Results 101 to 110 of about 17,415 (301)

Towards the Automation of Embryonic Stem Cell Microinjections into Blastocysts

, 2009
The purpose of the research has been to increase the consistency and efficiency rates of blastocyst microinjections through automation. The research involved the design, implementation, and evaluation of a novel biomanipulation system that is a test-bed
Mattos, Leonardo Serra
core  

Energetic Electron Microinjections Observed by MMS in the Dusk Plasma Sheet and Drift Resonance Interpretation

, 2022
Microinjection phenomena, characterized by dispersive oscillations of electron fluxes at the Pc5 period and bi-directional pitch angle anisotropy, are frequently observed by Magnetospheric Multiscale in the dusk to midnight plasma sheet.
J. L. Burch, R. B. Torbert, P.‐A. Lindqvist, Qiugang Zong, Zuyin Pu, Torbert, R. B.,, Suiyan Fu, Luo, Zhekai,, Burch, J. L.,, Zhou, Xuzhi,, Li Li, Ying Xiong, Xuzhi Zhou, Zong, Qiugang,, Xiong, Ying,, Fu, Suiyan,, Blake, J. B.,, Lun Xie, R. E. Ergun, J. B. Blake, Lindqvist, Per-Arne,, Zhekai Luo, Ergun, R. E.,, Pu, Zuyin,, C. T. Russell, Russell, C. T.,, Xie, Lun,, Li, Li,   +27 more
core   +1 more source

Making Sweat Measurable: Induction, Sampling, and Refreshment in Wearable Biofluid Sensing

Advanced Science, EarlyView.
Wearable sweat sensing relies not only on chemical detection but also on controlled biofluid management. This Review integrates sweat physiology, induction strategies, and microfluidic sampling architectures, demonstrating how flux, transport, and refreshment shape measurement reliability.
Soyoung Shin, Wei Gao
wiley   +1 more source

NAD+ Metabolism Licenses Zygotic Genome Activation via PARP7‐Mediated ADP‐Ribosylation of UHRF1 in Mouse Early Embryos

Advanced Science, EarlyView.
This study uncovers a metabolic‐epigenetic axis licensing zygotic genome activation (ZGA) in mouse embryos. A developmental decline in NAD+ levels activates PARP7, which mono‐ADP‐ribosylates and stabilizes UHRF1. This modification promotes the establishment of permissive histone acetylation marks, thereby facilitating timely ZGA.
Guangyi Cao, Ziqi Zhang, Anqi Chen, Yanbo Liu, Yuling Lin, Lina Yu, Ruixin Shi, Aolei Guo, Yan Mao, Ganggui Lou, Chaojun Li, Luhong Wen, Guijun Yan, Haixiang Sun   +13 more
wiley   +1 more source

Stress-free microinjections in conscious rats

, 2011
Microinjections are a major tool in modern neuroscience. Microinjection techniques in conscious animals typically involve four steps: 1) animal adapts to experimental setup; 2) injection system is filled and the microinjector is carefully inserted; 3) a ...
Maria V. Zaretskaia, Rusyniak, Daniel E., Joseph A. DiMicco, DiMicco, Joseph A., Dmitry V. Zaretsky, Daniel E. Rusyniak, Zaretskaia, Maria V., Zaretsky, Dmitry V.   +7 more
core   +1 more source

MIS12 Is Required for Kinetochore‐Microtubule Attachment in Oocyte Meiosis

Advanced Science, EarlyView.
A model depicting the role of MIS12 in K‐MT attachment during oocyte meiosis. The presence of MIS12 stabilizes bipolar K‐MT attachments by maintaining the function of NDC80 and its interaction with TUBB. This, in turn, promotes KNL1 assembly and subsequent SAC protein recruitment to kinetochores.
Jian Li, Chun‐Hui Zhang, Yong Wang, Cheng‐Yuan Li, Da‐Rong Wen, Xi Xia, Liang Zhou, Wei‐Ping Qian, Qing‐Yuan Sun, Chang‐Zhong Li   +9 more
wiley   +1 more source

Behavioral Effects of Systemic, Infralimbic and Prelimbic Injections of a Serotonin 5-HT2A Antagonist in Carioca High- and Low-Conditioned Freezing Rats

Frontiers in Behavioral Neuroscience, 2017
The role of serotonin (5-hydroxytryptamine [5-HT]) and 5-HT2A receptors in anxiety has been extensively studied, mostly without considering individual differences in trait anxiety.
Laura A. León, Laura A. León, Laura A. León, Vitor Castro-Gomes, Santiago Zárate-Guerrero, Karen Corredor, Antonio P. Mello Cruz, Marcus L. Brandão, Fernando P. Cardenas, J. Landeira-Fernandez   +9 more
doaj   +1 more source

The use of microinjections aginst bark beetles on spruce in forestry praxis

, 2020
The report summarizes the results of toxicity tests of various bioinsecticides and their field applications to spruce trees via microinjections to protect stands against the spruce bark beetle, Ips ...
Doležal, Petr
core  

Expression of opioid receptors and dre-let-7d after opioid receptor DNA microinjections.

, 2013
(A) Quantification by qPCR of zfmor, zfdor1 and zfdor2 receptors at 48 hpf (after opioid receptor DNA microinjections). Expression of dre-let-7d-1 (B, D, F and H), dre-let-7d-2 (C, E, G and I) and dre-let-7d (J) after opioid receptor DNA microinjections:
Raquel E. Rodríguez (109036), Roger López-Bellido (109032), Fátima Macho Sánchez-Simón (109034), Katherine Barreto-Valer (109031)   +3 more
core   +1 more source

AI‐Assisted Digital Single‐Molecule Activity Tracker for Decoupling Intrinsic Heterogeneity from Photo‐Oxidative Damage in High‐Photon‐Flux Enzymology

Advanced Science, EarlyView.
Employing a digital single‐molecule activity tracker (dSMAT), this research demonstrates that high‐photon‐flux irradiation drives progressive oxidative scarring in polymerases. Unlike simple thermal denaturation, real‐time kinetic tracking dynamically visualizes enzymes degrading into multiple impaired subpopulations.
Anran Zheng, Qi Yang, Jinze Li, Fuqiang Ma, Xuefeng Wang, Zhen Guo, Chuanyu Li, Dongshu Li, Jia Yao, Zhiqi Zhang, Wei Zhang, Lianqun Zhou   +11 more
wiley   +1 more source