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Metabolic Fluxes and Metabolic Engineering
Metabolic Engineering, 1999Metabolic engineering is the directed improvement of cellular properties through the modification of specific biochemical reactions or the introduction of new ones, with the use of recombinant DNA technology. As such, metabolic engineering emphasizes metabolic pathway integration and relies on metabolic fluxes as determinants of cell physiology and ...
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Metabolic Engineering, 2001
Metabolic flux analysis using 13C-labeled substrates has become an important tool in metabolic engineering. It allows the detailed quantification of all intracellular fluxes in the central metabolism of a microorganism. The method has strongly evolved in recent years by the introduction of new experimental procedures, measurement techniques, and ...
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Metabolic flux analysis using 13C-labeled substrates has become an important tool in metabolic engineering. It allows the detailed quantification of all intracellular fluxes in the central metabolism of a microorganism. The method has strongly evolved in recent years by the introduction of new experimental procedures, measurement techniques, and ...
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Determining Compartment-Specific Metabolic Fluxes
2018In this chapter, we present an experimental protocol for the targeted metabolic profiling of full cells and mitochondria in selectively permeabilized cells. Mitochondria of adherent cell cultures are made accessible by the addition of digitonin-a compound that selectively permeabilizes the cytosolic membrane without affecting mitochondrial integrity ...
Nonnenmacher, Y, Palorini, R, Hiller, K
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Spectroscopic measurements of metabolic fluxes
Nature Biomedical Engineering, 2020The conversion of a deuterated substrate into cellular metabolites in vivo can be quantified via decreases in the proton nuclear magnetic resonance signals of these metabolites.
Peter C M, van Zijl, Kevin M, Brindle
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Metabolic Fluxes in Cancer Metabolism
2015Since Otto Warburg’s work almost a century ago, we have known that cancer cells undergo a metabolic transformation that makes them convert the majority of their glucose carbon into lactate. In the past decade it has become clear that this metabolic signature of cancer cells is controlled by the activation of oncogenes and the loss of tumor suppressors,
Ulrich L. Günther +6 more
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Flux modules in metabolic networks
Journal of Mathematical Biology, 2013The huge number of elementary flux modes in genome-scale metabolic networks makes analysis based on elementary flux modes intrinsically difficult. However, it has been shown that the elementary flux modes with optimal yield often contain highly redundant information. The set of optimal-yield elementary flux modes can be compressed using modules.
Müller, Arne C., Bockmayr, Alexander
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Metabolic flux analysis in eukaryotes
Current Opinion in Biotechnology, 2010Metabolic flux analysis (MFA) represents a powerful tool for systems biology research on eukaryotic cells. This review describes recent advances, the challenges as well as applications of metabolic flux analysis comprising fungi, mammalian cells and plants.
Jens, Niklas +2 more
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Metabolic flux control in glycosylation
Current Opinion in Structural Biology, 2016Glycosylation is a common post-translational protein modification, in which glycans are built onto proteins through the sequential addition of monosaccharide units, in reactions catalysed by glycosyltransferases. Glycosylation influences the physicochemical and biological properties of proteins, with subsequent effects on subcellular and extracellular ...
Andrew G, McDonald +2 more
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Metabolic Flux Analysis and Visualization
Journal of Proteome Research, 2011One of the ultimate goals of systems biology research is to obtain a comprehensive understanding of the control mechanisms of complex cellular metabolisms. Metabolic Flux Analysis (MFA) is a important method for the quantitative estimation of intracellular metabolic flows through metabolic pathways and the elucidation of cellular physiology.
Yoshihiro, Toya +3 more
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Genetically constrained metabolic flux analysis
Metabolic Engineering, 2005Significant progress has been made in using existing metabolic databases to estimate metabolic fluxes. Traditional metabolic flux analysis generally starts with a predetermined metabolic network. This approach has been employed successfully to analyze the behaviors of recombinant strains by manually adding or removing the corresponding pathway(s) in ...
Steven J, Cox +3 more
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