Results 71 to 80 of about 18,274 (287)

Compact 9dBEs Enable Efficient and Precise Genome Editing in Mammalian Cells and In Vivo

Advanced Science, EarlyView.
As a compact type II‐D system, the Cas9d‐based platform holds great potential for in vivo applications. Through rational engineering, its derived base editors (9dBEs) enable efficient disease modeling while facilitating single‐vector AAV delivery for in vivo genome editing. These miniature tools offer a robust strategy for basic research and biomedical
Qingquan Xiao, Zhijin Tian, Luqi Weng, Kun Xu, Dingyi Han, Zhaowei Wu, Junnan Li, Shangpu Li, Qijing Zhang, Xiaohui Zhao, Guoling Li, Yinghui Wei, Zhen Gu   +12 more
wiley   +1 more source

Neural Stem Cells Transplantation as a Neuroprotective Strategy for Amyotrophic Lateral Sclerosis (ALS)

Global Spine Journal, 2016
Introduction Amiotrophic lateral sclerosis (ALS) is a neurodegenerative disease with a prevalence rate of up to 7.4/100,000 and the overall risk of developing ALS over a lifetime is 1:400.
Sandro Carletti, Rossella Rispoli, Alessandro Di Chirico, Cristina Spera, Lorenzina Bolli, Maria Laura Scarcella, Domenica Frondizzi, Giuseppe Stipa, Letizia Mazzini, Maurizio Gelati, Angelo Vescovi   +10 more
doaj   +1 more source

Increased and Decreased Muscle Tone With Orexin (Hypocretin) Microinjections in the Locus Coeruleus and Pontine Inhibitory Area

, 2001
Orexin-A (OX-A) and orexin-B (OX-B) (hypocretin 1 and hypocretin 2) are synthesized in neurons of the perifornical, dorsomedial, lateral, and posterior hypothalamus.
Lyudmila I. Kiyashchenko, Jerome M. Siegel, Yuan-Y. Lai, Boris Y. Mileykovskiy   +3 more
core   +1 more source

Gut Microbiota‐Isoallolithocholic Acid Crosstalk Promotes Calcium Oxalate Kidney Stone Formation via PARP1‐Mediated Parthanatos

Advanced Science, EarlyView.
ABSTRACT Bile acids have been implicated in calcium oxalate (CaOx) nephrolithiasis. Here, we employ multi‐omics approaches to identify isoallolithocholic acid (isoalloLCA) as the key bile acid elevated in the feces, serum, kidney, and urine of CaOx rats, confirmed by spatial metabolomics.
Zijian Zhou, Ruixi Yu, Xuan Zhou, Feng Shi, Zeyu Lin, Peng Gao, Lujia Wang, Ninghan Feng, Zhong Wu, Dawei Wang   +9 more
wiley   +1 more source

Hydrogen sulphide in the RVLM and PVN has no effect on cardiovascular regulation

Frontiers in Physiology, 2011
Hydrogen Sulphide (H2S) is now recognised as an important signalling molecule and has been shown to have vasodilator and cardio-protectant effects. More recently it has been suggested that H2S may also act within the brain to reduce blood pressure.
Eloise eStreeter, Mohammad eAl-Magableh, Joanne Louise Hart, Emilio eBadoer   +3 more
doaj   +1 more source

REM sleep-like episodes of motoneuronal depression and respiratory rate increase are triggered by pontine carbachol microinjections inin situperfused rat brainstem preparation

, 2011
Hypoglossal nerve activity (HNA) controls the position and movements of the tongue. In persons with compromised upper airway anatomy, sleep-related hypotonia of the tongue and other pharyngeal muscles causes increased upper airway resistance, or total ...
Kubin, Leszek, Georg M. Stettner, Brandes, Ivo Florian, Mathias Dutschmann, Moerschel, Michael, Leszek Kubin, Dutschmann, Mathias, Michael Mörschel, Stettner, Georg M., Ivo F. Brandes   +9 more
core   +1 more source

The Adipose‐Sympathetic Nerve Crosstalk: FSTL1 as an Adipocyte‐Derived Neurotrophic Factor for WAT Browning

Advanced Science, EarlyView.
As a novel adipose‐derived neurotrophic factor, Follistatin‐like 1 is endocytosed by sympathetic neurons primarily via tropomyosin‐related kinase B. This process promotes sympathetic innervation in adipose tissue and norepinephrine release, leading to white adipose tissue browning, enhanced thermogenesis, and anti‐obesity effects in mice.
Xiao‐Wei Jia, Jia‐Xin Liu, Rui‐Ping Wang, Xue‐Qin Wu, Yao Che, Mu‐Chen Wu, Hong‐Wei Shang, Xin Lu, Tao Lu, Lu‐Lu Wang, Chun‐Yan Jiang, Dong‐Liang Fang, Chun Yang, Yan Gao   +13 more
wiley   +1 more source

Microinjections observed by MMS FEEPS in the dusk to midnight region

Geophysical Research Letters, 2016
Energetic electron injections are commonly observed in the premidnight to dawn regions in association with substorms. However, successive electron injections are generally separated in time by hours and are rarer in the dusk region of the inner ...
J. F. Fennell, D. L. Turner, C. L. Lemon, J. B. Blake, J. H. Clemmons, B. H. Mauk, A. N. Jaynes, I. J. Cohen, J. H. Westlake, D. N. Baker, J. V. Craft, H. E. Spence, G. D. Reeves, R. B. Torbert, J. L. Burch, B. L. Giles, W. R. Paterson, R. J. Strangeway   +17 more
doaj   +1 more source

Expression of opioid receptors and dre-let-7d after opioid receptor DNA microinjections.

, 2013
(A) Quantification by qPCR of zfmor, zfdor1 and zfdor2 receptors at 48 hpf (after opioid receptor DNA microinjections). Expression of dre-let-7d-1 (B, D, F and H), dre-let-7d-2 (C, E, G and I) and dre-let-7d (J) after opioid receptor DNA microinjections:
Raquel E. Rodríguez (109036), Roger López-Bellido (109032), Fátima Macho Sánchez-Simón (109034), Katherine Barreto-Valer (109031)   +3 more
core   +1 more source

Phase Separation of SF3B1 Serves as a Critical Post‐Transcriptional Regulator During Early Mouse Embryogenesis

Advanced Science, EarlyView.
Phase separation of SF3B1 acts as a key regulatory mechanism for dynamic alternative splicing throughout early mouse embryogenesis. Its absence triggers extensive splicing errors, which induce persistent DNA damage, defective cell cycle progression, and failed cell lineage commitment, and ultimately hinder the normal growth and development of ...
Kang Zhao, Ting‐Yu Han, Yan‐Li Cheng, Yi‐Dan Zhang, Yu‐Wei Zhang, JinJian Guo, ShaoJun Zhang, WenZe Huang, Jing Zhang, Pei‐Yu Liao, Ying Xin, ChuanChen Chu, Qing‐Yuan Sun, ZhiZhen Liu, Xiang‐Hong Ou, Jun Xie   +15 more
wiley   +1 more source