Results 31 to 40 of about 547,231 (309)

Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2

Nature Communications, 2021
Hoshino et al., engineer a human virus receptor, hACE2, and demonstrate its potential for overcoming SARS-CoV-2 mutations that otherwise hinder therapeutic interventions.
Yusuke Higuchi, Tatsuya Suzuki, Takao Arimori, Nariko Ikemura, Emiko Mihara, Yuhei Kirita, Eriko Ohgitani, Osam Mazda, Daisuke Motooka, Shota Nakamura, Yusuke Sakai, Yumi Itoh, Fuminori Sugihara, Yoshiharu Matsuura, Satoaki Matoba, Toru Okamoto, Junichi Takagi, Atsushi Hoshino   +17 more
doaj   +1 more source

Nudix proteins affecting microbial pathogenesis

Microbiology, 2020
Nudix proteins catalyse hydrolysis of pyrophosphate bonds in a variety of substrates and are ubiquitous in all domains of life. Their widespread presence and broad substrate specificity suggest that they have important cellular functions. In this review, we summarize the state of knowledge on microbial Nudix proteins involved in pathogenesis.
Elzbieta Kraszewska, Joanna Drabinska
openaire   +2 more sources

Mycobacterium tuberculosis CarD, an essential global transcriptional regulator forms amyloid-like fibrils

Scientific Reports, 2018
CarD is an essential global transcription regulator from Mycobacterium tuberculosis (Mtb) that binds RNA polymerase and activates transcription by stabilizing the transcription initiation complex.
Gundeep Kaur, Soni Kaundal, Srajan Kapoor, Jonathan M. Grimes, Juha T. Huiskonen, Krishan Gopal Thakur   +5 more
doaj   +1 more source

Isolation and Characterization of Strain Exiguobacterium sp. KRL4, a Producer of Bioactive Secondary Metabolites from a Tibetan Glacier

Microorganisms, 2021
Extremophilic microorganisms represent a unique source of novel natural products. Among them, cold adapted bacteria and particularly alpine microorganisms are still underexplored.
Pietro Tedesco, Fortunato Palma Esposito, Antonio Masino, Giovanni Andrea Vitale, Emiliana Tortorella, Annarita Poli, Barbara Nicolaus, Leonardo Joaquim van Zyl, Marla Trindade, Donatella de Pascale   +9 more
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Harnessing the evolutionary information on oxygen binding proteins through Support Vector Machines based modules

BMC Research Notes, 2018
Objectives The arrival of free oxygen on the globe, aerobic life is becoming possible. However, it has become very clear that the oxygen binding proteins are widespread in the biosphere and are found in all groups of organisms, including prokaryotes ...
Selvaraj Muthukrishnan, Munish Puri
doaj   +1 more source

Engraftment of Bacteria after Fecal Microbiota Transplantation Is Dependent on Both Frequency of Dosing and Duration of Preparative Antibiotic Regimen

Microorganisms, 2021
The gut microbiota has emerged as a key mediator of human physiology, and germ-free mice have been essential in demonstrating a role for the microbiome in disease.
Vancheswaran Gopalakrishnan, Elizabeth Ashley Dozier, Matthew S. Glover, Steven Novick, Michael Ford, Christopher Morehouse, Paul Warrener, Carolina Caceres, Sonja Hess, Bret R. Sellman, Taylor S. Cohen   +10 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Fatty Acid and Amino Acid Profiles of Seven Edible Insects: Focus on Lipid Class Composition and Protein Conversion Factors

Foods, 2023
This study is based on the fatty acid and amino acid profiles of seven edible insect species: Acheta domesticus, Alphitobius diaperinus, Blaptica dubia, Galleria mellonella, Locusta migratoria, Tenebrio molitor, and Zophobas morio. The aim of the present
Cristina Perez-Santaescolastica, Ilse de Pril, Ilse van de Voorde, Ilse Fraeye   +3 more
doaj   +1 more source

Microbial expression systems for membrane proteins [PDF]

Methods, 2018
Despite many high-profile successes, recombinant membrane protein production remains a technical challenge; it is still the case that many fewer membrane protein structures have been published than those of soluble proteins. However, progress is being made because empirical methods have been developed to produce the required quantity and quality of ...
Dilworth, Marvin V.; Piel, Mathilde S.; Bettaney, Kim E.; Ma, Pikyee; Luo, Ji; Sharples, David; Poyner, David R.; Gross, Stephane R.; Moncoq, Karine; Henderson, Peter J. F.; Miroux, Bruno; Bill, Roslyn M.   +12 more
openaire   +3 more sources