Results 21 to 30 of about 508,275 (214)

Improvement of solubility and yield of recombinant protein expression in E. coli using a two-step system

Research in Pharmaceutical Sciences, 2019
Overexpression of recombinant proteins in Escherichia coli results in inclusion body formation, and consequently decreased production yield and increased production cost.
Tahereh Sadeghian-Rizi, Azade Ebrahimi, Fatemeh Moazzen, Hesam Yousefian, Ali Jahanian-Najafabadi   +4 more
doaj   +1 more source

Разработка кандидатной субстанции рекомбинантного белка CRM197

Эпидемиология и вакцинопрофилактика, 2016
CRM197 is a non-toxic mutant of diphtheria toxin having a single amino acid substitution of a glycine for a glutamic acid in position 52. Being naturally nontoxic, CRM197 is a promising adjuvant and ideal carrier protein for conjugate vaccines. Typically,
E. G. Bogomolova, O. A. Dobrovol'skaya, A. A. Mirovskaya, R. I. Al-Shehadat, E. A. Fedorova, I. V. Dukhovlinov, A. S. Simbirtsev   +6 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Targeted cancer treatment using a novel EGFR-specific Fc-fusion peptide based on GE11 peptide

Scientific Reports
Fc-fusion peptides, also known as peptibodies, are a promising new category of targeted therapeutics that offer alternatives to monoclonal antibodies (mAbs) for cancer treatment.
Malihe Hallaji, Mojgan Allahyari, Ladan Teimoori-Toolabi, Setayesh Yasami-Khiabani, Majid Golkar, Pezhman Fard-Esfahani   +5 more
doaj   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Scaling-up fermentation of Escherichia coli for production of recombinant P64k protein from Neisseria meningitidis

Electronic Journal of Biotechnology, 2018
Background: P64k is a Neisseria meningitidis high molecular weight protein present in meningococcal vaccine preparations. The lpdA gene, which encodes for this protein, was cloned in Escherichia coli and the P64k recombinant protein was expressed in E ...
Raúl Espinosa Pérez, José García Suárez, Emilio Narciandi Diaz, Ricardo Silva Rodríguez, Evelin Caballero Menéndez, Héctor Díaz Balaguer, Alexis Musacchio Lasa   +6 more
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

FcIgG-GE11-Melittin as a novel EGFR targeted peptibody with potent cytotoxic activity against cancer cells

Scientific Reports
The overexpression of epidermal growth factor receptor (EGFR) in various cancer types makes it an attractive target for therapeutic intervention. In this study, we designed a novel peptibody, FcIgG-GE11-Melittin, by fusing Melittin, a cytotoxic peptide ...
Malihe Hallaji, Shima Fayaz, Mojgan Allahyari, Majid Golkar, Kamran Pooshang-Bagheri, Pezhman Fard-Esfahani   +5 more
doaj   +1 more source

Presence of protein production enhancers results in significantly higher methanol-induced protein production in Pichia pastoris

Microbial Cell Factories, 2018
Background The yeast Komagataella phaffii, better known as Pichia pastoris, is a commonly used host for recombinant protein production. Here expression vectors are reported that address the different steps of the transcription–translation–secretion ...
Loknath Gidijala, Stefan Uthoff, Sebastiaan J. van Kampen, Alexander Steinbüchel, Raymond M. D. Verhaert   +4 more
doaj   +1 more source