Results 211 to 220 of about 10,401 (257)

Microbial Lipase: Structure and Production

2020
In this chapter, the authors describe the gene cloning, structures, and production mechanisms of microbial lipases and their production by recombinant microbes. The total RNA was extracted with 4 M guanidine thiocyanate solution from log-phase cells showing the maximum rate of specific lipase production. The primary structures of bacterial lipases were
Y, Shimada, A, Sugihara, Y, Tominaga
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The realm of microbial lipases in biotechnology

Biotechnology and Applied Biochemistry, 1999
In this review, a comprehensive and illustrious survey is made of the applied aspects of microbial lipases in modern biotechnological practices. Lipases are the most versatile biocatalyst and bring about a range of bioconversion reactions such as hydrolysis, interesterification, esterification, alcoholysis, acidolysis and aminolysis.
A, Pandey, S, Benjamin, C R, Soccol, P, Nigam, N, Krieger, V T, Soccol   +5 more
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Fluorescent organophosphonates as inhibitors of microbial lipases

Chemistry and Physics of Lipids, 2003
Short- and long-chain 1-O-alkyl-2-acylaminodeoxyglycero- and alkoxy-alkylphosphonic acid p-nitrophenyl esters were synthesized as inhibitors for analytical and mechanistic studies on lipolytic enzymes. The respective compounds contain perylene or nitrobenzoxadiazole as reporter fluorophores covalently bound to the omega-ends of the respective 2 ...
Olga Oskolkova, Robert Saf, Albin Hermetter   +2 more
exaly   +3 more sources

Utilization of Methyloleate in Production of Microbial Lipase

Applied Biochemistry and Biotechnology, 2005
In this article, we report the development and optimization of an industrial culture medium for the production of extracellular lipase in the yeast Yarrowia lipolytica. Until now olive oil in combination with glucose was used as the carbon source and inducer for the production of lipase.
Destain, Jacqueline, Fickers, Patrick, Weekers, F., Moreau, Benoit, Thonart, Philippe   +4 more
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Inhibition of pancreatic and microbial lipases by proteins

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1984
We have compared the effect of several proteins, including melittin, beta-lactoglobulin A, serum albumin, ovalbumin and myoglobin, on the hydrolysis of tributyrin and triolein by lipases from various origins. All proteins tested inactivate pancreatic lipase in absence of colipase and bile salt.
Y, Gargouri, R, Julien, A, Sugihara, R, Verger, L, Sarda   +4 more
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Stereoselectivity of Microbial Lipases

European Journal of Biochemistry, 1995
In the present study, the stereoselectivity of purified lipases from Candida rugosa, Chromobacterium viscosum, Pseudomonas species and Rhizopus arrhizus towards triacylglycerols in comparison to various structural analogs were investigated. Different triacylglycerol analogs with distinct polarities at position sn‐2 of the glycerol backbone (1,3‐diacy1 ...
P, Stadler, A, Kovac, L, Haalck, F, Spener, F, Paltauf   +4 more
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Overproduction of microbial lipids and lipases

Folia Microbiologica, 1991
The nomenclature of simple and complex lipids, and their analysis are introduced and the overproduction of lipids and lipases are discussed.
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Recombinant microbial lipases for biotechnological applications

Bioorganic & Medicinal Chemistry, 1999
Lipases, mainly of microbial origin, represent the most widely used class of enzymes in biotechnological applications and organic chemistry. Modern methods of genetic engineering combined with an increasing knowledge of structure and function will allow further adaptation to industrial needs and exploration of novel applications.
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Positional specificity of microbial lipases

Journal of Fermentation and Bioengineering, 1991
The positional specificity of microbial lipases was studied by a method based on the measurement of diglyceride (DG) isomers in their reaction products using a TLC-autodetector and calculation of a positional specificity index defined as follows: PSI = [1, 2(2, 3)DG − 1, 3DG × 2] × 100/[1, 2(2,3)DG + 1,3DG × 2].
Miwa Matori, Takehiko Asahara, Yasuhide Ota   +2 more
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Industrial application of microbial lipases: A review

Journal of the American Oil Chemists' Society, 1974
AbstractCommercial microbial lipases have been used in dairy and other food processes, and lipases produced in situ by microorganisms are important in making foods palatable and acceptable. Microbial lipases have been used in detergents, pharmaceuticals, cosmetics, leather processing, production of aliphatic acids, and in the treatment of domestic and ...
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