tømmerberg Humlebrekk, Lise Femanger Mathiassen, Astfrid Helene Morka Mæhlum, Jostein Malmo, Per Bruheim   +7 more
doaj   +1 more source

Omics Analysis Reveals the Mechanism of Enhanced Recombinant Protein Production Under Simulated Microgravity

Frontiers in Bioengineering and Biotechnology, 2020
Simulated microgravity (SMG) is regarded as a suitable environment to produce recombinant proteins. This study showed that β-glucuronidase expressing Escherichia coli had higher productivity of recombinant protein and higher plasmid copy number under SMG
Jie Huangfu, Jie Huangfu, Hye Su Kim, Ke Xu, Ke Xu, Xiaoyu Ning, Lei Qin, Jun Li, Chun Li   +8 more
doaj   +1 more source

Engineering a novel endopeptidase based on SARS 3CLpro

BioTechniques, 2009
A 3C-like protease (3CLpro) from the severe acute respiratory syndrome–coronavirus (SARS-CoV) is required for viral replication, cleaving the replicase polyproteins at 11 sites with the conserved Gln↓(Ser, Ala, Gly) sequences. In this study, we developed
Chih-Jung Kuo, Yan-Ping Shih, Daphne Kan, Po-Huang Liang   +3 more
doaj   +1 more source

Streptomycetes: Attractive Hosts for Recombinant Protein Production

Frontiers in Microbiology, 2020
Enzymes are increasingly applied as biocatalysts for fulfilling industrial needs in a variety of applications and there is a bursting of interest for novel therapeutic proteins.
Francesca Berini, Flavia Marinelli, Elisa Binda   +2 more
doaj   +1 more source

Engineering Saccharomyces cerevisiae for the production and secretion of Affibody molecules

Microbial Cell Factories, 2022
Background Affibody molecules are synthetic peptides with a variety of therapeutic and diagnostic applications. To date, Affibody molecules have mainly been produced by the bacterial production host Escherichia coli.
Veronica Gast, Anna Sandegren, Finn Dunås, Siri Ekblad, Rezan Güler, Staffan Thorén, Marta Tous Mohedano, Mikael Molin, Martin K. M. Engqvist, Verena Siewers   +9 more
doaj   +1 more source