Results 11 to 20 of about 516,288 (261)

Endonuclease-based genotyping of the RBM as a method to track the emergence or evolution of SARS-CoV-2 variants

iScience, 2021
Summary: Since the beginning of the COVID-19 pandemics, variants have emerged. Some of them display increased transmissibility and/or resistance to immune response.
Eva Lopez, Margot Barthélémy, Cécile Baronti, Shirley Masse, Alessandra Falchi, Fabien Durbesson, Renaud Vincentelli, Xavier de Lamballerie, Rémi Charrel, Bruno Coutard   +9 more
doaj   +1 more source

The 3D Reconstruction of Pocillopora Colony Sheds Light on the Growth Pattern of This Reef-Building Coral

iScience, 2020
Summary: Coral reefs are formed by living polyps, and understanding the dynamic processes behind the reefs is crucial for marine ecosystem restoration.
Yixin Li, Tingyu Han, Kun Bi, Kun Liang, Junyuan Chen, Jing Lu, Chunpeng He, Zuhong Lu   +7 more
doaj   +1 more source

A dual SHOX2:GFP; MYH6:mCherry knockin hESC reporter line for derivation of human SAN-like cells

iScience, 2022
Summary: The sinoatrial node (SAN) is the primary pacemaker of the heart. The human SAN is poorly understood due to limited primary tissue access and limitations in robust in vitro derivation methods.
Zaniar Ghazizadeh, Jiajun Zhu, Faranak Fattahi, Alice Tang, Xiaolu Sun, Sadaf Amin, Su-Yi Tsai, Mona Khalaj, Ting Zhou, Ryan M. Samuel, Tuo Zhang, Francis A. Ortega, Miriam Gordillo, Dorota Moroziewicz, Daniel Paull, Scott A. Noggle, Jenny Zhaoying Xiang, Lorenz Studer, David J. Christini, Geoffrey S. Pitt, Todd Evans, Shuibing Chen   +21 more
doaj   +1 more source

The Rigor and Transparency Index Quality Metric for Assessing Biological and Medical Science Methods

iScience, 2020
Summary: The reproducibility crisis is a multifaceted problem involving ingrained practices within the scientific community. Fortunately, some causes are addressed by the author's adherence to rigor and reproducibility criteria, implemented via ...
Joe Menke, Martijn Roelandse, Burak Ozyurt, Maryann Martone, Anita Bandrowski   +4 more
doaj   +1 more source

Low-temperature resin embedding of the whole brain for various precise structures dissection

iScience, 2023
Summary: Resin embedding combined with ultra-thin sectioning has been widely used in microscopic and electron imaging to acquire precise structural information of biological tissues.
Jiaojiao Tian, Yingying Chen, Tao Jiang, Xueyan Jia, Hui Gong, Xiangning Li   +5 more
doaj   +1 more source

A realistic transfer method reveals low risk of SARS-CoV-2 transmission via contaminated euro coins and banknotes

iScience, 2021
Summary: The current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has created a significant threat to global health. While respiratory aerosols or droplets are considered as the main route of human-to-human transmission ...
Daniel Todt, Toni Luise Meister, Barbora Tamele, John Howes, Dajana Paulmann, Britta Becker, Florian H. Brill, Mark Wind, Jack Schijven, Natalie Heinen, Volker Kinast, Baxolele Mhlekude, Christine Goffinet, Adalbert Krawczyk, Jörg Steinmann, Stephanie Pfaender, Yannick Brüggemann, Eike Steinmann   +17 more
doaj   +1 more source

Robust induction of functional astrocytes using NGN2 expression in human pluripotent stem cells

iScience, 2023
Summary: Emerging evidence of species divergent features of astrocytes coupled with the relative inaccessibility of human brain tissue underscore the utility of human pluripotent stem cell (hPSC) technologies for the generation and study of human ...
Martin H. Berryer, Matthew Tegtmeyer, Loïc Binan, Vera Valakh, Anna Nathanson, Darina Trendafilova, Ethan Crouse, Jenny A. Klein, Daniel Meyer, Olli Pietiläinen, Francesca Rapino, Samouil L. Farhi, Lee L. Rubin, Steven A. McCarroll, Ralda Nehme, Lindy E. Barrett   +15 more
doaj   +1 more source

Antibody-based sex determination of human skeletal remains

iScience, 2023
Summary: Assignment of biological sex to skeletal remains is critical in the accurate reconstruction of the past. Analysis of sex-chromosome encoded AMELX and AMELY peptides from the enamel protein amelogenin underpins a minimally destructive mass ...
Barry Shaw, Sophie Foggin, Petter Hamilton-Stanley, Andy Barlow, Catriona Pickard, Linda Fibiger, Neil Oldham, Patrick Tighe, Lisette M. Kootker, Sarah Schrader, Rob Layfield   +10 more
doaj   +1 more source

Maximum tree lifespans derived from public-domain dendrochronological data

iScience, 2023
Summary: The public-domain International Tree-Ring Data Bank (ITRDB) is an under-utilized dataset to improve existing estimates of global tree longevity.
Franco Biondi, David M. Meko, Gianluca Piovesan   +2 more
doaj   +1 more source

Visualization of PS/γ-Secretase Activity in Living Cells

iScience, 2020
Summary: A change in Presenilin (PS)/γ-secretase activity is linked to essential biological events as well as to the progression of many diseases. However, not much is known about how PS/γ-secretase activity is spatiotemporally regulated in cells. One of
Masato Maesako, Nicole M. Sekula, Anna Aristarkhova, Polina Feschenko, Lauren C. Anderson, Oksana Berezovska   +5 more
doaj   +1 more source