Results 71 to 80 of about 5,691 (138)

Survival of microorganisms during two-year exposure in outer space near the ISS

Scientific Reports
Results of an experiment named "Test" on survival and variability of microorganisms in open space near the International Space Station are presented. It was found after two-years exposure, spore-forming bacteria of the species Bacillus subtilis, fungi of
Elena A. Deshevaya, Svetlana V. Fialkina, Elena V. Shubralova, Oleg S. Tsygankov, Natalia M. Khamidullina, Leonid M. Vasilyak, Vladimir Ya. Pecherkin, Viktoria A. Shcherbakova, Andrey M. Nosovsky, Оleg I. Orlov   +9 more
doaj   +1 more source

Linker and N-Terminal Domain Engineering of Pyrrolysyl-tRNA Synthetase for Substrate Range Shifting and Activity Enhancement

Frontiers in Bioengineering and Biotechnology, 2020
The Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS)⋅tRNAPyl pair can be used to incorporate non-canonical amino acids (ncAAs) into proteins at installed amber stop codons.
Han-Kai Jiang, Han-Kai Jiang, Han-Kai Jiang, Man-Nee Lee, Jo-Chu Tsou, Kuan-Wen Chang, Hsueh-Wei Tseng, Kuang-Po Chen, Yaw-Kuen Li, Yane-Shih Wang, Yane-Shih Wang, Yane-Shih Wang   +11 more
doaj   +1 more source

ModE Regulates Alternative Nitrogenase Expression in the Methanogen Methanosarcina acetivorans

Molecular Microbiology, Volume 124, Issue 2, Page 105-114, August 2025.
During nitrogen fixation, the methanogen Methanosarcina acetivorans produces the alternative vanadium‐nitrogenase and iron‐nitrogenase only when molybdenum is unavailable to support the activity of molybdenum‐nitrogenase. Our work demonstrates that the expression of the alternative nitrogenases in M.
Melissa Chanderban, Daniel J. Lessner
wiley   +1 more source

Simplified methodology for a modular and genetically expanded protein synthesis in cell-free systems

Synthetic and Systems Biotechnology, 2019
Genetic code expansion, which enables the site-specific incorporation of unnatural amino acids into proteins, has emerged as a new and powerful tool for protein engineering.
Yonatan Chemla, Eden Ozer, Michael Shaferman, Ben Zaad, Rambabu Dandela, Lital Alfonta   +5 more
doaj   +1 more source

Spatiotemporal Control Over Protein Release from Artificial Cells via a Light‐Activatable Protease

Advanced Biology, Volume 9, Issue 5, May 2025.
Stimulus‐responsive protein release is essential for intercellular communication. Mimicking this functionality in artificial cells is promising to study the working principles of cellular signaling. Herein, an engineered light‐activatable protease is implemented in a coacervate‐based artificial cell platform to establish user‐defined spatiotemporal ...
Arjan Hazegh Nikroo, Wiggert J. Altenburg, Thijs W. van Veldhuisen, Luc Brunsveld, Jan C. M. van Hest   +4 more
wiley   +1 more source

Coexistence of group I and group II chaperonins in the archaeon Methanosarcina mazei

, 2003
Two distantly related classes of cylindrical chaperonin complexes assist in the folding of newly synthesized and stress-denatured proteins in an ATP-dependent manner.
Pfeifer, G., Hayer-Hartl, M., Muller, V., Figueiredo, L., Deppenmeier, U., Gottschalk, G., Haas, B., Hartl, F. U., Hirtreiter, A., Müller, Volker, Klunker, D., Naylor, D. J.   +11 more
core   +1 more source

Nature AND nurture: enabling formate‐dependent growth in Methanosarcina acetivorans

The FEBS Journal, Volume 292, Issue 9, Page 2251-2271, May 2025.
We identified a cytosolic formate dehydrogenase (FdhAB) present in several Methanosarcinales, likely acquired by independent horizontal gene transfers. We engineered two strains of Methanosarcina acetivorans: (a) JB‐MF and (b) JB‐F2 by functionally expressing FdhAB.
Jichen Bao, Tejas Somvanshi, Yufang Tian, Maxime G. Laird, Pierre Simon Garcia, Christian Schöne, Michael Rother, Guillaume Borrel, Silvan Scheller   +8 more
wiley   +1 more source

Methanogenic Conversion of 3-S-Methylmercaptopropionate to 3-Mercaptopropionate [PDF]

, 1995
Anaerobic metabolism of dimethylsulfoniopropionate, an osmolyte of marine algae, in anoxic intertidal sediments involves either cleavage to dimethylsulfide or demethylation to 3-S-methylmercaptopropionate (MMPA) and subsequently to 3-mercaptopropionate ...
Jansen, Michael,, Hansen, Theo A.,, Hansen, Theo A., Maarel, Marc J.E.C. van der,, Jansen, Michael, Maarel, Marc J.E.C. van der   +5 more
core   +1 more source

How Small Proteins Adjust the Metabolism of Cyanobacteria Under Stress

BioEssays, Volume 47, Issue 3, March 2025.
Cyanobacteria are powered by oxygenic photosynthesis located in extensive internal membrane systems, the thylakoids. Using carboxysomes, they assimilate organic from inorganic carbon. The integration of photosynthesis, carbon fixation, assimilation of nitrogen, and other nutrients involves several small proteins.
Alexander Kraus, Wolfgang R. Hess
wiley   +1 more source

Selection and assembly of indigenous bacteria and methanogens from spent metalworking fluids and their potential as a starting culture in a fluidized bed reactor

Microbial Biotechnology, 2019
Summary Waste metalworking fluids (MWFs) are highly biocidal resulting in real difficulties in the, otherwise favoured, bioremediation of these high chemical oxygen deman (COD) wastes anaerobically in bioreactors.
Ioannis Vyrides, Damian W. Rivett, Kenneth D. Bruce, Andrew K. Lilley   +3 more
doaj   +1 more source