Results 101 to 110 of about 8,720 (209)

High-throughput genetics enables identification of nutrient utilization and accessory energy metabolism genes in a model methanogen

mBio
Archaea are widespread in the environment and play fundamental roles in diverse ecosystems; however, characterization of their unique biology requires advanced tools. This is particularly challenging when characterizing gene function.
Leslie A. Day, Hans K. Carlson, Dallas R. Fonseca, Adam P. Arkin, Morgan N. Price, Adam M. Deutschbauer, Kyle C. Costa   +6 more
doaj   +1 more source

Diversity of prokaryotes at a shallow submarine vent of Panarea Island (Italy) by high-throughput sequencing

Atti della Accademia Peloritana dei Pericolanti : Classe di Scienze Fisiche, Matematiche e Naturali, 2013
To determine microbial community composition and possible key microbial processes in the shallow-sea hydrothermal vent system off Panarea Island (Italy), we examined bacterial and archaeal communities of sediment and fluid samples from a hot vent by 16S ...
Teresa L. Maugeri, Concetta Gugliandolo, Valeria Lentini   +2 more
doaj   +1 more source

Characterization of the Methanococcus jannaschii RNase P holoenzyme.

, 2000
The RNase P RNAs from Methanococcus and Archaeoglobus lack secondary structural features essential for the recognition of pre-tRNA. In the absence of protein, these RNase P RNAs lack catalytic activity.
Andrews, Andrew J.
core  

RB-TnSeq data for Methanococcus maripaludis S2 and Methanococcus maripaludis JJ

This data set comprises high-throughput genetic data for two strains of Methanococcus maripaludis, strain S2 and strain JJ, from RB-TnSeq (randomly barcoded transposon site sequencing).
Leslie Day (17905479), Morgan Price (3397133)   +1 more
core   +1 more source

Characterization of guanine and hypoxanthine phosphoribosyltransferases in Methanococcus voltae

Journal of Bacteriology, 1996
Phosphoribosyltransferase (PRTase) and nucleoside phosphorylase (NPase) activities were detected by radiometric methods in extracts of Methanococcus voltae. Guanine PRTase activity was present at 2.7 nmol min(-1) mg of protein(-1) and had an apparent Km for guanine of 0.2 mM and a pH optimum of 9. The activity was inhibited 50% by 0.3 mM GMP.
T L, Bowen, W C, Lin, W B, Whitman
openaire   +3 more sources

Methanococcus frisius sp.nov., a new methylotrophic marine methanogen

, 1986
A Gram-negative, nonmotile, coccoid methanogenic bacterium of 0.9–1.6 μm in diameter was isolated from shoal mud of the southern North Sea (West Germany). The isolate grew on H2 + CO2, methanol, and methylamines.
Karl Heinz Lüpkes, Karl Heinz Blotevogel, Ulrich Fischer   +2 more
core   +1 more source

Rare Biosphere Archaea Assimilate Acetate in Precambrian Terrestrial Subsurface at 2.2 km Depth

Geosciences, 2018
The deep biosphere contains a large portion of the total microbial communities on Earth, but little is known about the carbon sources that support deep life.
Maija Nuppunen-Puputti, Lotta Purkamo, Riikka Kietäväinen, Mari Nyyssönen, Merja Itävaara, Lasse Ahonen, Ilmo Kukkonen, Malin Bomberg   +7 more
doaj   +1 more source

Heat shock response of the archaebacterium Methanococcus voltae

Journal of Bacteriology, 1991
The general properties of the heat shock response of the archaebacterium Methanococcus voltae were characterized. The induction of 11 heat shock proteins, with apparent molecular weights ranging from 18,000 to 90,000, occurred optimally at 40 to 50 degrees C. Some of the heat shock proteins were preferentially enriched in either the soluble (cytoplasm)
A M, Hebert, A M, Kropinski, K F, Jarrell   +2 more
openaire   +3 more sources

Effects of N-glycosylation site removal in archaellins on the assembly and function of archaella in Methanococcus maripaludis.

PLoS ONE, 2015
In Methanococcus maripaludis S2, the swimming organelle, the archaellum, is composed of three archaellins, FlaB1S2, FlaB2S2 and FlaB3S2. All three are modified with an N-linked tetrasaccharide at multiple sites.
Yan Ding, Kaoru Uchida, Shin-Ichi Aizawa, Kathleen Murphy, Alison Berezuk, Cezar M Khursigara, James P J Chong, Ken F Jarrell   +7 more
doaj   +1 more source

Shuttle Vector System for Methanococcus maripaludis with Improved Transformation Efficiency

, 2011
We have identified an open reading frame and DNA element that are sufficient to maintain shuttle vectors in Methanococcus maripaludis . Strain S0001, containing ORF1 from pURB500 integrated into the M. maripaludis
Sarah E. Smith, James P. J. Chong, Alison D. Walters   +2 more
core   +1 more source