Results 71 to 80 of about 17,415 (301)

Microinjection of mRNAs and Oligonucleotides [PDF]

Cold Spring Harbor Protocols, 2018
Microinjecting lineage tracers into a single blastomere in the normal, intact embryo identifies the repertoire of cell types derived from it. In order to reveal the full developmental potential of that blastomere or identify the mechanisms by which its fate is determined, one needs to modify its gene expression under controlled experimental conditions.
openaire   +2 more sources

Anatomical sites of lateral septal area microinjections.

, 2013
A photomicrograph of a coronal brain section showing bilateral microinjections sites in the lateral septal area (LSA) and a diagrammatic representation based on the rat brain atlas of Paxinos and Watson (1997) indicating injections sites of vehicle (open
Leonardo B. M. Resstel (346236), Francisco S. Guimarães (346234), América A. Scopinho (346233), Fernando M. A. Corrêa (346235), Daniel G. Reis (266121)   +4 more
core   +1 more source

Paternal Circadian Disruption Impairs Offspring Cognition via Sperm microRNAs

Advanced Science, EarlyView.
Paternal circadian disruption remodels the sperm small RNA payload, elevating miR‐92a‐3p/miR‐25‐3p levels and perturbing early embryonic gene regulatory programs. Microinjection experiments and single‐embryo transcriptomics reveal sex‐specific developmental vulnerabilities, ultimately impairing offspring hippocampal synaptic plasticity and cognition ...
Kexin Zou, Sisi Luo, Binliang Tang, Zhiqiang Liu, Yuchuan Zhou, Yicong Meng, Zhengmu Wu, Zheng Sun, Weihui Shi, Jianzhong Sheng, Chuanjin Yu, Zhenhua Li, Siyi Wei, Mo Zhang, Yujie Luo, Jing Yan, Xueyun Qin, Jiahang Mo, Chengliang Zhou, Jinsong Li, Hefeng Huang, Yifu Ding, Guolian Ding   +22 more
wiley   +1 more source

Targeting the PDK1/c‐Myc/SOX10 Signaling in Oligodendrocytes Alleviates Neuropathic Pain

Advanced Science, EarlyView.
This work reveals that oligodendrocyte homeostasis, mediated by PDK1, is a critical determinant of neuropathic pain (NPP) pathogenesis. Disruption of PDK1 in oligodendrocytes impairs SOX10‐dependent myelination programs through c‐Myc accumulation, leading to disrupted myelination and the pathophysiology of NPP.
Pingping Qiao, Lifang Guo, Guochao Yang, Chaoli Huang, He Wang, Jian‐Jun Yang, Guiquan Chen, Yimin Hu   +7 more
wiley   +1 more source

Receptor subtypes mediating depressor responses to microinjections of nicotine into medial NTS of the rat

, 2000
Microinjections (50 nl) of nicotine (0.01–10 μM) into the nucleus of the solitary tract (NTS) of adult, urethan-anesthetized, artificially ventilated, male Wistar rats, elicited decreases in blood pressure and heart rate.
J. M. McIntosh, F. Nagy, H. N. Sapru, S. Dhar   +3 more
core   +1 more source

Circular RNA circNrip1 Interacts with SYNCRIP to Promote Neuropathic Pain by Stabilizing Tlr2 mRNA in Primary Sensory Neurons

Advanced Science, EarlyView.
Model of circNrip1 (cNrip1) upregulation driving neuropathic pain mechanisms. After peripheral nerve injury, increased FUS triggers the formation and upregulation of cNrip1 in injured DRG neurons. Upregulated cNrip1 recruits SYNCRIP to the 3′‐UTR of Tlr2 mRNA by binding to both, thereby promoting SYNCRIP‐triggered Tlr2 mRNA stability and increasing ...
Xiaozhou Feng, Jifang Xiao, Congcong Huang, Bao‐Chun Jiang, Bing Wang, Xu Li, Shibin Du, Tolga Berkman, Chun‐Hsien Wen, Weihua Cai, Dilip Sharma, Kun Wang, Longfei Ma, Huijuan Hu, Yuan‐Xiang Tao   +14 more
wiley   +1 more source

A Hypothalamic Leptin-Glutamate Interaction in the Regulation of Sympathetic Nerve Activity

Neural Plasticity, 2017
Accumulated evidence indicates that obesity-induced type 2 diabetes (T2D) is associated with enhanced sympathetic activation. The present study was conducted to investigate the role for leptin-glutamate signaling within the hypothalamus in regulating ...
Hong Zheng, Xuefei Liu, Yulong Li, Kaushik P. Patel   +3 more
doaj   +1 more source

The effect of microinjection of dimethyl sulfoxide into the rostral ventromedial medulla on swim stress-induced analgesia [PDF]

The Journal of Qazvin University of Medical Sciences, 2018
Background: Dimethyl sulfoxide (DMSO) is an important solvent for compounds that used in pain research. Rostral ventromedial medulla (RVM) plays an important role in modulating nociception and stress-induced analgesia (SIA).
S. Nazemi, A. Shamsizadeh, N. Haidari-Oranji, N. Soleimani, H. Azhdari Zarmehri   +4 more
doaj   +1 more source

Statistical Study of the Energetic Electron Microinjections at the High-latitude Magnetosphere

, 2022
Understanding the formation of the seed population for the energetic electrons trapped within the Earth’s Van Allen radiation belts has been under debate for decades. The magnetic reconnection in the Earth’s magnetotail during the substorms is considered
Nykyri, Katariina, Liou, Yu-Lun, Ma, Xuanye, Kavosi, Shiva   +3 more
core  

Nigra‐Subthalamic Dopaminergic Circuitry Modulates and Represents Distinct Pain Modality in Physiological and Pain States in Mice

Advanced Science, EarlyView.
Nigral dopaminergic (DA) neurons modulate and represent pain with a preference to a particular modality (mechanical) and laterality (contralateral), which are controlled by nigral GABAergic neurons. The pain modulation is mimicked by the nigro‐subthalamic projection and its downstream neurons, involving D2‐like receptors.
Ying Ji, Shuyi Li, Jiaqi Zhang, Xiang‐Ying Xu, Cui Yin, Mu Niu, Danyang Liu, Yanyu Jiang, Yong Niu, Su Liu, Bin Zhang, Guiyun Cui, Chunyi Zhou, Cheng Xiao   +13 more
wiley   +1 more source