Results 251 to 260 of about 179,944 (271)

Methanol Dehydrogenase from Nocardia SP. 239

1989
Gram-negative, methylotrophic bacteria convert methanol into formaldehyde via NAD(P)-independent methanol dehydrogenase (MDH) with pyrroloquinoline quinone (PQQ) as the cofactor. The activity of MDH can be measured in vitro at pH 9 in the presence of ammonium salts as activator and with artificial dyes as electron acceptor (Anthony, 1982).
P. W. van Ophem, J. A. Duine
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X-ray structure of PQQ-dependent methanol dehydrogenase

1994
The three-dimensional structure of the PQQ-dependent quinoprotein, methanol dehydrogenase from Methylobacterium extorquens AM1, has been determined at 3A resolution. The a2b2 tetrameric enzyme has a large a-chain of almost spherical form with a chain fold in which eight 4-stranded antiparallel b-sheets segments are arranged radially around a pseudo 8 ...
M, Ghosh, A, Avezoux, C, Anthony, K, Harlos, C C, Blake   +4 more
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Methanol dehydrogenase bioelectrochemical cell and alcohol detector

Biotechnology Letters, 1981
A bioelectrochemical cell is described in which bacterial methanol dehydrogenase is used to effect the efficient transduction of chemical into electrical energy. The cell can also be used as a sensitive detector for a variety of primary alcohols in the range, 10-7 – 5×10-11 moles.
E.V. Plotkin, I.J. Higgins, H.A.O. Hill
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In Silico Studies of the Mechanism of Methanol Oxidation by Quinoprotein Methanol Dehydrogenase

Journal of the American Chemical Society, 2003
The mechanism of bacterial methanol dehydrogenase involves hydride equivalent transfer from substrate to the ortho-quinone PQQ to provide a C5-reduced intermediate that subsequently rearranges to the hydroquinone PQQH(2). We have studied the PQQ reduction by molecular dynamic (MD) simulations in aqueous solution.
Swarnalatha Y, Reddy, Thomas C, Bruice
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Electrochemical conversion of carbon dioxide to methanol with the assistance of formate dehydrogenase and methanol dehydrogenase as biocatalysts

Journal of the American Chemical Society, 1994
Electrolysis at potentials between -0.7 and -0.9 V vs SCE of carbon dioxide-saturated phosphate buffer solutions (pH7) containing formate dehydrogenase (FDH) and either methyl viologen (MV[sup 2+]) or pyrroloquinolinequinone (PQQ) as an electron mediator yielded formate with current efficiencies as high as 90%.
Susumu Kuwabata, Ryo Tsuda, Hiroshi Yoneyama   +2 more
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Purification and properties of methanol dehydrogenase from Methylophaga marina

Biochimie, 1987
Purification of methanol dehydrogenase from Methylophaga marina, in order to avoid the instability observed in crude extracts, was achieved initially by a rapid procedure using mainly an aqueous two-phase partition system composed of polyethylene glycol 1000 (50%, v/v) and potassium phosphate (50%, w/v).
M, Janvier, F, Gasser
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Purification and properties of methanol dehydrogenase from Hyphomicrobium X

Biochimica et Biophysica Acta (BBA) - Enzymology, 1978
(1) A method for the isolation of methanol dehydrogenase (alcohol:(acceptor) oxidoreductase, EC 1.1.99.8) from Hyphomicrobium X is decribed. The purified enzyme was resolved by polyacrylamide gel electrophoresis into one main and two minor active bands. Iron and manganese were the only detected metals in the enzyme preparation.
J A, Duine, J, Frank, J, Westerling
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Novel methanol dehydrogenase enzymes from bacillus

2013
The present invention relates to a nucleic acid molecule, which encodes a polypeptide having alcohol dehydrogenase activity, in particular methanol dehydrogenase activity, comprising having a nucleotide sequence selected from the group consisting of: (i) a nucleotide sequence as set forth in any one of SEQ ID NOs: 1 (mdh2-MGA3), 3 (mdh3-MGA3), or 5 ...
Brautaset, Trygve, HEGGESET, Tonje Marita Bjerkan, KROG, Anne, Quax, Wilhelmus Johannes, SIBBALD, Mark Jan Jacobus Bernhard, VORHOLT, Julia, Müller, Jonas, Kiefer, Patrick, POTTHOFF, Eva, Wendisch, Volker F., LESSMEIER, Lennart, HEUX, Stéphanie, PORTAIS, Jean-Charles   +12 more
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PQQ-Dependent Methanol Dehydrogenase from Clostridium Thermoautotrophicum

1989
The incorporation of methanol into acetate by acetogenic bacteria has been investigated. A PQQ-dependent methanol dehydrogenase, that oxidizes methanol and formaldehyde to formate, has been purified from C. thermoautotrophicum.
Debra K. Winters, Lars G. Ljungdahl
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Formate dehydrogenase activity in methanol-utilizing yeasts.

Microbios, 1994
The growth and activity of formate dehydrogenase (FDH) in five methanol-utilizing yeasts at various methanol concentrations were investigated. The parameters observed were inhibited at 4% methanol concentration in the medium. For Candida boidinii and Pichia trehalophila FDH activity was not found.
V, Illeová, M, Certík, V, Stefuca, V, Báles   +3 more
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