Comparative proteomic analysis of mature and immature oocytes in domestic cats
Asian Pacific Journal of Reproduction, 2020 Objective: To evaluate changes of feline (Felis catus) oocytes proteins during in vitro maturation by using the proteomic approach. Methods: Immature oocytes (germinal vesicle) isolated from female cats were cultured and collected at 0 h and 24 h.
Bongkoch Turathum +6 more
doaj +1 more source
Sorting nexin 9 recruits clathrin heavy chain to the mitotic spindle for chromosome alignment and segregation. [PDF]
PLoS ONE, 2013 Sorting nexin 9 (SNX9) and clathrin heavy chain (CHC) each have roles in mitosis during metaphase. Since the two proteins directly interact for their other cellular function in endocytosis we investigated whether they also interact for metaphase and ...
Maggie P C Ma +2 more
doaj +1 more source
The Small but Versatile Cyclin-Dependent Kinase Subunit CKS: C. elegans CKS-1 Regulates Anaphase Onset and Is Required for Anaphase B During Oocyte Meiosis. [PDF]
BioessaysThe cyclin‐dependent kinase subunit, CKS, is required for anaphase onset during both meiosis I and II in Caenorhabditis elegans oocytes. One model for how CKS promotes meiosis II anaphase onset involves phosphorylation of a Cullin 2 E3 ligase by CDK/CKS/CYB‐3, followed by the CKS anion pocket binding to the phosphorylated E3, recruiting CDK/CKS/CYB‐1 ...
Yang J, Bowerman B.
europepmc +2 more sources
Metaphase and Interphase Cytogenetics with Alu-PCR-amplified Yeast Artificial Chromosome Clones Containing the BCR Gene and the Protooncogenes c-raf-1, c-fms, and c-erbB-21 [PDF]
, 1992 A human yeast artificial chromosome (YAC) library was screened by polymerase chain reaction with oligonucleotide primers defined for DNA sequences of the BCR gene and the protooncogenes c-raf-1, c-fms, and c-erB-2.
Olson, Maynard V. +17 more
core +1 more source
DNA damage response and spindle assembly checkpoint function throughout the cell cycle to ensure genomic integrity. [PDF]
PLoS Genetics, 2015 Errors in replication or segregation lead to DNA damage, mutations, and aneuploidies. Consequently, cells monitor these events and delay progression through the cell cycle so repair precedes division.
Katherine S Lawrence +2 more
doaj +1 more source
Российский паразитологический журнал, 2016 Objective of research: to study the caryopathic effect of bio-preparation Bacillus subtilis 12В on the status of spermatogenic epithelium of white mice after a single intra-abdominal administration of the Fasciola hepatica extract. Materials and methods:
E. V. Krasnikova +2 more
doaj +2 more sources
Journal of Animal Reproduction and Biotechnology, 2020 Karyotype analysis is a major work in the process of triploid abalone production for the purpose of productivity and quality improvement. However, the metaphase spreads for karyotype analysis have been prepared just from the larvae at trochophore stage ...
Jae Hoon Choi +4 more
doaj +1 more source
Alternative Agents to Colcemid for Obtaining High-Quality Metaphase Spreads
AnimalsIn cytogenetics, the ability to perform FISH (Fluorescence In Situ Hybridization) experiments using probes that map very closely together depends on the capacity to produce sufficiently long chromosomes. Traditionally, colcemid is the chemical agent used
Michele Zannotti +2 more
doaj +1 more source
The equatorial position of the metaphase plate ensures symmetric cell divisions
eLife, 2015 Chromosome alignment in the middle of the bipolar spindle is a hallmark of metazoan cell divisions. When we offset the metaphase plate position by creating an asymmetric centriole distribution on each pole, we find that metaphase plates relocate to the ...
Chia Huei Tan +6 more
doaj +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source