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2001
Abstract During recent decades, microinjection has developed into an important method used by cell biologists, and there have been several thousand papers published describing applications of this technique. The main advantage of microinjection is that it enables the direct introduction of substances into individual cells or ...
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Abstract During recent decades, microinjection has developed into an important method used by cell biologists, and there have been several thousand papers published describing applications of this technique. The main advantage of microinjection is that it enables the direct introduction of substances into individual cells or ...
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Plating Cells for Microinjection
Cold Spring Harbor Protocols, 2006INTRODUCTIONThis protocol describes a method for plating cells for microinjection onto etched coverslips. The coverslips for microinjection must be marked so that microinjected cells can be identified at time points after injection. A procedure for etching coverslips is given below; alternatively, pre-etched coverslips can be purchased at slightly ...
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Science, 1966
A technique has been developed for injecting known amounts of liquids into fertilized one-celled mouse eggs by use of a calibrated ocular micrometer superimposed on the terminal regions of the injection pipette. From 128 pronuclear eggs, each injected with 180 or 770 cubic microns of bovine gamma globulin at a concentration of 25 milligrams per ...
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A technique has been developed for injecting known amounts of liquids into fertilized one-celled mouse eggs by use of a calibrated ocular micrometer superimposed on the terminal regions of the injection pipette. From 128 pronuclear eggs, each injected with 180 or 770 cubic microns of bovine gamma globulin at a concentration of 25 milligrams per ...
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Implantation of Microinjected Eggs
2003Microinjected eggs are normally incubated overnight in Ml6 microdrop culture at 37°C in CO₂ until 1400 h. By this time the eggs will have developed as far as the two-cell stage. The eggs are then implanted into surrogate mothers. As the membrane covering the oviduct of the rat is much tougher than the mouse, we normally cut it with a very fine pair of ...
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