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Blastocyst Microinjection Automation
IEEE Transactions on Information Technology in Biomedicine, 2009Blastocyst microinjections are routinely involved in the process of creating genetically modified mice for biomedical research, but their efficiency is highly dependent on the skills of the operators. As a consequence, much time and resources are required for training microinjection personnel.
Leonardo S. Mattos +3 more
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Cloning and Stem Cells, 2001
Last year marked the 20th anniversary of the invention of the term "transgenic" and the development of pronuclear microinjection, a straightforward technique designed to transfer genetic information from nearly any living organism to mammals. After two decades of use, pronuclear microinjection protocols have changed little from the reliable, if not ...
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Last year marked the 20th anniversary of the invention of the term "transgenic" and the development of pronuclear microinjection, a straightforward technique designed to transfer genetic information from nearly any living organism to mammals. After two decades of use, pronuclear microinjection protocols have changed little from the reliable, if not ...
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Microinjecting Cells Using a Pulsed-Flow Microinjection System
Cold Spring Harbor Protocols, 2011INTRODUCTIONThe efficient delivery of exogenous DNA to cells for expression and function studies is an essential technique of modern cell biology, and direct delivery of genetic material by microinjection remains a reliable means of transfection. A pulsed-flow microinjection system, such as the Eppendorf InjectMan and FemtoJet systems, permits a high ...
David A, Dean, Joshua Z, Gasiorowski
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Mosquito Embryo Microinjection
Cold Spring Harbor Protocols, 2023Genetically modified (GM) mosquitoes are an important tool in the fight against mosquito-borne disease, both indirectly through their use in research investigating host–pathogen interaction, mosquito olfaction, and anthropomorphic behavior and in future direct uses for suppression and possibly eradication through sterile insect technique (SIT) and/or ...
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Microinjection Methods for Neutrophils
2014The ability to microinject substances into the cytosol of living neutrophils opens the possibility of manipulating the chemistry within the cell and also of monitoring changes using indicators which otherwise cannot be introduced into the cell. However, neutrophils cannot be microinjected by the conventional glass pipette insertion method.
Iraj, Laffafian +3 more
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Generation of Chimeras by Microinjection
2009Since the technique of introducing a targeted mutation ('gene targeting') into the mouse genome was published almost 20 years ago (Cell 51:503-512, 1987), the number of mouse mutants (mouse models) is increasing, especially after the advent of the full mouse genomic sequence in 2002 and the human genomic sequences in 2003 that reveals more and more ...
Anne, Plück, Christian, Klasen
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Microinjection of Protein Samples
Cold Spring Harbor Protocols, 2007INTRODUCTIONThis protocol describes a method for microinjecting proteins into the nucleus or cytoplasm of adherent cells. Microinjection equipment can be obtained from a number of suppliers; this protocol has been used with the Narishige IM-200 air pressure regulator and the Leitz micromanipulator.
Yulia, Komarova +2 more
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