Results 221 to 230 of about 582,283 (312)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

The Sport Publication Observational Research Tool (SPORT): An Objective Tool to Score the Methodological Quality of Observational Clinical Sports Medicine Research. [PDF]

Orthop J Sports Med
Kuhn AW, Inclan PM, Haider AA, Christy MN, Dunn WR, Alberton L, Allen C, Anderson K, Andrews J, Azar F, Baer G, Banffy M, Bedi A, Brockmeier S, Brophy R, Bush-Joseph C, Carey J, Carter T, Cohen S, Farrow L, Flanigan D, Franklin C, Hame S, Ioannis T, Joseph S, Kenter K, Koh J, Krych A, LeClere L, Lee C, Levy B, McAllister D, Medvecky M, Morganti C, Musahl V, Nelson B, Noyes F, Owens B, Pace L, Paulos L, Redler L, Rodeo S, Safran M, Savoie F, Shelbourne D, Sherman S, Singer K, Smith M, Sonnery-Cottet B, Spiker A, Stuart M, Warren R, Wittstein J, Wolcott M, Wright R, Matava MJ.   +55 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Clinical practice guidelines for acute kidney injury: a systematic review of the methodological quality. [PDF]

Front Med (Lausanne)
Li R, Wu L, Wu X, Fu Q, Xu M, Zhai X, Chen J.   +6 more
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Top 50 Most-Cited Articles on Tension Band Wiring for Patellar Fractures: A Bibliometric Analysis and Methodological Quality Assessment. [PDF]

Cureus
Zainy A, Lee CH, Sadik H, Qiao G.
europepmc   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Exploring the methodological quality and risk of bias in 200 systematic reviews: A comparative study of ROBIS and AMSTAR-2 tools. [PDF]

Res Synth Methods
Lunny C, Jain N, Nazari T, Kosaner-Kließ M, Santos L, Goodman I, Osman AAM, Berrone S, Dadam MN, Brenna CTA, Hussein H, Dahdal G, Cespedes A D, Ferri N, Kanji S, Chi Y, Pieper D, Shea B, Parker A, Neupane D, Khan PA, Rangira D, Kolaski K, Ridley B, Berour A, Sun K, Hamidi Rad R, Ouyang Z, Reid EK, Pérez-Neri I, Barakat SO, Bargeri S, Gianola S, Castellini G, Whitelaw S, Stevens A, Kolekar SB, Wong K, Major P, Bagheri E, Tricco AC.   +40 more
europepmc   +1 more source

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

FEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell, Cassy Ross, Panagiota S. Filippou, Bruno Silvester Lopes, Dominic Gilchrist, Paul Dean, Kamar Ameen‐Ali, Tora K. Smulders‐Srinivasan, Ahmad A. Khundakar   +8 more
wiley   +1 more source