Results 101 to 110 of about 16,295 (141)

Purification of microvilli and an analysis of the protein components of the microfilament core bundle

Experimental Cell Research, 1978
Abstract A fast and convenient method for the purification of microvilli from chicken intestinal brush borders is described. The microvilli appear morphologically very similar to those found on intact brush borders. Removal of the microvillus membrane from the microvilli by Triton X-100 treatment reveals compact bundles of microfilaments with small ...
A, Bretscher, K, Weber
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Microfilaments from amoeba proteins

Experimental Cell Research, 1971
Summary Bundles of microfilaments have been obtained from a fraction of homogenised amoebae cells after the addition of EDTA. These bundles are made up of many single microfilaments 30–40 A in diameter. An attempt has been made to obtain the protein subunit of these microfilaments. In the detergent Sarkosyl, a subunit of sedimentation constant 2.0×10−
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Microfilament bundles, lets protein and growth control in somatic cell hybrids

Journal of Cell Science, 1978
ABSTRACT Hybrid cell lines between normal rat embryo fibroblasts and TA3B mouse tumour cells, or between TA3B and BI hamster sarcoma cells, have been examined for the expression of the cell surface large external-transformation-sensitive (LETS) protein and the organization of microfilament bundles.
C J, Marshall, K C, Humphryes, R E, Pollack   +2 more
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Anti-microfilament IgG Antibodies in Normal Adults and in Patients with Autoimmune Diseases: Immunofluorescence and Immunoblotting Analysis of 201 Subjects Reveals Polyreactivity with Microfilament-Associated Proteins

Clinical Immunology and Immunopathology, 1995
We have investigated IgG antibodies to microfilament-associated proteins in sera from 29 normal controls and from 172 patients with autoimmune diseases such as rheumatoid arthritis (n = 45), systemic lupus erythematosus (n = 43), polymyositis/dermatomyositis (n = 36), systemic sclerosis (n = 35), and autoimmune chronic active hepatitis (CAH, n = 13 ...
Denis Girard, Jean-Luc Sénécal
exaly   +3 more sources

The Roles of Microfilament-Associated Proteins, Drebrins, in Brain Morphogenesis: A Review

Journal of Biochemistry, 1995
The cytoskeleton has been suggested to be one of the important endogenous factors that control neuronal morphogenesis. Analysis of the developmental changes in the protein composition of the brain led to the discovery of novel developmentally regulated actin-binding proteins, drebrins.
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Localization and organization of microfilaments and related proteins in normal and virus‐transformed cells

Journal of Supramolecular Structure, 1976
AbstractThe localization and organization of actin‐like microfilaments in normal, SV‐40 and adenovirus transformed cells are determined by the coordinated use of light optical, electron optical and biochemical techniques. In adenovirus‐type 5 transformed hamster embryo cells, microfilament meshworks appear to be the predominant organizational form of ...
R D, Goldman, M J, Yerna, J A, Schloss
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Phalloidin shift on velocity sedimentation sucrose gradient centrifugation for identification of microfilament-associated proteins

Experimental Cell Research, 1985
Velocity sedimentation by sucrose density gradient centrifugation has been used to characterize ascites microvillar microfilament cores and to identify microfilament-associated proteins. Fluoride, calcium, phalloidin and chemical cross-linking treatments of microvilli during Triton X-100 extractions increase the sedimentation rate of the microfilament ...
Coralie A Carothers Carraway, C A Carraway   +1 more
exaly   +3 more sources

Motor proteins regulate force interactions between microtubules and microfilaments in the axon

Nature Cell Biology, 2000
It has long been known that microtubule depletion causes axons to retract in a microfilament-dependent manner, although it was not known whether these effects are the result of motor-generated forces on these cytoskeletal elements. Here we show that inhibition of the motor activity of cytoplasmic dynein causes the axon to retract in the presence of ...
F J, Ahmad, J, Hughey, T, Wittmann, A, Hyman, M, Greaser, P W, Baas   +5 more
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Early changes in the distribution and organization of microfilament proteins during cell transformation

Cell, 1981
During the onset of transformation, Rous sarcoma virus-infected cells undergo characteristic morphological changes that reflect the biochemical events induced by the viral src gene. Temperature downshift experiments using chick embryo cells infected with transformation-defective temperature-sensitive viral mutants have shown two major morphological ...
C B, Boschek, B M, Jockusch, R R, Friis, R, Back, E, Grundmann, H, Bauer   +5 more
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Antibodies against vertebrate microfilament proteins in the analysis of cellular motility and adhesion

Journal of Cell Science, 1991
ABSTRACT Microinjection of specific antibodies can be an alternative and a supplement to genetic engineering in dissecting the function of individual cytoskeletal components. In this report, we describe some of the requirements for using this technique, its potential application in conjunction with morphological and biochemical analyses,
B M, Jockusch, B, Zurek, R, Zahn, A, Westmeyer, A, Füchtbauer   +4 more
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