Results 71 to 80 of about 81,651 (208)

Unveiling the Taxonomic Diversity and Unprecedented Biosynthetic Treasure of the Phylum Myxococcota

Advanced Science, EarlyView.
Natural products remain vital sources of therapeutics, and the phylum Myxococcota constitutes an especially rich reservoir for them. Based on decades of microbiological efforts, we present 154 new Myxococcota genomes and revise taxonomy quadrupling the number of families and genera.
Amay Ajaykumar Agrawal, Ronald Garcia, Guangyi Chen, Chantal D. Bader, Peter Sullivan, Alexander Popoff, Daniel Krug, Maja Hunter, Sebastian Walesch, Emilia Oueis, Christine Frank, Haowen Zhao, Joachim J. Hug, Sebastian Keller, Pascal Hirsch, Azat Tagirdzhanov, Tatiana Malygina, Mary Victory E. Gutierrez, Alexey Gurevich, Judith Boldt, Boyke Bunk, Jörg Overmann, Ulrich Nübel, Andreas Keller, Olga V. Kalinina, Rolf Müller   +25 more
wiley   +1 more source

Continuous Evolution System Based on Toxin–Antitoxin System Combined with Base Deaminase Accelerates the In Vivo Modification of Target Proteins

Advanced Science, EarlyView.
The promoted Escherichia coli‐assisted continuous evolution (PEACE) system operates through a stringent three step genetic circuit: target gene mutation via a deaminase–T7 RNA polymerase (T7 RNAP) fusion, coupling variant function to antitoxin expression, and growth based selection of surviving cells.
Xinyu Zhang, Zhanzhi Liu, Ying Xu, Mingjing Sun, Guangzhen Zhou, Deming Rao, Xiaoqian Chen, Sheng Wang, Zhigang Li, Yan Huang, Jing Wu   +10 more
wiley   +1 more source

Rapid Multiplex Antimicrobial Resistance Profiling and Bacterial Identification by LAP‐MALDI Mass Spectrometry Biotyping

Advanced Science, EarlyView.
Liquid atmospheric pressure matrix‐assisted laser desorption/ionization (LAP‐MALDI) mass spectrometry (MS) provides multiplexed biotyping of microbial cultures using a fast and simple workflow. This is demonstrated for clinically relevant bacteria, resulting in accurate antimicrobial resistance (AMR) detection, speciation by MS/MS sequencing and ...
Lily R. Adair, Shabnam Iyer, Ian M. Jones, Rainer Cramer   +3 more
wiley   +1 more source

Ion‐Driven Regulation of Ca2+ Flow by Ti/Zn Composite Activates ERK/MAPK Signaling and Maintains Mitochondrial Homeostasis to Promote Macrophage‐Mediated Bone Regeneration

Advanced Science, EarlyView.
A 3D‐printed interpenetrating Ti/Zn composite enables sustained Zn2+ release to regulate intracellular Ca2+ redistribution, preserve mitochondrial homeostasis, activate ERK/MAPK signaling, and promote M2 macrophage polarization, thereby enhancing osteoimmune‐mediated bone regeneration and peri‐implant osseointegration.
Wanyi Huang, Qiyue Zhang, Yueyang Qiu, Haocheng Suo, Zengqian Liu, Bing Bai, Qiang Wang, Qing Zhou   +7 more
wiley   +1 more source

Artificial Intelligence‐Guided Phenotypic Drug Repurposing Against Streptococcus pneumoniae

Advanced Science, EarlyView.
Integrated artificial intelligence (AI) ensembles identify highly potent repurposed drugs against Streptococcus pneumoniae. Prospective virtual screening of 6747 drugs uncovers nine active candidates, including thiostrepton. This drug exhibits low picomolar potency and bypasses established resistance in multidrug‐resistant strains, demonstrating an ...
Joshua S. Fitch, Muhammad D. Ariadi, Min Jung Kwun, Leonie Howells, Saiveth Hernandez‐Hernandez, Nicholas J. Croucher, Pedro J. Ballester   +6 more
wiley   +1 more source

A pH‐Switchable Cerium Single‐Atom Enzyme for Peri‐Implantitis Therapy via JAK‐STAT Signaling Axis‐Mediated Macrophage Reprogramming

Advanced Science, EarlyView.
A pH‐switchable cerium single‐atom enzyme is developed for stage‐specific peri‐implantitis therapy. Acidic conditions trigger antibacterial peroxidase‐like activity, whereas neutral conditions activate an antioxidant superoxide dismutase ‐ and catalase ‐like enzymatic relay. This microenvironment‐adaptive catalytic strategy suppresses the IL‐6/JAK‐STAT
Xiaomin Xia, Haojie Wu, Xingyun Li, Lining Liu, Kaiqing Song, Jinpei Cui, Jie Liu, Jiaxuan Yin, Emtiaz Ahmed, Yusuke Yamauchi, Xue Li   +10 more
wiley   +1 more source

A Microcapsule‐Integrated smRandom‐seq Platform Enables Fixation‐Free Single Microbe RNA Sequencing

Advanced Science, EarlyView.
We developed a microcapsule‐integrated high‐throughput fixation‐free single‐microbe RNA‐seq workflow in which microbes are encapsulated in aqueous two‐phase microcapsules for physical confinement of nucleic acids. This platform enables co‐encapsulation with barcoded beads for sequencing and reveals that fixation‐free processing enhances transcriptomic ...
Mengdi Song, Yuting Wang, Zhaolun Wang, Shunji Zhang, Zhengmin Tang, Yongcheng Wang   +5 more
wiley   +1 more source

Validation of Ohmic Heating Pilot Plant for Vitamin C Retention and E. coli Surrogate Inactivation on Strawberry Nectar

International Journal of Food Science
This study validates the performance of a pilot-scale ohmic heating plant for vitamin C retention and Escherichia coli surrogate inactivation in strawberry nectar, based on thermal kinetics determined using a thermoresistometer.
Dario J. Pavon-Vargas, Vincenzo Alfonsi, Stephane Georgé, Mario Gozzi, Sara Rainieri, Luca Cattani   +5 more
doaj   +1 more source

Repurposing a Small Molecule Plant Hormone as a Tunable ON‐Switch for CAR‐T Cell Immunotherapy

Advanced Science, EarlyView.
By engineering a receptor system integrating the plant auxin receptor AFB1 with its co‐receptor IAA7, we enable ligand‐dependent interactions triggered by the plant hormone auxins. This design allows rapid, reversible, and dose‐dependent T cell activation, resulting in potent cytotoxicity against B‐cell lymphoma in vitro and in vivo.
Hongxiang Zeng, Lei Zheng, Rongkun Tao, Sirui Zheng, Yanke Zhong, Yongxia Niu, Peng Zhang, Xiang Ma, Chen Yang, Min Zhang, Fuyu Duan, Jing Yang, Xuan Wei, Xiang Zhou, Zhenbiao Yang, Zhaoxi Sun, Qizhou Lian   +16 more
wiley   +1 more source

Dual‐Responsive Hydrogels Integrate Bioelectrical Stimulation and Piezo‐Photocatalysis for Regenerative Treatment of Infected Wounds

Advanced Science, EarlyView.
A wireless, dual‐responsive hydrogel (PVA/(PVDF‐TrFE)/BiOClBr@PDA) is developed to promote efficient regeneration of infected wounds by integrating piezoelectric stimulation with piezo‐photocatalytic pathways. Under simultaneous light and ultrasonic stimulation, the hydrogel generates therapeutic electrical cues and reactive oxygen species, disrupting ...
Baoying Dai, Wenxuan Qie, Xiaoye Li, Jingben Zheng, Ao He, Heng Dong, Jiangjiang Yan, Hao Zhang, Ziheng Pan, Hang Yin, Chuyi Zhou, Rui Kong, Hao Wang, Binghan Dai, Yannan Xie, Zhiqun Lin   +15 more
wiley   +1 more source