Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Comparing different metadata application profiles for agricultural learning repositories [PDF]
, 2008 Agricultural learning repositories can provide new opportunities for sharing, accessing, using and reusing learning resources online. Metadata plays a crucial role in such systems: apart from simply indexing resources, metadata makes it easier to ...
Salokhe, Gauri +2 more
core +1 more source
zetoc: a Dublin Core based current awareness service [PDF]
, 2001 zetoc is a current awareness service for UK Higher and Further Education providing Z39.50 access to the British Library's Electronic Table of Contents database of journal articles and conference papers.
MacIntyre, Ross +3 more
core
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Building Quality Assurance into Metadata Creation: an Analysis based on the Learning Objects and e-Prints Communities of Practice [PDF]
, 2003 This paper challenges some of the assumptions underlying the metadata creation process in the context of two communities of practice, based around learning object repositories and open e-Print archives.
Hey, Jessie M. N. +6 more
core +2 more sources
Compatibility of University Websites with Metadata Standards: A Case Study on Shahed University
Iranian Journal of Information Processing & Management, 2016 The present study has been conducted to study the Shahed University's website in terms of compatibility with metadata standards (Dublin core, Mark XML. and descriptive object-oriented) and HTML meta tags.
Saeid Asadi +2 more
doaj
Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA
FEBS Open Bio, EarlyView.Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley +1 more source
The Benefits and Future of Standards: Metadata and Beyond [PDF]
, 2010 This article discusses the benefits and future of standards and presents the generic multi-dimensional Reference Model. First the importance and the tasks of interoperability as well as quality development and their relationship are analyzed. Especially in e-Learning their connection and interdependence is evident: Interoperability is one basic ...
openaire +3 more sources
Deep Learning Pose Estimation for Phenotyping of Co‐Occurring Hyperkinetic Movement Disorders
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective To explore whether routine outpatient video combined with deep learning‐based pose estimation and clinically interpretable kinematic features can support multi‐label phenotyping of co‐occurring hyperkinetic movement disorders (HMDs).
Laura Cif +17 more
wiley +1 more source
Towards Standardization: A Participatory Framework for Scientific Standard-Making
International Journal of Digital Curation, 2013 In contemporary scientific research, standard-making and standardization are key processes for the sharing and reuse of data. The goals of this paper are twofold: 1) to stress that collaboration is crucial to standard-making, and 2) to urge recognition ...
Lynn Yarmey, Karen S. Baker
doaj +5 more sources