Results 101 to 110 of about 23,889 (290)

Suitability of a CMV/EGFP cassette to monitor stable expression from human artificial chromosomes but not transient transfer in the cells forming viable clones [PDF]

, 2004
Human artificial chromosomes (HACs) were generated by transfer of telomerized PAC constructs containing alpha satellite DNA of various human chromosomes. To monitor which cells took up constructs and subsequently formed stable clones under blasticidin S (
Christan, S., Laner, A., Schindelhauer, D., Schwarz, T.   +3 more
core   +1 more source

Plant Genetic Engineering: Technological Pathways, Application Scenarios, and Future Directions

Advanced Science, EarlyView.
This review maps the fast‐evolving landscape of plant genetic engineering, linking enabling platforms with trait‐focused applications in architecture optimization, stress resilience, yield improvement, and quality enhancement. It highlights how genome editing, transgenic strategies, and emerging multi‐gene approaches reshape breeding pipelines, while ...
Peilin Wang, Wenzhi Wang, Dongjiao Wang, Qibin Wu, Youxiong Que   +4 more
wiley   +1 more source

Interaction of hypothalamic GABA\u3csub\u3eA\u3c/sub\u3e and excitatory amino acid receptors controlling heart rate in rats [PDF]

, 1991
We have previously shown that microinjection of drugs that impair gamma-aminobutyric acid (GABA)-mediated synaptic inhibition into the dorsomedial hypothalamus (DMH) of rats generates cardiovascular and behavioral changes that mimic the response to ...
DiMicco, Joseph A., Soltis, Robert P.
core   +2 more sources

Transformation and microinjection [PDF]

WormBook, 2006
Copyright: © 2006 Thomas C. Evans and contributors. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and contributors reproduction in any medium, provided the original author and source are credited. To whom correspondence should be addressed.
openaire   +1 more source

An Activity‐Dependent NEPAS–PTX3 Axis Links Neurovascular and Myelin Deficits to Cognitive Impairment

Advanced Science, EarlyView.
An activity‐dependent pathway links prefrontal circuit hypoactivity to cognitive impairment. Reduced PVA–mPFC activity upregulates NEPAS, which suppresses PTX3 secretion, leading to impaired angiogenesis, myelin deficits, and memory decline. Rescue is achieved by NEPAS knockdown or chemogenetic circuit activation.
Boya Hu, Zifei Chen, Bingmei Sun, Jiale Gao, Jiale Xu, Xiaochun Guo, Fenfei Gao, Zhongsi Wang, Jie Wu, Xiaoyu Ji, Peipei Liu, Bing Huang   +11 more
wiley   +1 more source

TULP4, a novel E3 ligase gene, participates in neuronal migration as a candidate in schizophrenia

CNS Neuroscience &Therapeutics, EarlyView., 2023
Mutations identified from four SCZ pedigrees resulted in decreased TULP4 expression. Tulp4 knockdown caused delayed neuron migration in embryonic mice, and impaired cognition and prepulse inhibition in adult mice. These phenotypes may be related to TULP4 through its involvement in the formation of a novel E3 ubiquitin ligases.
Yan Bi, Decheng Ren, Fan Yuan, Zhou Zhang, Daizhan Zhou, Xin Yi, Lei Ji, Keyi Li, Fengping Yang, Xi Wu, Xingwang Li, Yifeng Xu, Yun Liu, Peng Wang, Changqun Cai, Chuanxin Liu, Qian Ma, Lin He, Yi Shi, Guang He   +19 more
wiley   +1 more source

Role of the thalamic parafascicular nucleus cholinergic system in the modulation of acute corneal nociception in rats [PDF]

, 2011
The present study investigated the effects of microinjections of acetylcholine (a cholinergic agonist), physostigmine (a cholinesterase inhibitor), atropine (an antagonist of muscarinic cholinergic receptors) and hexamethonium (an antagonist of nicotinic
Amir-Abbas Farshid, Asghar Morvaridi, Esmaeal Tamaddonfard, Sona Seiednejhad   +3 more
core  

Temporal properties of cerebellar-dependent memory consolidation [PDF]

, 2004
Classical conditioning of the nictitating membrane response in rabbits is a well defined model of cerebellar-dependent motor memory. This memory undergoes a period of consolidation after the training session, when it is sensitive to reversible ...
Attwell, PJE, Cooke, SF, Yeo, CH
core   +1 more source

CTBPro: A Next‐Generation Cholera Toxin Subunit B‐Based Neuroanatomical Tracer With Superior Brightness, Stability, and Sensitivity for Enhanced Neural Circuit Mapping

Advanced Science, EarlyView.
CTBPro is a next‐generation cholera toxin B–based tracer engineered by fusing CTB to the ultra‐stable fluorescent protein mBaojin. Exhibiting markedly enhanced molar brightness, CTBPro enables high‐fidelity neuronal labeling across multiple administration routes.
Xinghua Quan, Yude Lou, Huiqi Xie, Yifei Wang, Yanzhe Zhang, Yiran Ge, Linhe Yang, Xiaoxuan Zhang, Qingmiao Zhou, Huaizong Shen, Longxing Cao, Xin Jin, Jie‐Min Jia   +12 more
wiley   +1 more source

Interaction between estradiol and 5-HT1A receptors in the median raphe nucleus on acquisition of aversive information and association to the context in ovariectomized rats

Neurobiology of Stress, 2017
The median raphe nucleus (MRN) is related to stress resistance and defensive responses, a crucial source of serotonergic neurons that project to prosencephalic structures related to stress and anxiety.
Telma Gonçalves Carneiro Spera de Andrade, João Victor dos Santos Silva, Matheus Fitipaldi Batistela, Fernando Frei, Ana Beatriz Sant’Ana   +4 more
doaj   +1 more source