Results 111 to 120 of about 252,850 (254)

Characterization and Protective Efficacy of a Salmonella Typhimurium ATCC 14028 sptP Mutant as a Live Attenuated Vaccine Candidate

Vaccines
Background: Salmonella Typhimurium poses a substantial health risk to both humans and animals. This study evaluated the potential of using the Salmonella Typhimurium ΔsptP mutant as a live-attenuated vaccine candidate by constructing it through ...
Nanlong Zhou, Yonghui Ding, Ting He, Yuling Sun, Hongfang Chen, Meiling Huang, Tiansen Li   +6 more
doaj   +1 more source

Sirt3 Genetically Engineered Apoptotic Bodies Alleviate Skeletal Aging by Limiting Aggravated NLRP3 Inflammasome Activation of Senescent Macrophages

Advanced Science, EarlyView.
The senescence of macrophages leads to a decrease in the expression of Sirtuin3 (Sirt3), which in turn aggravates the NLRP3 inflammasome activation and IL‐1β secretion, thereby promoting the aging‐related osteoporosis. Sirt3‐enriched apoptotic bodies (ABs‐Sirt3) generated through genetic editing approaches can alleviate skeletal aging by targeting ...
Yanglin Wu, Shifeng Ling, Jiayi Mao, Hongyi Wang, Bo Wang, Zhenjia Che, Wenguo Cui, Ming Cai   +7 more
wiley   +1 more source

Generation and Characterization of Three Novel Mouse Mutant Strains Susceptible to Audiogenic Seizures

Cells
The mechanisms of epileptogenesis after brain injury, ischemic stroke, or brain tumors have been extensively studied. As a result, many effective antiseizure drugs have been developed.
Elena G. Varlamova, Vera P. Kuldaeva, Natalia N. Mitina, Maria S. Gavrish, Elena V. Kondakova, Victor S. Tarabykin, Alexei A. Babaev, Egor A. Turovsky   +7 more
doaj   +1 more source

pFAR plasmids: New Eukaryotic Expression Vectors for Gene Therapy, devoid of Antibiotic Resistance Markers [PDF]

, 2008
Efficient production of eukaryotic expression vectors requires the selection of plasmid-containing bacteria. To avoid the risk of dissemination of antibiotic resistance markers, we developed a new system to produce a family of plasmids Free of Antibiotic
Corinne Marie, Daniel Scherman, Gaelle Vandermeulen, Magali Richard, Mickael Quiviger, Veronique Preat   +5 more
core   +1 more source

DMAP1 Deficiency Suppresses Lung Cancer Progression by Destabilizing Replication Fork and Activating IFN Signaling‐Mediated Anti‐tumor Immunity

Advanced Science, EarlyView.
Lung cancer remains the leading cause of cancer‐related death. We investigated the role of the epigenetic regulator DMAP1 in NSCLC and found that its loss induces replication stress and DNA damage. This in turn activates type I IFN signaling via the cGAS–STING pathway and transcriptional ISG de‐repression, enhancing anti‐tumor immune responses ...
Kan Huang, Xi Dai, Shuaihu Li, Yingxue Chen, Yaxin Yu, Lin Wang, Kun Liu, Shuhan Lyu, Chongyang Li, Yihua Sun, Fei Li   +10 more
wiley   +1 more source

Organ‐Specific and Conserved Regulatory Logic Orchestrates Gene Expression in the Embryonic Mesothelium

Advanced Science, EarlyView.
Integrated multi‐omic profiling maps the gene‐regulatory landscape of the coelomic mesothelium across heart, lung, and pancreas. A cardiac‐restricted regulatory program is uncovered in which TBX20 activates heart mesothelial (epicardial) cis‐regulatory elements, while MAF emerges as a conserved regulator of mesothelial identity.
Quang Minh Dang, Nicola Smart, Andia Nicole Redpath, Joaquim Miguel Vieira   +3 more
wiley   +1 more source

Heterozygous Spink1 Deficiency Promotes Trypsin-dependent Chronic Pancreatitis in MiceSummary

Cellular and Molecular Gastroenterology and Hepatology
Background & Aims: Heterozygous SPINK1 mutations are strong risk factors for chronic pancreatitis in humans, yet heterozygous disruption of mouse Spink1 yielded no pancreatic phenotype.
Alexandra Demcsák, Miklós Sahin-Tóth
doaj   +1 more source

Intracellular Aβ42 Sequestration by a Serine Protease Mitigates Neurotoxicity in a Drosophila Alzheimer's Disease Model

Advanced Science, EarlyView.
Emerging evidence suggests that intraneuronal Aβ accumulation represents an early pathogenic event in Alzheimer's disease (AD). Using Drosophila AD model, this study shows that a nonsecreted serine protease Yip7 physically interacts with Aβ. This causes intraneuronal Aβ accumulation but surprisingly reduces the associated neurotoxicity, arguing that ...
Jingyun Su, Meng Yang, Xinfeng Wang, Pa Wu, Zongzhao Zhai   +4 more
wiley   +1 more source

Cell wall glycosyltransferase of Streptococcus mutans impacts its dissemination to murine organs

Infection and Immunity
Streptococcus mutans, a cariogenic bacterium in humans, is associated with systemic disorders. Its cariogenic factors include glucosyltransferases (GTFs) and the glycosyltransferase rhamnose–glucose polysaccharide I (RgpI), which is involved in cell wall
Tomomi Hashizume-Takizawa, Taiki Ando, Ayaka Urakawa, Kazuhiro Aoki, Hedenobu Senpuku   +4 more
doaj   +1 more source

Coordination of Cyanobacterial Nitrate Assimilation and Photosynthesis by a Novel PsbO‐Interacting Protein PirN

Advanced Science, EarlyView.
PirN was identified as a previously uncharacterized PsbO‐interacting protein specifically induced in nitrate‐grown cyanobacteria. Loss of PirN leads to coordinated downregulation of PsbO and the nitrate reductase NarB, causing decreased PSII activity and impaired growth under nitrate conditions. Complementation with either PsbO or NarB largely restores
Chengcheng Huang, Zhen Xiao, Haitao Ge, Gaojie Wang, Jinghui Dong, Yan Wang, Hang Yang, Xing Wang, Hui Gao, Zhongshu Wang, Huanling Yang, Yuanya Zhang, Xiahe Huang, Wu Xu, Weimin Ma, Wenqiang Yang, Yingchun Wang   +16 more
wiley   +1 more source