Results 231 to 240 of about 25,497 (250)

Disruption of endothelial actin microfilaments by protein kinase C inhibitors

Microvascular Research, 1992
In this study, we report that the isoquinolinesulfonamide inhibitors of protein kinase C (PKC), H-7 [1-(5-isoquinolinesulfonyl)-2-methylpiperazine] and its related derivatives H-8 and HA-1004, in addition to staurosporine cause depletion and reorganization of microfilament bundles of porcine aortic endothelial cells in both low-density and confluent ...
J C, Yu, A I, Gotlieb
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Microfilament bundles, lets protein and growth control in somatic cell hybrids

Journal of Cell Science, 1978
ABSTRACT Hybrid cell lines between normal rat embryo fibroblasts and TA3B mouse tumour cells, or between TA3B and BI hamster sarcoma cells, have been examined for the expression of the cell surface large external-transformation-sensitive (LETS) protein and the organization of microfilament bundles.
C J, Marshall, K C, Humphryes, R E, Pollack   +2 more
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Microfilaments from amoeba proteins

Experimental Cell Research, 1971
Summary Bundles of microfilaments have been obtained from a fraction of homogenised amoebae cells after the addition of EDTA. These bundles are made up of many single microfilaments 30–40 A in diameter. An attempt has been made to obtain the protein subunit of these microfilaments. In the detergent Sarkosyl, a subunit of sedimentation constant 2.0×10−
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Motor proteins regulate force interactions between microtubules and microfilaments in the axon

Nature Cell Biology, 2000
It has long been known that microtubule depletion causes axons to retract in a microfilament-dependent manner, although it was not known whether these effects are the result of motor-generated forces on these cytoskeletal elements. Here we show that inhibition of the motor activity of cytoplasmic dynein causes the axon to retract in the presence of ...
F J, Ahmad, J, Hughey, T, Wittmann, A, Hyman, M, Greaser, P W, Baas   +5 more
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Antibodies against vertebrate microfilament proteins in the analysis of cellular motility and adhesion

Journal of Cell Science, 1991
ABSTRACT Microinjection of specific antibodies can be an alternative and a supplement to genetic engineering in dissecting the function of individual cytoskeletal components. In this report, we describe some of the requirements for using this technique, its potential application in conjunction with morphological and biochemical analyses,
B M, Jockusch, B, Zurek, R, Zahn, A, Westmeyer, A, Füchtbauer   +4 more
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Staurosporine induces dissolution of microfilament bundles by a protein kinase C-independent pathway

Experimental Cell Research, 1990
The protein kinase C (PKC) inhibitor staurosporine was found to dramatically alter the actin microfilament cytoskeleton of a variety of cultured cells, including PTK2 epithelial cells, Swiss 3T3 fibroblasts, and human foreskin fibroblasts. For example, PTK2 cells exposed to 20 nM staurosporine exhibited a progressive thinning and loss of cytoplasmic ...
K K, Hedberg, G B, Birrell, D L, Habliston, O H, Griffith   +3 more
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Specific antibody against a protein (p27) present in nonestablished fibroblasts. A putative microfilament-associated protein

Experimental Cell Research, 1987
A specific polyclonal antibody has been raised against a basic cytoplasmic protein (p27) which is induced by serum in growth-arrested NIH 3T3 cells but is constitutively expressed in nonestablished fibroblasts. Immunoblotting analysis and [35S]methionine labeling show that p27 is absent in tissues and established cell lines of different types. However,
J F, Santarén, H, Blüthmann, H, MacDonald-Bravo, R, Bravo   +3 more
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Structural Compartments within Neurons: Developmentally Regulated Organization of Microfilament Isoform mRNA and Protein

Molecular and Cellular Neuroscience, 1998
The microfilament system is thought to be a crucial cytoskeletal component regulating development and mature function of neurons. The intracellular distribution of the microfilament isoform components, actin and tropomyosin (Tm), in neurons primarily in vivo, has been investigated at both the mRNA and the protein level using isoform specific riboprobes
A J, Hannan, P, Gunning, P L, Jeffrey, R P, Weinberger   +3 more
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Early changes in the distribution and organization of microfilament proteins during cell transformation

Cell, 1981
During the onset of transformation, Rous sarcoma virus-infected cells undergo characteristic morphological changes that reflect the biochemical events induced by the viral src gene. Temperature downshift experiments using chick embryo cells infected with transformation-defective temperature-sensitive viral mutants have shown two major morphological ...
C B, Boschek, B M, Jockusch, R R, Friis, R, Back, E, Grundmann, H, Bauer   +5 more
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Protein kinase C affects microfilaments, bone resorption, and [Ca2+]o sensing in cultured osteoclasts

American Journal of Physiology-Cell Physiology, 1992
The effects of protein kinase C (PKC) in the control of osteoclast activity are still unknown. We investigated the role of the enzyme in the control of microfilament organization, podosome assembly, bone resorption, and extracellular Ca2+ sensing in chicken and rabbit osteoclasts treated with agents known to affect PKC activity. Cells were treated for
TETI, ANNA MARIA, COLUCCI S, GRANO M, ARGENTINO L, ZALLONE AZ   +4 more
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