Results 81 to 90 of about 2,439,575 (338)

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Flux imbalance analysis and the sensitivity of cellular growth to changes in metabolite pools [PDF]

, 2013
Stoichiometric models of metabolism, such as flux balance analysis (FBA), are classically applied to predicting steady state rates - or fluxes - of metabolic reactions in genome-scale metabolic networks. Here we revisit the central assumption of FBA, i.e.
Mehta, Pankaj, Reznik, Ed, Segrè, Daniel   +2 more
core  

Low Degree Metabolites Explain Essential Reactions and Enhance Modularity in Biological Networks [PDF]

, 2005
Recently there has been a lot of interest in identifying modules at the level of genetic and metabolic networks of organisms, as well as in identifying single genes and reactions that are essential for the organism.
Giri, Varun, Jain, Sanjay, Krishna, Sandeep, Raghuram, N., Samal, Areejit, Singh, Shalini   +5 more
core   +4 more sources

The role of histone modifications in transcription regulation upon DNA damage

FEBS Letters, EarlyView.
This review discusses the critical role of histone modifications in regulating gene expression during the DNA damage response (DDR). By modulating chromatin structure and recruiting repair factors, these post‐translational modifications fine‐tune transcriptional programmes to maintain genomic stability.
Angelina Job Kolady, Siyao Wang
wiley   +1 more source

Abstracts of the 2023 Autumn Meeting of the British Society for Cardiovascular Research

Medical Sciences Forum
The Autumn Meeting of the British Society for Cardiovascular Research in 2023 was organized by Carolyn Carr, Lisa Heather and Claudia Montes Aparicio at Wadham College at the University of Oxford and was the 50th Anniversary Meeting of the Society.
Carolyn Carr
doaj   +1 more source

Systematic Applications of Metabolomics in Metabolic Engineering

Metabolites, 2012
The goals of metabolic engineering are well-served by the biological information provided by metabolomics: information on how the cell is currently using its biochemical resources is perhaps one of the best ways to inform strategies to engineer a cell to
Robert A. Dromms, Mark P. Styczynski
doaj   +1 more source

Metabolic fluxes for nutritional flexibility of Mycobacterium tuberculosis

Molecular Systems Biology, 2021
The co‐catabolism of multiple host‐derived carbon substrates is required by Mycobacterium tuberculosis (Mtb) to successfully sustain a tuberculosis infection. However, the metabolic plasticity of this pathogen and the complexity of the metabolic networks
Khushboo Borah, Tom A Mendum, Nathaniel D Hawkins, Jane L Ward, Michael H Beale, Gerald Larrouy‐Maumus, Apoorva Bhatt, Martine Moulin, Michael Haertlein, Gernot Strohmeier, Harald Pichler, V Trevor Forsyth, Stephan Noack, Celia W Goulding, Johnjoe McFadden, Dany J V Beste   +15 more
doaj   +1 more source

Quantitative constraint-based computational model of tumor-to-stroma coupling via lactate shuttle [PDF]

, 2015
Cancer cells utilize large amounts of ATP to sustain growth, relying primarily on non-oxidative, fermentative pathways for its production.
CAPUANI, FABRIZIO, DE MARTINO, ANDREA, DE MARTINO, DANIELE, MARINARI, Vincenzo   +3 more
core   +1 more source

Flux-Balance Modeling of Plant Metabolism [PDF]

Frontiers in Plant Science, 2011
Flux-balance modeling of plant metabolic networks provides an important complement to (13)C-based metabolic flux analysis. Flux-balance modeling is a constraints-based approach in which steady-state fluxes in a metabolic network are predicted by using optimization algorithms within an experimentally bounded solution space.
Sweetlove, L, Ratcliffe, R
openaire   +3 more sources

Mechanisms of parasite‐mediated disruption of brain vessels

FEBS Letters, EarlyView.
Parasites can affect the blood vessels of the brain, often causing serious neurological problems. This review explains how different parasites interact with and disrupt these vessels, what this means for brain health, and why these processes matter. Understanding these mechanisms may help us develop better ways to prevent or treat brain infections in ...
Leonor Loira, Sílvia Arroz‐Madeira, Cláudio A. Franco, Sara Silva Pereira   +3 more
wiley   +1 more source