Results 41 to 50 of about 252,611 (268)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Multicolor Histochemical Staining for Identification of Mineralized and Non-Mineralized Musculoskeletal Tissue: Immunohistochemical and Radiological Validation in Decalcified Bone Samples

Bioengineering, 2022
Histochemical staining of paraffin-embedded decalcified bone samples is commonly used in preclinical research of musculoskeletal diseases, enabling the visualization of multiple tissue components by the application of chromogens.
Yu Sun, Heike Helmholz, Regine Willumeit-Römer   +2 more
doaj   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Desarrollo de efectos cerámicos como acabados superficiales, mediante tecnología de inyección digital

Boletín de la Sociedad Española de Cerámica y Vidrio, 2012
Ferro Spain SA has tackled the practical viability of tile surfaces decoration by means of applying layers of reduced thickness by means of the use of digital injection technology by inkjet and, specifically, relating to effects and superficial finishes ...
Spain S.A., Ferro
doaj  

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

İMKB’ye Kayıtlı Seçilmiş İşletmelere Yönelik Etkinlik Ölçümü ve Performans Değerlendirmesi: Veri Zarflama Analizi ve Topsis Uygulaması

Selçuk Üniversitesi Sosyal Bilimler Enstitüsü Dergisi, 2012
Nüfusun hızla artmakta olduğu dünyada bireylerin ihtiyaçlarınıkarşılamak ve gelişmekte olan teknolojiler karşısında esnek ve uygun bir yapıya sahip olmak işletmeler için büyük bir önem arz etmektedir.
Mustafa Soba, Fatma Akcanlı, Işıl Erem   +2 more
doaj  

Assessing the Safety and Efficacy of Self-Expanding Metallic Y Stents in a Community Medicine Setting

Journal of Respiration
Self-Expanding Metallic (SEM) Y stents are a newer form of stent that is gaining popularity outside the USA, but still has limited data, especially in a community medicine setting.
Daniel Jacob Smith, Russell Vo, Parker Lachowsky, Ann Davis, Isha Puri, Sai Karan Vamsi Guda   +5 more
doaj   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Entropy of Conduction Electrons from Transport Experiments

Entropy, 2020
The entropy of conduction electrons was evaluated utilizing the thermodynamic definition of the Seebeck coefficient as a tool. This analysis was applied to two different kinds of scientific questions that can—if at all—be only partially ...
Nicolás Pérez, Constantin Wolf, Alexander Kunzmann, Jens Freudenberger, Maria Krautz, Bruno Weise, Kornelius Nielsch, Gabi Schierning   +7 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source