Results 71 to 80 of about 2,439,575 (338)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Molecular bases of circadian magnesium rhythms across eukaryotes

FEBS Letters, EarlyView.
Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley   +1 more source

Comprehensive Flux Modeling of Chlamydia trachomatis Proteome and qRT-PCR Data Indicate Biphasic Metabolic Differences Between Elementary Bodies and Reticulate Bodies During Infection

Frontiers in Microbiology, 2019
Metabolic adaptation to the host cell is important for obligate intracellular pathogens such as Chlamydia trachomatis (Ct). Here we infer the flux differences for Ct from proteome and qRT-PCR data by comprehensive pathway modeling.
Manli Yang, Karthika Rajeeve, Karthika Rajeeve, Thomas Rudel, Thomas Dandekar, Thomas Dandekar   +5 more
doaj   +1 more source

Productivity enhancement in L-lysine fermentation using oxygen-enhanced bioreactor and oxygen vector

Frontiers in Bioengineering and Biotechnology, 2023
Introduction: L-lysine is a bulk product. In industrial production using high-biomass fermentation, the high density of bacteria and the intensity of production require sufficient cellular respiratory metabolism for support.
Jinduo Wang, Jinduo Wang, Shuo Wang, Shuo Wang, Siyu Zhao, Siyu Zhao, Pengjie Sun, Pengjie Sun, Zhen Zhang, Zhen Zhang, Qingyang Xu, Qingyang Xu   +11 more
doaj   +1 more source

Elasticity sampling links thermodynamics to metabolic control

, 2013
Metabolic networks can be turned into kinetic models in a predefined steady state by sampling the reaction elasticities in this state. Elasticities for many reversible rate laws can be computed from the reaction Gibbs free energies, which are determined ...
Liebermeister, Wolfram
core   +2 more sources

MetaboTools: A comprehensive toolbox for analysis of genome-scale metabolic models [PDF]

, 2016
Metabolomic data sets provide a direct read-out of cellular phenotypes and are increasingly generated to study biological questions. Our previous work revealed the potential of analyzing extracellular metabolomic data in the context of the metabolic ...
Aurich, Maike K., Fleming, Ronan M. T., Thiele, Ines   +2 more
core   +3 more sources

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis

Biotechnology for Biofuels, 2017
BackgroundSynechococcus elongatus UTEX 2973 is the fastest growing cyanobacterium characterized to date. Its genome was found to be 99.8% identical to S. elongatus 7942 yet it grows twice as fast.
Mary H. Abernathy, Jingjie Yu, Fangfang Ma, M. Liberton, Justin L. Ungerer, Whitney D. Hollinshead, Saratram Gopalakrishnan, Lian He, C. Maranas, H. Pakrasi, Doug K. Allen, Yinjie J. Tang   +11 more
semanticscholar   +1 more source

Elementary vectors and conformal sums in polyhedral geometry and their relevance for metabolic pathway analysis [PDF]

, 2016
A fundamental result in metabolic pathway analysis states that every flux mode can be decomposed into a sum of elementary modes. However, only a decomposition without cancelations is biochemically meaningful, since a reversible reaction cannot have ...
Müller, Stefan, Regensburger, Georg
core   +3 more sources

Metabolic flux phenotyping of secondary metabolism in cyanobacteria

Trends in Microbiology, 2023
Cyanobacteria generate energy from photosynthesis and produce various secondary metabolites with diverse commercial and pharmaceutical applications. Unique metabolic and regulatory pathways in cyanobacteria present new challenges for researchers to enhance their product yields, titers, and rates. Therefore, further advancements are critically needed to
Piyoosh K. Babele, Amit Srivastava, Jamey D. Young   +2 more
openaire   +3 more sources