Results 121 to 130 of about 18,274 (287)

Engineering Biology Beyond Single Genes: Advances and Challenges in Multiplex Genome Editing

Animal Research and One Health, EarlyView.
Multiplex genome editing is transforming genome engineering from single‐gene perturbation to network‐level control, yet its broader application remains limited by challenges in gRNA array engineering, delivery technologies, and safety management. Emerging AI‐driven approaches are accelerating guide RNA design and CRISPR effector optimization for ...
Linli Wang, Yongbin Liu, Hongbing Han
wiley   +1 more source

Metabolic fate of drugs of abuse and new psychoactive substances: A pilot study on a novel workflow using a zebrafish embryo model combined with human microdosing

British Journal of Clinical Pharmacology, EarlyView.
Aim The aim of this study was to develop a novel workflow to identify human urine biomarkers for drugs of abuse and new psychoactive substances. Metabolites of amphetamine, cocaine, LSD, MDMA, methamphetamine, THC, MDMB‐CHMICA, and MDPPP were first identified in a zebrafish embryo (ZE) metabolism study followed by comparison to most abundant human ...
Simon K. Wellenberg, Tanja M. Gampfer, Lea Wagmann, Philip Schippers, Jennifer Herrmann, Rolf Müller, Folker Westphal, Markus R. Meyer   +7 more
wiley   +1 more source

Influence of microinjections of D,L-homocysteic acid into the Bötzinger complex area on the cough reflex in the cat.

, 2008
Microinjections of D,L-homocysteic acid (DLH) were used to test the hypothesis that neuronal activation within the Botzinger complex area can modify the spatiotemporal characteristics of the cough reflex in 17 spontaneously breathing pentobarbitone ...
C Wang, I Poliacek, L W Corrie, M J Rose, D C Bolser   +4 more
core  

Group data showing the effect of barodenervation on ARCN responses.

, 2013
A: Microinjections of NMDA (10 mM, 20 nl) into the ARCN before barodenervation (open bars) elicited decreases in MAP (n = 5). Bilateral barodenervation elicited increases in baseline MAP, GSNA and HR (not shown) which lasted for 60 min. When baseline MAP
Tetsuya Kawabe (292023), Hreday N. Sapru (292027), Kazumi Kawabe (292025)   +2 more
core   +1 more source

Embryonic development of the Mediterranean starfish Hacelia attenuata

Developmental Dynamics, EarlyView.
Abstract Background Starfish play essential ecological roles as predators and ecosystem regulators; however, detailed developmental descriptions exist for only a handful of species, none of which are from the Mediterranean Sea. Results In this study, we provide the first full account of the development of the Mediterranean starfish Hacelia attenuata ...
Silvia Caballero‐Mancebo, Laurent Gilletta, Janet Chenevert, Stefania Castagnetti   +3 more
wiley   +1 more source

Forebrain mechanisms of pain and analgesia : effects of local anaesthetic and NMDA antagonist microinjections on persistent pain

, 1996
This series of experiments examined the neural mechanisms of analgesia caused by local anaesthetic blockade or selective blockade of N-methyl- scD-aspartate (NMDA) receptors at sites in the rat forebrain.
McKenna, John E. (John Erwin)
core  

Zebrafish ventral gastrula fate map reveals neural crest progenitor domain

Developmental Dynamics, EarlyView.
Abstract Background Fate maps relate progenitor cell positions to later fates and locations of their progeny, revealing early embryonic organization. Previous zebrafish fate maps identified the origin of germ layers and derivative cell fates, but ventral gastrula progenitor domains were not fully resolved. In particular, the neural crest, a multipotent
Elaine E. Kushkowski, Victoria E. Prince
wiley   +1 more source

Evidence for functional convergence between LZTS2 and DYRK1A during craniofacial development

Developmental Dynamics, EarlyView.
Abstract Background Precise control of DYRK1A dosage is essential for embryonic development, including craniofacial morphogenesis. While LZTS2 is among the most consistently identified DYRK1A‐interacting proteins, its roles in embryonic development remain incompletely understood, and its potential contribution to craniofacial development has not been ...
Nicole Cheng, Santiago Lima, Larisa Litovchick, Amanda J. G. Dickinson   +3 more
wiley   +1 more source

Protocol to assess the impact of sperm RNA on mouse preimplantation embryos using microinjection

STAR Protocols
Summary: Zygotic microinjections show that sperm RNAs transmit non-genetically inherited phenotypes to offspring and influence embryonic development.
Grace S. Lee, Natalie A. Trigg, Colin C. Conine   +2 more
doaj   +1 more source

Group data showing the effect of iGLUR blockade in the PVN on ARCN responses.

, 2013
A: In barodenervated rats, before iGLUR blockade in the PVN (open bar), microinjections of NMDA (10 mM, 20 nl) into the ARCN elicited increases in MAP (n = 6).
Tetsuya Kawabe (292023), Hreday N. Sapru (292027), Kazumi Kawabe (292025)   +2 more
core   +1 more source