Results 11 to 20 of about 508,275 (214)
Frontiers in Bioengineering and Biotechnology, 2020 Recently, we engineered a tunable rhamnose promoter-based setup for the production of recombinant proteins in E. coli. This setup enabled us to show that being able to precisely set the production rate of a secretory recombinant protein is critical to ...
Alexandros Karyolaimos +8 more
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Improved Yield of Recombinant Protein via Flagella Regulator Deletion in Escherichia coli
Frontiers in Microbiology, 2021 Protein production requires a significant amount of intracellular energy. Eliminating the flagella has been proposed to help Escherichia coli improve protein production by reducing energy consumption. In this study, the gene encoding a subunit of FlhC, a
Jae-Ho Han, Sang Taek Jung, Min-Kyu Oh
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Microbiology Spectrum, 2022 Flaviviruses are important human pathogens worldwide. Diagnostic testing for these viruses is difficult because many of the pathogens require specialized biocontainment.
Jordan A. Powers +9 more
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Applied Sciences, 2021 The enhancement of recombinant therapeutic protein production in mammalian cell culture has been regarded as an important issue in the biopharmaceutical industry.
Hyeonjin Cha, Ju-Hyun Park
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Frontiers in Bioengineering and Biotechnology, 2016 Agrobacterium-mediated transient expression systems enable plants to produce a wide range of recombinant proteins on a rapid timescale. To achieve economically feasible upstream production and downstream processing, two yield parameters should be ...
Naomichi eFujiuchi +3 more
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Journal of the Indonesian Tropical Animal Agriculture, 2020 This research aimed to express and purify the recombinant Fusion (F) protein of Newcastle Disease Virus (NDV) from a local isolate in Galur, Kulon Progo, Indonesia (0663/04/2013) from recombinant vector plasmid pBT7-N-His F, and to study the antibody ...
R.W. Astuti, N. Wijayanti, A. Haryanto
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Scientific Reports, 2022 The silkworm, Bombyx mori, is an attractive host for recombinant protein production due to its high expression efficiency, quality, and quantity. Two expression systems have been widely used for recombinant protein production in B.
Hiroyuki Kajiura +7 more
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Microbial Cell Factories, 2018 Background The production of recombinant proteins with proper conformation, appropriate post-translational modifications in an easily scalable and cost-effective system is challenging.
Susheel K. Singh +5 more
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Microbiology Spectrum, 2023 The autotransporter protein secretion system has been used previously to target the secretion of heterologous proteins to the bacterial cell surface and the extracellular milieu at the laboratory scale.
Irene Beriotto +12 more
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Microbial Cell Factories, 2019 Background Escherichia coli (E. coli) is a bacteria that is widely employed in many industries for the production of high interest bio-products such as recombinant proteins. Nevertheless, the use of E.
Gema Lozano Terol +4 more
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