Results 41 to 50 of about 5,691 (138)

Label-Free Raman Microspectroscopy for Identifying Prokaryotic Virocells

mSystems, 2022
Raman microspectroscopy has been used to thoroughly assess growth dynamics and heterogeneity of prokaryotic cells, yet little is known about how the chemistry of individual cells changes during infection with virulent viruses, resulting in so-called ...
Indra Monsees, Victoria Turzynski, Sarah P. Esser, André Soares, Lara I. Timmermann, Katrin Weidenbach, Jarno Banas, Michael Kloster, Bánk Beszteri, Ruth A. Schmitz, Alexander J. Probst   +10 more
doaj   +1 more source

Genetic Code Expansion System for Tight Control of Gene Expression in Bombyx mori Cell Lines

Insects, 2021
Inducible gene expression systems are important tools for studying gene function and to control protein synthesis. With the completion of the detailed map of the silkworm (Bombyx mori) genome, the study of Bombyx mori has entered the post-genome era ...
Wei Lu, Ruolin Wang, Pan Wang, Sanyuan Ma, Qingyou Xia   +4 more
doaj   +1 more source

The ether lipids of Methanosarcina mazei and other Methanosarcina species, compared by fast atom bombardment mass spectrometry

, 1994
The total lipids of Methanosarcina mazei accounted for 4.0% of the cell dry mass, and 90% of these were polar lipids. Nearly all of the polar fraction consisted of diether (2,3-di-O-phytanyl-sn-glycerol) and 3-hydroxydiether analogs of ...
Patel, G., Sprott, G., Dicaire, C.
core   +1 more source

Genomewide transcriptional Analysis of Methanosarcina mazei Gö1

, 2003
Es wurden Computerprogramme im Rahmen dieser Arbeit programmiert, die es ermöglichten DNA-Microarray-Chips zu generieren, die das gesamte Transkriptom von Methanosarcina mazei Gö1 repräsentieren.Ferner wurde ...
Hovey, Raymond Leonard
core   +1 more source

2,6,10,15,19-Pentamethylicosenes in Methanolobus bombayensis, a marine methanogenic archaeon, and in Methanosarcina mazei [PDF]

, 1997
2,6,10,15,19-Pentamethylicosenes (PMEs) containing three to five double bonds have been found in the methanogenic archaea Methanosarcina mazei (DSM 3338), a strain isolated from sewage sludge, and in Methanolobus bombayensis (OCM 438), a non ...
Sinninghe Damsté, Jaap S., Stefan Schouten, Maarel, Marc J.E.C. van der,, Damste, JSS, Schouten, S., Robert Huber, Sinninghe Damsté, J.S., Huber, Robert,, Huber, R., Huber, R, Marc J.E.C. Van Der Maarel, Jaap S.Sinninghe Damste, Schouten, Stefan,, VanderMaarel, MJEC, Sinninghe Damsté, Jaap S.,, Maarel, M.J.E.C. van der   +15 more
core   +3 more sources

Ferritin Conjugates With Multiple Clickable Amino Acids Encoded by C-Terminal Engineered Pyrrolysyl-tRNA Synthetase

Frontiers in Chemistry, 2021
This study reports the application of expanding genetic codes in developing protein cage-based delivery systems. The evolved Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS)•tRNAPyl pairs derived from directed evolution are examined to probe their
Yi-Hui Wang, Mu-Lung Jian, Mu-Lung Jian, Pei-Jung Chen, Pei-Jung Chen, Jo-Chu Tsou, Jo-Chu Tsou, Le P. Truong, Yane-Shih Wang, Yane-Shih Wang   +9 more
doaj   +1 more source

Structural and mutational analysis of Methanosarcina mazei prenylated FMN synthase reveals the basis of its unique prenyl donor substrate specificity

The FEBS Journal, EarlyView.
Some prenylated flavin mononucleotide (prFMN) synthases exceptionally prefer dimethylallyl phosphate (DMAP) over dimethylallyl diphosphate (DMAPP), which is a common prenyl donor substrate for many prenyltransferases. Structural and mutagenic analyses of Methanosarcina mazei prFMN synthase, which prefers DMAP but can also accept DMAPP, elucidated its ...
Sou Fukuhara, Hideaki Unno, Soma Ishimine, Ryuhei Nagata, Tomokazu Ito, Hisashi Hemmi   +5 more
wiley   +1 more source

Substratumsetzung und Schutz vor Sauerstoffradikalen in Methanosarcina mazei [PDF]

, 2011
Methanosarcina (Ms.) mazei ist ein methanogenes Archaeon, das methylierte C1-Verbindungen als Substrate verwenden kann. Bei Wachstum von Ms.
Krätzer, Christian
core   +1 more source

NMR-spektroskopische Untersuchungen zu Protein-Ligand-Wechselwirkungen [PDF]

, 2003
In der vorliegenden Doktorarbeit wurden die folgenden fünf biochemischen Fragestellungen zu Enzymen und deren Wechselwirkungen mit ihren Substraten mit NMR­ spektroskopischen Methoden untersucht: (1) Die spontane Bildungsrate von N­Carboxymethanofuran ...
Bartoschek, Stefan
core  

A Genetically Encoded Homocysteine Precursor to Probe Protein Active Sites and to Addict Escherichia coli to a Noncanonical Amino Acid Directly Involved in Catalysis

Angewandte Chemie, Volume 137, Issue 36, September 1, 2025.
A new genetically encoded amino acid is chemically converted into homocysteine (Hcy) under mild conditions to probe and to activate protein active sites. Surprisingly, Hcy can replace cysteine or serine residues in several enzymes. Its potential for a widespread use in proteins is further underlined by rendering Escherichia coli dependent on Hcy ...
Clara Dunker, Henning D. Mootz
wiley   +2 more sources