Results 71 to 80 of about 4,218 (198)

Genomic and Transcriptomic Insights Into the Autotrophic Metabolism on H2 + CO2 or CO of the Thermophilic Acetogenic Model Organism Moorella thermoacetica

Environmental Microbiology, Volume 28, Issue 4, April 2026.
Moorella thermoacetica is the model organism for elucidation of acetogenesis. The reduction of CO2 to acetate is well studied, but how redox carrier balancing and energy conservation are maintained during autotrophic growth and how H2 is oxidised remains enigmatic.
Florian P. Rosenbaum, Anja Poehlein, Rolf Daniel, Volker Müller   +3 more
wiley   +1 more source

Three-dimensional Organization of the Archaeal A1-ATPase from Methanosarcina mazei Gö1 [PDF]

Journal of Biological Chemistry, 2004
A modified isolation procedure provides a homogeneous A(1)-ATPase from the archaeon Methanosarcina mazei Gö1, containing the five subunits in stoichiometric amounts of A(3):B(3):C:D:F. A(1) obtained in this way was characterized by three-dimensional electron microscopy of single particles, resulting in the first three-dimensional reconstruction of an A(
Unal, Coskun, Michael, Radermacher, Volker, Müller, Teresa, Ruiz, Gerhard, Grüber   +4 more
openaire   +2 more sources

Metabolic flexibility as a major predictor of spatial distribution in microbial communities [PDF]

, 2014
A better understand the ecology of microbes and their role in the global ecosystem could be achieved if traditional ecological theories can be applied to microbes. In ecology organisms are defined as specialists or generalists according to the breadth of
A Colles, AM Flynn, BB Jørgensen, BB Oakley, BB Oakley, BB Oakley, Brian B. Oakley, CA Hanson, CJ Robinson, CL Lauber, DB Nedwell, Douglas Andrew Campbell, EK Costello, F Carbonero, FM Cohan, Franck Carbonero, GM Spooner, HJ Laanbroek, JI Prosser, Kevin J. Purdy, KJ Gaston, KJ Gaston, KJ Purdy, KJ Purdy, KJ Purdy, KJ Purdy, KJ Purdy, L Philippot, MA Leibold, MF Fitzsimons, N Fierer, N Mantel, P Cermeno, PG Falkowski, R Julliard, R Kassen, RH MacArthur, RJ Hawkins, RJ Whitaker, RJ Whittaker, T Poisot, TP Curtis, V Devictor   +42 more
core   +3 more sources

Evolutionary divergence and functional insights into the heteromeric cis‐prenyltransferase of Paramecium tetraurelia

The FEBS Journal, Volume 293, Issue 6, Page 1681-1707, March 2026.
Heteromeric cis‐prenyltransferases (CPT) are indispensable for dolichol synthesis and protein N‐glycosylation in most eukaryotes. The catalytic subunits are strongly conserved throughout evolution, in contrast to the evolutionarily variable accessory subunits. The POC1 protein from Paramecium tetraurelia is the smallest identified CPT‐accessory subunit
Agnieszka Onysk, Kamil Steczkiewicz, Mariusz Radkiewicz, Paweł Link‐Lenczowski, Przemysław Surowiecki, Karolina Sztompka, Kariona A. Grabińska, Jacek K. Nowak, Liliana Surmacz   +8 more
wiley   +1 more source

Structural conservation of chemotaxis machinery across Archaea and Bacteria [PDF]

, 2015
Chemotaxis allows cells to sense and respond to their environment. In Bacteria, stimuli are detected by arrays of chemoreceptors that relay the signal to a two-component regulatory system.
Briegel, Ariane, Gunsalus, Robert P., Huang, Audrey N., Jensen, Grant J., Oikonomou, Catherine M., Ortega, Davi R.   +5 more
core   +3 more sources

Genetic Encoding of a Trifunctional Photo‐Cross‐Linker with a Cleavable Alkyl Ester Moiety

ChemBioChem, Volume 27, Issue 3, 12 February 2026.
A novel noncanonical amino acid, DiZAAsu, possessing photo‐cross‐linking, biotinylation, and alkaline‐cleavable functionalities, is genetically encoded by an engineered pyrrolyl‐tRNA synthetase mutant. The alkyl ester moiety expands the design space of cleavable photo‐cross‐linkable amino acids.
Masahiro Takayama, Tomoya Tsubota, Takao Yamaguchi, Kosuke Chiba, Takumi Yoshida, Yoshiyuki Hari, Yu‐Shi Tian, Daisuke Takaya, Asuka Mori, Tomohito Tsukamoto, Kenji Ishimoto, Yukio Ago, Yoshiaki Okada, Kensaku Sakamoto, Takefumi Doi, Kaori Fukuzawa, Satoshi Obika, Shinsaku Nakagawa, Nobumasa Hino   +18 more
wiley   +1 more source

2-oxoglutarate triggers assembly of active dodecameric Methanosarcina mazei glutamine synthetase

eLife
Abstract Glutamine synthetases (GS) are central enzymes essential for the nitrogen metabolism across all domains of life. Consequently, they have been extensively studied for more than half a century. Based on the ATP dependent ammonium assimilation generating glutamine, GS expression and activity are strictly ...
Herdering E, Reif-Trauttmansdorff T, Kumar A, Habenicht T, Hochberg G, Bohn S, Schuller J, Schmitz RA.   +7 more
europepmc   +6 more sources

In Vivo Biosynthesis and Direct Incorporation of Noncanonical Amino Acids into Proteins

ChemBioChem, Volume 26, Issue 22, November 17, 2025.
This review evaluates engineered (semi)autonomous cell systems for the biosynthesis and incorporation of noncanonical amino acids (ncAAs) into proteins. While semi‐autonomous cells convert supplied precursors into ncAAs autonomous cells integrate biosynthetic pathways that produce these building blocks intracellularly.
Jan Hendrik Illies, Tim Moritz Weber, Ivana Drienovská   +2 more
wiley   +1 more source

Changes in concentrations of coenzyme F420 analogs during batch growth of Methanosarcina barkeri and Methanosarcina mazei [PDF]

Applied and Environmental Microbiology, 1989
Coenzyme F420 has been assayed by high-performance liquid chromatography with fluorimetric detection; this permits quantification of individual coenzyme F420 analogs whilst avoiding the inclusion of interfering material. The total intracellular coenzyme F420 content of Methanosarcina barkeri MS cultivated on methanol and on H2-CO2 and of Methanosarcina
openaire   +2 more sources

Structural insight into substrate and product binding in an archaeal mevalonate kinase.

PLoS ONE, 2018
Mevalonate kinase (MK) is a key enzyme of the mevalonate pathway, which produces the biosynthetic precursors for steroids, including cholesterol, and isoprenoids, the largest class of natural products.
Bradley R Miller, Yan Kung
doaj   +1 more source