Results 1 to 10 of about 77,276 (354)

Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle [version 1; referees: 2 approved, 1 approved with reservations]

open access: yesF1000Research, 2016
Higher vertebrates express six different highly conserved actin isoforms that can be classified in three subgroups: 1) sarcomeric actins, α-skeletal (α-SKA) and α-cardiac (α-CAA), 2) smooth muscle actins (SMAs), α-SMA and γ-SMA, and 3) cytoplasmic actins
Christine Chaponnier, Giulio Gabbiani
doaj   +1 more source

Molecular characterization of three muscle alpha actin genes in mud loach (Misgurnus mizolepis; Cypriniformes)

open access: yesFisheries and Aquatic Sciences, 2017
Background Teleosts represent unique features in the regulation of muscle development and growth, with a great deal of myogenic plasticity. Muscle actins are major components to compose muscle fibers, and they play essential roles in cellular mobility ...
Sang Yoon Lee   +2 more
doaj   +1 more source

Monoclonal antibodies against muscle actin isoforms: epitope identification and analysis of isoform expression by immunoblot and immunostaining in normal and regenerating skeletal muscle [version 2; referees: 3 approved]

open access: yesF1000Research, 2016
Higher vertebrates (mammals and birds) express six different highly conserved actin isoforms that can be classified in three subgroups: 1) sarcomeric actins, α-skeletal (α-SKA) and α-cardiac (α-CAA), 2) smooth muscle actins (SMAs), α-SMA and γ-SMA, and 3)
Christine Chaponnier, Giulio Gabbiani
doaj   +1 more source

Stability domains of actin genes and genomic evolution

open access: yes, 2007
In eukaryotic genes the protein coding sequence is split into several fragments, the exons, separated by non-coding DNA stretches, the introns. Prokaryotes do not have introns in their genome.
A. Dkhissi   +9 more
core   +1 more source

Essential nucleotide- and protein-dependent functions of Actb/β-actin [PDF]

open access: yes, 2018
The highly similar cytoplasmic β- and γ-actins differ by only four functionally similar amino acids, yet previous in vitro and in vivo data suggest that they support unique functions due to striking phenotypic differences between Actb and Actg1 null ...
Chamberlain, Christopher M.   +8 more
core   +1 more source

The mother of all actins?

open access: yeseLife, 2016
New insights into the structure of filaments made of crenactin, a homolog of actin found in archaea, shed light on how the cytoskeleton might have evolved.
Felipe Merino, Stefan Raunser
doaj   +1 more source

A method for rapidly screening functionality of actin mutants and tagged actins [PDF]

open access: yesBiological Procedures Online, 2004
Recombinant production and biochemical analysis of actin mutants has been hampered by the fact that actin has an absolute requirement for the eukaryotic chaperone CCT to reach its native state.
Rommelaere Heidi   +4 more
doaj   +1 more source

Relative importance of βcyto- and γcyto-actin in primary mouse embryonic fibroblasts [PDF]

open access: yes, 2017
The highly homologous β (βcyto) and γ (γcyto) cytoplasmic actins are hypothesized to carry out both redundant and unique essential functions, but studies using targeted gene knockout and siRNA-mediated transcript knockdown to examine βcyto- and γcyto ...
Chamberlain, Christopher M.   +5 more
core   +1 more source

A novel microfluidic flow-cytometry for counting numbers of single-cell β-actins

open access: yesNanotechnology and Precision Engineering, 2020
As a house keeping protein with stable expressions, β-actin is used as a loading control in normalization of western blotting. However, the actual numbers of β-actins at the single-cell level remain elusive.
Lixing Liu   +6 more
doaj   +1 more source

Collective force generated by multiple biofilaments can exceed the sum of forces due to individual ones

open access: yes, 2014
Collective dynamics and force generation by cytoskeletal filaments are crucial in many cellular processes. Investigating growth dynamics of a bundle of N independent cytoskeletal filaments pushing against a wall, we show that chemical switching (ATP/GTP ...
Das, Dibyendu   +2 more
core   +2 more sources

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