Results 241 to 250 of about 5,523,474 (277)
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Journal of Supramolecular Structure, 1976
AbstractThe localization and organization of actin‐like microfilaments in normal, SV‐40 and adenovirus transformed cells are determined by the coordinated use of light optical, electron optical and biochemical techniques. In adenovirus‐type 5 transformed hamster embryo cells, microfilament meshworks appear to be the predominant organizational form of ...
Marie-Jeanne Yerna +2 more
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AbstractThe localization and organization of actin‐like microfilaments in normal, SV‐40 and adenovirus transformed cells are determined by the coordinated use of light optical, electron optical and biochemical techniques. In adenovirus‐type 5 transformed hamster embryo cells, microfilament meshworks appear to be the predominant organizational form of ...
Marie-Jeanne Yerna +2 more
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Latrunculins: novel marine toxins that disrupt microfilament organization in cultured cells.
Science, 1983Two toxins, latrunculins A and B, which contain a new class of 16- and 14-membered marine macrolides attached to the rare 2-thiazolidinone moiety, were purified recently from the Red Sea sponge Latrunculia magnifica.
I. Spector +3 more
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Angewandte Chemie International Edition, 2018
AbstractProtein allostery, a chemical‐to‐mechanical effect that can precisely regulate protein structure, exists in many proteins. Herein, we demonstrate that protein allostery can be used to drive self‐assembly for the construction of tunable protein architectures. Calmodulin (CaM) was chosen as a model allosteric protein. Ca2+‐mediated contraction of
Miaomiao Xu, Lianxiao Liu, Qiang Yan
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AbstractProtein allostery, a chemical‐to‐mechanical effect that can precisely regulate protein structure, exists in many proteins. Herein, we demonstrate that protein allostery can be used to drive self‐assembly for the construction of tunable protein architectures. Calmodulin (CaM) was chosen as a model allosteric protein. Ca2+‐mediated contraction of
Miaomiao Xu, Lianxiao Liu, Qiang Yan
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Staurosporine induces dissolution of microfilament bundles by a protein kinase C-independent pathway
Experimental Cell Research, 1990The protein kinase C (PKC) inhibitor staurosporine was found to dramatically alter the actin microfilament cytoskeleton of a variety of cultured cells, including PTK2 epithelial cells, Swiss 3T3 fibroblasts, and human foreskin fibroblasts. For example, PTK2 cells exposed to 20 nM staurosporine exhibited a progressive thinning and loss of cytoplasmic ...
K K Hedberg +3 more
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Cortical microfilament proteins and the dynamics of the plasma membrane.
Acta histochemica. Supplementband, 1992The microfilament system is thought to provide motor elements needed for plasma membrane dynamics. This article focuses on two protein components that may play key roles in this process: (1) Profilin, a G-actin binding protein which is considered as the source of actin subunits necessary for rapid changes in the amount of actin filaments.
F, Buss, C, Temm-Grove, B M, Jockusch
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Experimental Cell Research, 1985
Velocity sedimentation by sucrose density gradient centrifugation has been used to characterize ascites microvillar microfilament cores and to identify microfilament-associated proteins. Fluoride, calcium, phalloidin and chemical cross-linking treatments of microvilli during Triton X-100 extractions increase the sedimentation rate of the microfilament ...
Michael L Weiss +1 more
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Velocity sedimentation by sucrose density gradient centrifugation has been used to characterize ascites microvillar microfilament cores and to identify microfilament-associated proteins. Fluoride, calcium, phalloidin and chemical cross-linking treatments of microvilli during Triton X-100 extractions increase the sedimentation rate of the microfilament ...
Michael L Weiss +1 more
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Biochemical Society Transactions, 1991
Fulton, A. €3.. Wan, K. M. & I’enman. S. (10x0) Cell (Cambridge. Mass.) 20, 840-X.57 I m k . K.. Kansom, I,., Kaufmann. Y. 81 I’enman. S. (1077) Cell (Cambridge, Mass.) 10,67-7X Cervera, M., Llreyfuss, G. 81 I’enman, S. (10x1) Cell (Cambridge, Mass.) 23, 1 13120 Zumbe. A., Stahli. C. 81 Trachsel, 11. (10x2) I’roc. Natl. Acad. Sci. lI.S.A. 79.
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Fulton, A. €3.. Wan, K. M. & I’enman. S. (10x0) Cell (Cambridge. Mass.) 20, 840-X.57 I m k . K.. Kansom, I,., Kaufmann. Y. 81 I’enman. S. (1077) Cell (Cambridge, Mass.) 10,67-7X Cervera, M., Llreyfuss, G. 81 I’enman, S. (10x1) Cell (Cambridge, Mass.) 23, 1 13120 Zumbe. A., Stahli. C. 81 Trachsel, 11. (10x2) I’roc. Natl. Acad. Sci. lI.S.A. 79.
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Experimental Cell Research, 1987
A specific polyclonal antibody has been raised against a basic cytoplasmic protein (p27) which is induced by serum in growth-arrested NIH 3T3 cells but is constitutively expressed in nonestablished fibroblasts. Immunoblotting analysis and [35S]methionine labeling show that p27 is absent in tissues and established cell lines of different types. However,
Horst Blüthmann +3 more
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A specific polyclonal antibody has been raised against a basic cytoplasmic protein (p27) which is induced by serum in growth-arrested NIH 3T3 cells but is constitutively expressed in nonestablished fibroblasts. Immunoblotting analysis and [35S]methionine labeling show that p27 is absent in tissues and established cell lines of different types. However,
Horst Blüthmann +3 more
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Journal of Experimental Zoology, 1985
AbstractElectron microscopy and indirect immunofluorescence were employed to correlate the distribution patterns of major contractile proteins (actin and myosin) with 1) the organizational state of microfilaments, 2) the apical cell surface topography, 3) the shape of the neuroepithelial cells, and 4) the degree of bending of the neuroepithelium during
Robert G. Nagele, Hsin-Yi Lee
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AbstractElectron microscopy and indirect immunofluorescence were employed to correlate the distribution patterns of major contractile proteins (actin and myosin) with 1) the organizational state of microfilaments, 2) the apical cell surface topography, 3) the shape of the neuroepithelial cells, and 4) the degree of bending of the neuroepithelium during
Robert G. Nagele, Hsin-Yi Lee
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Microfilaments and protein synthesis; effects of insulin
International Journal of Biochemistry, 1993Anni Vedeler +2 more
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