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Immunoguided Microdissection Techniques [PDF]
, 2011 Over the past 15 years, laser-based microdissection has improved the precision by which scientists can procure cells of interest from a heterogeneous tissue section. However, for studies that require a large amount of material (e.g., proteomics) or for cells that are scattered and difficult to identify by standard histological stains, an immunostain ...
Michael A, Tangrea +5 more
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Nephrology, 2019 Laser microdissection (LMD) and liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) enable clinicians to analyse proteins from tissue sections.
Naoto Kawata +8 more
semanticscholar +1 more source
Microdissected Prefabricated Flap: An Evolution in Flap Prefabrication
Archives of Plastic Surgery, 2016 When traditional flap techniques are not feasible, we apply flap prefabrication, which is more complicated and sophisticated but supplies large and thin flaps.
Süleyman Taş
doaj +1 more source
Analytical Chemistry, 2018 Proteome profiling of circulating tumor cells (CTCs) can provide crucial insight into disease progression and the role of CTCs in tumor metastasis. We describe an integrated workflow to measure global protein expression in 1-5 spiked CTCs enriched from ...
Ying Zhu +6 more
semanticscholar +1 more source
Genetics Selection Evolution, 2003 A pericentric inversion of chromosome 4 in a boar, as well as a case of (2q-;5p+) translocation mosaicism in a bull were analysed by chromosome painting using probes generated by conventional microdissection.
Yerle Martine, Ducos Alain, Pinton Alain
doaj +1 more source
Transcriptome Analysis of Intrusively Growing Flax Fibers Isolated by Laser Microdissection
Scientific Reports, 2018 The intrusive growth, a type of plant cell elongation occurring in the depths of plant tissues, is characterized by the invasion of a growing cell between its neighbours due to a higher rate of elongation. In order to reveal the largely unknown molecular
T. Gorshkova +5 more
semanticscholar +1 more source
Nature Protocols, 2006 Deciphering the cellular and molecular interactions that drive disease within the tissue microenvironment holds promise for discovering drug targets of the future. In order to recapitulate the in vivo interactions thorough molecular analysis, one must be able to analyze specific cell populations within the context of their heterogeneous tissue ...
Virginia, Espina +8 more
openaire +4 more sources
SurgeriesThe anterior cruciate ligament (ACL) is crucial for knee stability and is often injured in sports, leading to significant issues like degenerative changes and meniscal tears.
Ondar Artysh Vyacheslavovich +8 more
doaj +1 more source
BMC Evolutionary Biology, 2011 Background The Erythrinidae fish family is characterized by a large variation with respect to diploid chromosome numbers and sex-determining systems among its species, including two multiple X1X2Y sex systems in Hoplias malabaricus and Erythrinus ...
Trifonov Vladimir +6 more
doaj +1 more source
Advanced Science, EarlyView.An orthogonal ionic‐liquid extraction (Orth‐iEA) enables selective isolation of amyloid fibrils. TMGBF4 disrupts hydrogen‐bonded β‐sheet networks to solubilize amyloid aggregates, whereas C12ImCl interacts with hydrophobic regions of non‐amyloid proteins.
Shiying Zheng +10 more
wiley +1 more source