Results 81 to 90 of about 15,619 (228)

Establishment of Humanized EFNB2/B3 Murine Models for Investigating Nipah Virus Pathogenesis

Animal Research and One Health, EarlyView.
ABSTRACT Nipah virus (NiV) is a zoonotic paramyxovirus classified as a WHO priority pathogen, which causes severe respiratory disease and encephalitis in humans with high mortality. Currently, no licensed vaccines or specific antiviral therapies are available.
Wei Dong, Ning Liu, Weining Kong, Xiang Gao, Feifei Guan, Shan Gao, Yuanwu Ma   +6 more
wiley   +1 more source

Localization of RNA transcription sites in insect oocytes using microinjections of 5-bromouridine 5'-triphosphate.

Folia Histochemica et Cytobiologica, 2007
In the present study we used 5-bromouridine 5'-triphosphate (BrUTP) microinjections to localize the transcription sites in oocytes of insects with different types of the ovarium structure: panoistic, meroistic polytrophic, and meroistic telotrophic.
Dmitry Bogolyubov
doaj   +1 more source

Metabolic fate of drugs of abuse and new psychoactive substances: A pilot study on a novel workflow using a zebrafish embryo model combined with human microdosing

British Journal of Clinical Pharmacology, EarlyView.
Aim The aim of this study was to develop a novel workflow to identify human urine biomarkers for drugs of abuse and new psychoactive substances. Metabolites of amphetamine, cocaine, LSD, MDMA, methamphetamine, THC, MDMB‐CHMICA, and MDPPP were first identified in a zebrafish embryo (ZE) metabolism study followed by comparison to most abundant human ...
Simon K. Wellenberg, Tanja M. Gampfer, Lea Wagmann, Philip Schippers, Jennifer Herrmann, Rolf Müller, Folker Westphal, Markus R. Meyer   +7 more
wiley   +1 more source

Status and future of recombinant adeno‐associated virus vector manufacturing

Biotechnology Progress, EarlyView.
Abstract Sixty years of adeno‐associated virus (AAV) research illustrates a trajectory marked by basic science exploration, iterative innovation, persistent challenges, a number of clinical setbacks, as well as commercial therapeutic triumphs. This continual evolution has led to recombinant AAV (rAAV) becoming a cornerstone of modern gene therapy ...
Frank Agbogbo, David Dismuke
wiley   +1 more source

Embryonic development of the Mediterranean starfish Hacelia attenuata

Developmental Dynamics, EarlyView.
Abstract Background Starfish play essential ecological roles as predators and ecosystem regulators; however, detailed developmental descriptions exist for only a handful of species, none of which are from the Mediterranean Sea. Results In this study, we provide the first full account of the development of the Mediterranean starfish Hacelia attenuata ...
Silvia Caballero‐Mancebo, Laurent Gilletta, Janet Chenevert, Stefania Castagnetti   +3 more
wiley   +1 more source

Protocol for microinjection of rapamycin into the zebrafish habenula

STAR Protocols
Summary: Mechanistic target of rapamycin complex 1 (mTorC1) activity plays a crucial role in brain development. Here, we present an approach for rapamycin microinjection into the habenula of larval zebrafish to achieve localized inhibition of the mTorC1 ...
Olga Doszyn, Tomasz Dulski, Justyna Zmorzynska   +2 more
doaj   +1 more source

Zebrafish ventral gastrula fate map reveals neural crest progenitor domain

Developmental Dynamics, EarlyView.
Abstract Background Fate maps relate progenitor cell positions to later fates and locations of their progeny, revealing early embryonic organization. Previous zebrafish fate maps identified the origin of germ layers and derivative cell fates, but ventral gastrula progenitor domains were not fully resolved. In particular, the neural crest, a multipotent
Elaine E. Kushkowski, Victoria E. Prince
wiley   +1 more source

Unique deficits in place coding across subfields of the hippocampus in a mouse model of temporal lobe epilepsy

Epilepsia, EarlyView.
Abstract Objective Memory problems are comorbid with temporal lobe epilepsy (TLE). Animal models of TLE reveal impairments in spatial firing fields of hippocampal place cells, providing a potential neural substrate for memory problems. Each subfield of the hippocampus carries out unique aspects of spatial memory, yet little is known about how ...
Brittney L. Boublil, Margaret M. Donahue, Cathy B. Dang, Gergely Tarcsay, Laura A. Ewell   +4 more
wiley   +1 more source

Epilepsy surgery: From bench to the clinics

Epilepsia Open, EarlyView.
Abstract Objective Recent advances in epilepsy surgery in patients with intractable epilepsy make it possible to study the mechanism of epilepsy in human brains. However, the true extent and propagation of each epileptogenic area from the epileptogenic focus in each patient is still difficult to perform “epilepsy cure” by surgery.
Tatsuya Tanaka
wiley   +1 more source

Arterial Perivascular Space‐Mediated Solute Transport in the Mouse Brain

Exploration, EarlyView.
The mechanisms underlying solute clearance from the brain parenchyma remain debated, with competing hypotheses involving bulk cerebrospinal fluid flow versus perivascular transport. Using multimodal in vivo imaging and computational modeling in mice, this study demonstrates that arterial pulsation drives bidirectional solute movement within the ...
Shiyong Li, Ye Wang, Guangyu Jia, Yamei Yu, Zhe Zhang, Qi Wang, Kaelyn V. Becker, Jonathan W. Engle, Dawei Jiang, Weibo Cai   +9 more
wiley   +1 more source