Results 61 to 70 of about 5,691 (138)

In Vivo Biosynthesis and Direct Incorporation of Noncanonical Amino Acids into Proteins

ChemBioChem, Volume 26, Issue 22, November 17, 2025.
This review evaluates engineered (semi)autonomous cell systems for the biosynthesis and incorporation of noncanonical amino acids (ncAAs) into proteins. While semi‐autonomous cells convert supplied precursors into ncAAs autonomous cells integrate biosynthetic pathways that produce these building blocks intracellularly.
Jan Hendrik Illies, Tim Moritz Weber, Ivana Drienovská   +2 more
wiley   +1 more source

Functional organization of a single nif cluster in the mesophilic archaeon Methanosarcina mazei strain Gö1

, 2002
The mesophilic methanogenic archaeon Methanosarcina mazei strain Gö1 is able to utilize molecular nitrogen (N2) as its sole nitrogen source. We have identified and characterized a single nitrogen fixation (nif) gene cluster in M.
Veit, Katharina, Ehlers, Claudia, Gottschalk, Gerhard, Schmitz, Ruth A.   +3 more
core   +1 more source

Microbial Conversion of Glycerol Into 1,3‐Propanediol by Fermentation: Review of Fundamentals and Operational Strategies

Microbial Biotechnology, Volume 18, Issue 11, November 2025.
This review highlights recent advances in microbial fermentation strategies for converting glycerol, a biodiesel by‐product, into 1,3‐propanediol. It discusses metabolic pathways, microbial inocula and key operational parameters to optimise production, supporting sustainable chemical synthesis aligned with circular economy principles. ABSTRACT Over the
María Fernanda Pérez‐Bernal, Roman Moscoviz, Xiaoli Wang, Nicolas Bernet, Eric Trably   +4 more
wiley   +1 more source

Conversion of Serine-Type Aldehyde Tags by the Radical SAM Protein AtsB from Methanosarcina mazei

, 2019
Krüger T, Weiland S, Boschanski M, et al. Conversion of Serine-Type Aldehyde Tags by the Radical SAM Protein AtsB from Methanosarcina mazei. ChemBioChem. 2019;20(16: Special Issue: 10th IPS):2074-2078.Formylglycine-generating enzymes provide a convenient
Müller, Kristian ; https://orcid.org/, Krüger, Tobias, Dierks, Thomas, Weiland, Stefanie, Boschanski, Mareile, Sinha, Prem Kumar, Falck, Georg, Sewald, Norbert ; https://orcid.org/   +7 more
core   +1 more source

Rational design of a covalent ACE2 decoy receptor that broadly neutralizes SARS‐CoV‐2 variants

Protein Science, Volume 34, Issue 10, October 2025.
Abstract The ongoing evolution of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) and the emergence of variants that evade existing vaccines and antibody therapies necessitate novel, potent, and broad‐spectrum antiviral strategies. Enhancing therapeutic proteins with additional covalent binding capabilities, such as minibinders and ...
Nobumasa Hino, Risa Takada, Kyosuke Suzuki, Nagisa Tokunoh, Tatsuya Karaki, Kazumasa Ohtake, Haruna Ogami, Takafumi Nishiura, Kenji Ishimoto, Tomohito Tsukamoto, Yukio Ago, Yoshiaki Okada, Toru Okamoto, Chikako Ono, Yoshiharu Matsuura, Satoshi Obika, Kensaku Sakamoto, Yasuo Yoshioka, Shinsaku Nakagawa   +18 more
wiley   +1 more source

COMPLETE GENOME SEQUENCE OF THE TYPE STRAIN BACTERIUM SPHAEROCHAETA ASSOCIATA GLS2T (VKM B-2742)T

Journal of Bioinformatics and Genomics
This study reports the complete genome sequence of Sphaerochaeta associata GLS2T (=VKM B-2742T =DSM 26261T), which was isolated from a consortium with methanogenic archaeon Methanosarcina mazei JL01.
Troshina O.Y., Tepeeva A.N., Arzamastseva V.O., Whitman W.B., Varghese N.J., Shapiro N., Woyke T., Kyrpides N.C., Vasilenko O.V.   +8 more
doaj   +1 more source

Qualitative Analysis of Microbial Dynamics during Anaerobic Digestion of Microalgal Biomass in a UASB Reactor

International Journal of Microbiology, 2017
Anaerobic digestion (AD) is a microbiologically coordinated process with dynamic relationships between bacterial players. Current understanding of dynamic changes in the bacterial composition during the AD process is incomplete.
Anna Doloman, Yousef Soboh, Andrew J. Walters, Ronald C. Sims, Charles D. Miller   +4 more
doaj   +1 more source

Expanding the Scope of Orthogonal Translation with Pyrrolysyl-tRNA Synthetases Dedicated to Aromatic Amino Acids

Molecules, 2020
In protein engineering and synthetic biology, Methanosarcina mazei pyrrolysyl-tRNA synthetase (MmPylRS), with its cognate tRNAPyl, is one of the most popular tools for site-specific incorporation of non-canonical amino acids (ncAAs).
Hsueh-Wei Tseng, Tobias Baumann, Huan Sun, Yane-Shih Wang, Zoya Ignatova, Nediljko Budisa   +5 more
doaj   +1 more source

An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids

Bioresources and Bioprocessing, 2023
Aminoacyl-tRNA synthetase (aaRS) is a core component for genetic code expansion (GCE), a powerful technique that enables the incorporation of noncanonical amino acids (ncAAs) into a protein. The aaRS with polyspecificity can be exploited in incorporating
Ke Liu, Ling Jiang, Shuang Ma, Zhongdi Song, Lun Wang, Qunfeng Zhang, Renhao Xu, Lirong Yang, Jianping Wu, Haoran Yu   +9 more
doaj   +1 more source

Carbon-dependent control of electron transfer and central carbon pathway genes for methane biosynthesis in the Archaean, Methanosarcina acetivorans strain C2A

BMC Microbiology, 2010
Background The archaeon, Methanosarcina acetivorans strain C2A forms methane, a potent greenhouse gas, from a variety of one-carbon substrates and acetate. Whereas the biochemical pathways leading to methane formation are well understood, little is known
Gunsalus Robert P, Rohlin Lars
doaj   +1 more source