Results 111 to 120 of about 2,439,575 (338)

Global organization of metabolic fluxes in the bacterium, Escherichia coli

, 2004
Cellular metabolism, the integrated interconversion of thousands of metabolic substrates through enzyme-catalyzed biochemical reactions, is the most investigated complex intercellular web of molecular interactions.
A Goldbeter, A Wagner, A-L Barabási, A.-L. Barabási, B. Kovács, D Segre, DM Wolf, E Fischer, E Ravasz, E. Almaas, F Canonaco, FR Blattner, H Jeong, HW Ma, J Stelling, JS Edwards, JS Edwards, JS Edwards, KI Goh, L Lovász, LH Hartwell, M Barthelemy, M Emmerling, MA Savageau, P Holme, R Heinrich, RL Smith, RU Ibarra, S Schuster, SY Gerdes, T Dandekar, T. Vicsek, U Sauer, Z. N. Oltvai   +33 more
core   +1 more source

Kinetic flux profiling for quantitation of cellular metabolic fluxes [PDF]

Nature Protocols, 2008
This protocol enables quantitation of metabolic fluxes in cultured cells. Measurements are based on the kinetics of cellular incorporation of stable isotope from nutrient into downstream metabolites. At multiple time points, after cells are rapidly switched from unlabeled to isotope-labeled nutrient, metabolism is quenched, metabolites are extracted ...
Jie, Yuan, Bryson D, Bennett, Joshua D, Rabinowitz   +2 more
openaire   +2 more sources

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source

Maintaining maximal metabolic flux by gene expression control

bioRxiv, 2017
Many evolutionarily successful bacteria attain high growth rates across growth-permissive conditions. They express metabolic networks that synthesise all cellular components at a high rate. Metabolic reaction rates are bounded by the concentration of the
R. Planqué, J. Hulshof, B. Teusink, Johannes C Hendriks, F. Bruggeman   +4 more
semanticscholar   +1 more source

PYCR1 inhibition in bone marrow stromal cells enhances bortezomib sensitivity in multiple myeloma cells by altering their metabolism

Molecular Oncology, EarlyView.
This study investigated how PYCR1 inhibition in bone marrow stromal cells (BMSCs) indirectly affects multiple myeloma (MM) cell metabolism and viability. Culturing MM cells in conditioned medium from PYCR1‐silenced BMSCs impaired oxidative phosphorylation and increased sensitivity to bortezomib.
Inge Oudaert, Lauren van den Broecke, Osman Aksoy, Judith Lind, Sonia Vallet, Arne Van der Vreken, Gamze Ates, Ann Massie, Ken Maes, Kim De Veirman, Elke De Bruyne, Karin Vanderkerken, Klaus Podar, Eline Menu   +13 more
wiley   +1 more source

Flux cost functions and the choice of metabolic fluxes

, 2018
Metabolic fluxes in cells are governed by physical, biochemical, physiological, and economic principles. Cells may show "economical" behaviour, trading metabolic performance against the costly side-effects of high enzyme or metabolite concentrations ...
Liebermeister, Wolfram
core  

Inhibition of CDK9 enhances AML cell death induced by combined venetoclax and azacitidine

Molecular Oncology, EarlyView.
The CDK9 inhibitor AZD4573 downregulates c‐MYC and MCL‐1 to induce death of cytarabine (AraC)‐resistant AML cells. This enhances VEN + AZA‐induced cell death significantly more than any combination of two of the three drugs in AraC‐resistant AML cells.
Shuangshuang Wu, Jianlei Zhao, Aaban Asfar Azmi, Avanti Gupte, Jenna Thibodeau, Shuang Liu, Jinli Yang, Guan Wang, Holly Edwards, Lisa A. Polin, Juiwanna Kushner, Sijana H. Dzinic, Kathryn White, Julie Boerner, Maik Hüttemann, Jay Yang, Yue Wang, Jeffrey W. Taub, Yubin Ge   +18 more
wiley   +1 more source

Evolution and regulation of nitrogen flux through compartmentalized metabolic networks in a marine diatom

Nature Communications, 2019
Diatoms outcompete other phytoplankton for nitrate, yet little is known about the mechanisms underpinning this ability. Genomes and genome-enabled studies have shown that diatoms possess unique features of nitrogen metabolism however, the implications ...
Sarah R. Smith, C. Dupont, James K. McCarthy, Jared T. Broddrick, M. Oborník, A. Horák, Zoltán Füssy, Jaromír Cihlář, Sabrina Kleessen, Hong Zheng, J. McCrow, K. Hixson, W. Araújo, A. Nunes‐Nesi, A. Fernie, Z. Nikoloski, B. Palsson, A. Allen   +17 more
semanticscholar   +1 more source

A synthetic benzoxazine dimer derivative targets c‐Myc to inhibit colorectal cancer progression

Molecular Oncology, EarlyView.
Benzoxazine dimer derivatives bind to the bHLH‐LZ region of c‐Myc, disrupting c‐Myc/MAX complexes, which are evaluated from SAR analysis. This increases ubiquitination and reduces cellular c‐Myc. Impairing DNA repair mechanisms is shown through proteomic analysis.
Nicharat Sriratanasak, Bodee Nutho, Worawat Wattanathana, Narumon Phaonakrop, Bunnatut Panasawatwong, Katharina Erlenbach‐Wuensch, Sittiruk Roytrakul, Regine Schneider‐Stock, Pithi Chanvorachote   +8 more
wiley   +1 more source

Effect of Drought on the Methylerythritol 4-Phosphate (MEP) Pathway in the Isoprene Emitting Conifer Picea glauca

Frontiers in Plant Science, 2020
The methylerythritol 4-phosphate (MEP) pathway of isoprenoid biosynthesis produces chlorophyll side chains and compounds that function in resistance to abiotic stresses, including carotenoids, and isoprene.
Erica Perreca, Johann Rohwer, Diego González-Cabanelas, Francesco Loreto, Axel Schmidt, Jonathan Gershenzon, Louwrance Peter Wright   +6 more
doaj   +1 more source